DE69217623T2 - Nachweis von amplifizierten Nukleinsäuren unter Verwendung von sekundären wasserlöschlichen Helper-Oligonukleotiden und Testsatz dafür - Google Patents

Nachweis von amplifizierten Nukleinsäuren unter Verwendung von sekundären wasserlöschlichen Helper-Oligonukleotiden und Testsatz dafür

Info

Publication number
DE69217623T2
DE69217623T2 DE69217623T DE69217623T DE69217623T2 DE 69217623 T2 DE69217623 T2 DE 69217623T2 DE 69217623 T DE69217623 T DE 69217623T DE 69217623 T DE69217623 T DE 69217623T DE 69217623 T2 DE69217623 T2 DE 69217623T2
Authority
DE
Germany
Prior art keywords
detection
nucleic acids
test kit
secondary water
amplified nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69217623T
Other languages
English (en)
Other versions
DE69217623D1 (de
Inventor
Annie Liu Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Publication of DE69217623D1 publication Critical patent/DE69217623D1/de
Application granted granted Critical
Publication of DE69217623T2 publication Critical patent/DE69217623T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
DE69217623T 1991-10-02 1992-09-30 Nachweis von amplifizierten Nukleinsäuren unter Verwendung von sekundären wasserlöschlichen Helper-Oligonukleotiden und Testsatz dafür Expired - Lifetime DE69217623T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/769,624 US5387510A (en) 1991-10-02 1991-10-02 Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit

Publications (2)

Publication Number Publication Date
DE69217623D1 DE69217623D1 (de) 1997-04-03
DE69217623T2 true DE69217623T2 (de) 1998-01-02

Family

ID=25086024

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69217623T Expired - Lifetime DE69217623T2 (de) 1991-10-02 1992-09-30 Nachweis von amplifizierten Nukleinsäuren unter Verwendung von sekundären wasserlöschlichen Helper-Oligonukleotiden und Testsatz dafür

Country Status (7)

Country Link
US (1) US5387510A (de)
EP (1) EP0546590B1 (de)
JP (1) JP3497518B2 (de)
CA (1) CA2075683A1 (de)
DE (1) DE69217623T2 (de)
HK (1) HK1000253A1 (de)
SG (1) SG87728A1 (de)

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US5610274A (en) * 1991-11-20 1997-03-11 Cpg, Inc. Production and use of magnetic porous inorganic materials
US7524456B1 (en) 1992-05-21 2009-04-28 Biosite Incorporated Diagnostic devices for the controlled movement of reagents without membranes
US6905882B2 (en) * 1992-05-21 2005-06-14 Biosite, Inc. Diagnostic devices and apparatus for the controlled movement of reagents without membranes
US6767510B1 (en) * 1992-05-21 2004-07-27 Biosite, Inc. Diagnostic devices and apparatus for the controlled movement of reagents without membranes
FR2701961B1 (fr) * 1993-02-24 1995-04-21 Bio Merieux Procédé de destabilisation d'une structure secondaire intracaténaire d'un polynucléotide simple brin, et de capture dudit nucléotide.
FR2710075B1 (fr) * 1993-09-15 1995-10-27 Bio Merieux Réactif et procédé pour la détection d'une séquence nucléotidique avec amplification de signal.
US5591580A (en) * 1994-03-31 1997-01-07 Johnson & Johnson Clinical Diagnostics, Inc. Method, test element and test kit for semi-quantitative detection of target nucleic acid
US5834181A (en) * 1994-07-28 1998-11-10 Genzyme Corporation High throughput screening method for sequences or genetic alterations in nucleic acids
CA2163393C (en) * 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5683875A (en) * 1995-05-04 1997-11-04 Hewlett-Packard Company Method for detecting a target nucleic acid analyte in a sample
AU744032B2 (en) * 1995-06-07 2002-02-14 Bayer Corporation Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates and enzyme inhibition assays
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
EP0838025B1 (de) * 1995-07-13 2007-04-25 Applera Corporation Unabhängiges gerät zur extraktion, amplifikation und nachweis von nukleinsäuren
US5770365A (en) * 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
US5811253A (en) * 1995-09-01 1998-09-22 Johnson & Johnson Clinical Diagnostics, Inc. Use of vanadium bromoperoxidase as a signal-generating enzyme for chemiluminescent systems: test kits and analytical methods
GB9620075D0 (en) 1996-09-26 1996-11-13 Dynal As Method
US6110676A (en) * 1996-12-04 2000-08-29 Boston Probes, Inc. Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays
AU6435898A (en) * 1997-02-21 1998-09-09 Saigene Corporation Compositions and methods for determining predisposition to insulin dependent diabetes mellitus
US5994076A (en) * 1997-05-21 1999-11-30 Clontech Laboratories, Inc. Methods of assaying differential expression
US6489455B2 (en) 1997-05-21 2002-12-03 Clontech Laboratories, Inc. Methods of assaying differential expression
US6962778B1 (en) 1997-09-25 2005-11-08 Boston Probes, Inc. Methods, kits and compositions for suppressing the binding of detectable probes to non-target sequences in hybridization assays
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
CA2525413C (en) 2003-05-19 2010-08-17 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample
JP5249581B2 (ja) * 2004-08-09 2013-07-31 ジェネレイション バイオテック リミテッド ライアビリティ カンパニー 核酸の単離および増幅方法
CN101970111B (zh) 2007-06-21 2013-09-11 简·探针公司 用于执行处理的仪器和容器
CA2702276C (en) * 2007-10-09 2019-04-02 Hsueh-Chia Chang Microfluidic platforms for multi-target detection
US8790879B2 (en) 2010-01-22 2014-07-29 Gen-Probe Incorporated Probes for detecting the presence of Trichomonas vaginalis in a sample
WO2013119956A1 (en) * 2012-02-09 2013-08-15 Life Technologies Corporation Conjugated polymeric particle and method of making same
US10144968B2 (en) 2015-07-02 2018-12-04 Life Technologies Corporation Conjugation of carboxyl functional hydrophilic beads
US10150992B2 (en) 2015-07-06 2018-12-11 Life Technologies Corporation Substrates and methods useful in sequencing
CA3108105A1 (en) 2018-08-08 2020-02-13 Gen-Probe Incorporated Compositions, methods and kits for detecting mycoplasma genitalium
WO2021041056A1 (en) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4755458A (en) * 1984-08-30 1988-07-05 Enzo Biochem, Inc. Composition and method for the detection of the presence of a polynucleotide sequence of interest
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5079351A (en) * 1986-11-26 1992-01-07 Cetus Corporation Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
US5030557A (en) * 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization

Also Published As

Publication number Publication date
HK1000253A1 (en) 1998-02-13
JP3497518B2 (ja) 2004-02-16
EP0546590A1 (de) 1993-06-16
DE69217623D1 (de) 1997-04-03
SG87728A1 (en) 2002-04-16
JPH05211899A (ja) 1993-08-24
US5387510A (en) 1995-02-07
CA2075683A1 (en) 1993-04-03
EP0546590B1 (de) 1997-02-26

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Legal Events

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8332 No legal effect for de
8370 Indication of lapse of patent is to be deleted
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: BOEHMERT & BOEHMERT, 28209 BREMEN