DE69217197T2 - Vorrichtung und verfahren zur verbesserten probenkonzentrierung bei kapillarelektrophorese - Google Patents

Vorrichtung und verfahren zur verbesserten probenkonzentrierung bei kapillarelektrophorese

Info

Publication number
DE69217197T2
DE69217197T2 DE69217197T DE69217197T DE69217197T2 DE 69217197 T2 DE69217197 T2 DE 69217197T2 DE 69217197 T DE69217197 T DE 69217197T DE 69217197 T DE69217197 T DE 69217197T DE 69217197 T2 DE69217197 T2 DE 69217197T2
Authority
DE
Germany
Prior art keywords
capillary electrophoresis
sample concentration
improved sample
improved
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69217197T
Other languages
English (en)
Other versions
DE69217197D1 (de
Inventor
Ring-Ling Chien
Dean Burgi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Varian Inc
Original Assignee
Varian Associates Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Varian Associates Inc filed Critical Varian Associates Inc
Publication of DE69217197D1 publication Critical patent/DE69217197D1/de
Application granted granted Critical
Publication of DE69217197T2 publication Critical patent/DE69217197T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69217197T 1991-10-04 1992-09-30 Vorrichtung und verfahren zur verbesserten probenkonzentrierung bei kapillarelektrophorese Expired - Fee Related DE69217197T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/771,575 US5116471A (en) 1991-10-04 1991-10-04 System and method for improving sample concentration in capillary electrophoresis
PCT/US1992/008371 WO1993007478A1 (en) 1991-10-04 1992-09-30 System and method for improving sample concentration in capillary electrophoresis

Publications (2)

Publication Number Publication Date
DE69217197D1 DE69217197D1 (de) 1997-03-13
DE69217197T2 true DE69217197T2 (de) 1997-05-22

Family

ID=25092260

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69217197T Expired - Fee Related DE69217197T2 (de) 1991-10-04 1992-09-30 Vorrichtung und verfahren zur verbesserten probenkonzentrierung bei kapillarelektrophorese

Country Status (5)

