DE69118946D1 - Thermostabile Mutanten von neutraler Protease und Vorkehrungen zu deren Herstellung - Google Patents

Thermostabile Mutanten von neutraler Protease und Vorkehrungen zu deren Herstellung

Info

Publication number
DE69118946D1
DE69118946D1 DE69118946T DE69118946T DE69118946D1 DE 69118946 D1 DE69118946 D1 DE 69118946D1 DE 69118946 T DE69118946 T DE 69118946T DE 69118946 T DE69118946 T DE 69118946T DE 69118946 D1 DE69118946 D1 DE 69118946D1
Authority
DE
Germany
Prior art keywords
neutral protease
mutants
precautions
production
gly
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69118946T
Other languages
English (en)
Other versions
DE69118946T2 (de
Inventor
Y Ros Immaculada Margarit
Susanna Campagnoli
Roberto Gianna
Salvatore Toma
Guido Grandi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eni Tecnologie SpA
Original Assignee
Eniricerche SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche SpA filed Critical Eniricerche SpA
Application granted granted Critical
Publication of DE69118946D1 publication Critical patent/DE69118946D1/de
Publication of DE69118946T2 publication Critical patent/DE69118946T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69118946T 1990-02-23 1991-02-18 Thermostabile Mutanten von neutraler Protease und Vorkehrungen zu deren Herstellung Expired - Fee Related DE69118946T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT19474A IT1239733B (it) 1990-02-23 1990-02-23 Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione

Publications (2)

Publication Number Publication Date
DE69118946D1 true DE69118946D1 (de) 1996-05-30
DE69118946T2 DE69118946T2 (de) 1996-10-31

Family

ID=11158327

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69118946T Expired - Fee Related DE69118946T2 (de) 1990-02-23 1991-02-18 Thermostabile Mutanten von neutraler Protease und Vorkehrungen zu deren Herstellung

Country Status (6)

Country Link
US (1) US5252478A (de)
EP (1) EP0443476B1 (de)
AT (1) ATE137262T1 (de)
DE (1) DE69118946T2 (de)
DK (1) DK0443476T3 (de)
IT (1) IT1239733B (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3012377B2 (ja) * 1991-10-11 2000-02-21 名糖産業株式会社 低耐熱性プロテアーゼとその関連物及びその生産法
GB9618960D0 (en) 1996-09-11 1996-10-23 Medical Science Sys Inc Proteases
US20030190644A1 (en) 1999-10-13 2003-10-09 Andreas Braun Methods for generating databases and databases for identifying polymorphic genetic markers
EP1613723B1 (de) 2002-11-27 2013-05-15 Sequenom, Inc. Auf fragmentierung beruhende verfahren zum nachweis und zur entdeckung von sequenzvariationen
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
CA2561381C (en) 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
CN101680872B (zh) * 2007-04-13 2015-05-13 塞昆纳姆股份有限公司 序列比较分析方法和系统
CN103865907B (zh) * 2013-12-13 2016-01-20 湖南鸿鹰生物科技有限公司 一种强热稳定性的中性蛋白酶
CN111944790B (zh) * 2020-07-01 2022-09-09 深圳润康生态环境股份有限公司 中性蛋白酶基因、中性蛋白酶及其制备方法和应用
CN113151329B (zh) * 2021-03-30 2023-09-08 云南师范大学 中性蛋白酶突变体及其构造方法和应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
IT1176997B (it) * 1984-10-17 1987-08-26 Anic Spa Procedimento per la produzione di proteasi neutra
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
US5041378A (en) * 1987-08-11 1991-08-20 Cetus Corporation Procaryotic xylose isomerase muteins
US4966846A (en) * 1987-10-01 1990-10-30 W. R. Grace & Co.-Conn. Molecular cloning and expression of a vibrio proteolyticus neutral protease gene

Also Published As

Publication number Publication date
EP0443476A3 (en) 1992-01-15
DE69118946T2 (de) 1996-10-31
EP0443476B1 (de) 1996-04-24
ATE137262T1 (de) 1996-05-15
IT9019474A0 (it) 1990-02-23
US5252478A (en) 1993-10-12
DK0