Country Link
US (1) US5116471A (de)
EP (1) EP0560974B1 (de)
JP (1) JPH06505563A (de)
DE (1) DE69217197T2 (de)
WO (1) WO1993007478A1 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7273749B1 (en) * 1990-06-04 2007-09-25 University Of Utah Research Foundation Container for carrying out and monitoring biological processes
US6787338B2 (en) 1990-06-04 2004-09-07 The University Of Utah Method for rapid thermal cycling of biological samples
US5935522A (en) * 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US5340452A (en) * 1991-02-01 1994-08-23 Beckman Instruments, Inc. On-column preconcentration of samples in capillary electrophoresis
CA2144174A1 (en) * 1992-09-14 1994-03-31 Jianmin Bao Electrophoretically mediated chemical analysis
US5958202A (en) * 1992-09-14 1999-09-28 Perseptive Biosystems, Inc. Capillary electrophoresis enzyme immunoassay
US5376249A (en) * 1992-11-25 1994-12-27 Perseptive Biosystems, Inc. Analysis utilizing isoelectric focusing
US5505831A (en) * 1993-01-26 1996-04-09 Bio-Rad Laboratories, Inc. Concentration of biological samples on a microliter scale and analysis by capillary electrophoresis
AU663260B2 (en) * 1993-05-05 1995-09-28 Amrad Operations Pty. Limited Electrophoretic resolution of charged molecules
WO1994025144A1 (en) * 1993-05-05 1994-11-10 Amrad Corporation Limited Electrophoretic resolution of charged molecules
US5573651A (en) * 1995-04-17 1996-11-12 The Dow Chemical Company Apparatus and method for flow injection analysis
NZ502323A (en) * 1996-06-04 2001-09-28 Univ Utah Res Found Monitoring a fluorescence energy transfer pair during hybridization of first probe labelled with fluorescein to second probe labelled with Cy5 or Cy5.5
US5779868A (en) * 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
WO1998000705A1 (en) 1996-06-28 1998-01-08 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
US5869004A (en) 1997-06-09 1999-02-09 Caliper Technologies Corp. Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems
US6890411B1 (en) 1998-06-11 2005-05-10 Arizona Board Of Regents Control of flow and materials for micro devices
US6062261A (en) * 1998-12-16 2000-05-16 Lockheed Martin Energy Research Corporation MicrofluIdic circuit designs for performing electrokinetic manipulations that reduce the number of voltage sources and fluid reservoirs
US6428666B1 (en) 1999-02-22 2002-08-06 Sandia National Laboratories Electrokinetic concentration of charged molecules
US6322683B1 (en) 1999-04-14 2001-11-27 Caliper Technologies Corp. Alignment of multicomponent microfabricated structures
US6790328B2 (en) * 2000-01-12 2004-09-14 Ut-Battelle, Llc Microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
US6875348B2 (en) * 2000-02-18 2005-04-05 The Board Of Trustees Of The Leland Stanford Junior University Separation column having a photopolymerized sol-gel component and associated methods
US6866785B2 (en) * 2001-08-13 2005-03-15 The Board Of Trustees Of The Leland Stanford Junior University Photopolymerized sol-gel column and associated methods
EP1126275A3 (de) * 2000-02-18 2002-12-18 The Board Of Trustees Of The Leland Stanford Junior University Kapillarsäule aus geschmolzenem Siliziumdioxid mit Photopolymerbestandteilen
US20030057092A1 (en) * 2000-10-31 2003-03-27 Caliper Technologies Corp. Microfluidic methods, devices and systems for in situ material concentration
US20050011761A1 (en) * 2000-10-31 2005-01-20 Caliper Technologies Corp. Microfluidic methods, devices and systems for in situ material concentration
ATE432466T1 (de) * 2000-10-31 2009-06-15 Caliper Life Sciences Inc Mikrofluidisches verfahren für in-situ- materialkonzentration
US20020070166A1 (en) * 2000-12-07 2002-06-13 Board Of Governors Of The University Of Alberta Sample purification on a microfluidic device
US6881317B2 (en) * 2000-12-18 2005-04-19 The Trustees Of Princeton University Fractionation of macro-molecules using asymmetric pulsed field electrophoresis
US20030062833A1 (en) * 2001-10-03 2003-04-03 Wen-Yen Tai Mini-type decorative bulb capable of emitting light through entire circumferential face
US7597791B2 (en) * 2001-10-19 2009-10-06 The Trustees Of Princeton University Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules
US6719535B2 (en) * 2002-01-31 2004-04-13 Eksigent Technologies, Llc Variable potential electrokinetic device
JP3780226B2 (ja) * 2002-05-31 2006-05-31 株式会社日立ハイテクノロジーズ 電気泳動装置、及び電気泳動方法
US7235164B2 (en) 2002-10-18 2007-06-26 Eksigent Technologies, Llc Electrokinetic pump having capacitive electrodes
US20040259269A1 (en) * 2003-06-20 2004-12-23 Groton Biosystems Method for detection of molecular species in a crude sample using capillary electrophoresis
US20050127002A1 (en) * 2003-12-12 2005-06-16 Zare Richard N. Immobilized-enzyme microreactor devices for characterization of biomolecular analytes and associated methods
WO2007062182A2 (en) * 2005-11-23 2007-05-31 Eksigent Technologies, Llp Electrokinetic pump designs and drug delivery systems
US20070284308A1 (en) * 2006-06-12 2007-12-13 Zare Richard N Immobilized-enzyme microreactor devices for characterization of biomolecular analytes and associated methods
US7867592B2 (en) 2007-01-30 2011-01-11 Eksigent Technologies, Inc. Methods, compositions and devices, including electroosmotic pumps, comprising coated porous surfaces
WO2009076134A1 (en) * 2007-12-11 2009-06-18 Eksigent Technologies, Llc Electrokinetic pump with fixed stroke volume
WO2011150424A1 (en) * 2010-05-28 2011-12-01 Mehdi Moini Handheld/portable ultrafast capillary electrophoresis with adjustable porous tip in conjunction with mass spectrometry
EP2704759A4 (de) 2011-05-05 2015-06-03 Eksigent Technologies Llc Gelkupplung für elektrokinetische abgabesysteme
CN104049025A (zh) * 2014-01-23 2014-09-17 杭州师范大学 微量注射泵驱动液流的毛细管电泳分析系统
CN109444246B (zh) * 2018-11-01 2023-11-21 宁波大学 一种基于电压极性转换的瞬态毛细管等速电泳装置及方法

Also Published As

Publication number Publication date
EP0560974A4 (en) 1994-06-08
DE69217197D1 (de) 1997-03-13
EP0560974B1 (de) 1997-01-29
EP0560974A1 (de) 1993-09-22
US5116471A (en) 1992-05-26
WO1993007478A1 (en) 1993-04-15
JPH06505563A (ja) 1994-06-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee
8327 Change in the person/name/address of the patent owner

Owner name: VARIAN, INC., PALO ALTO, CALIF., US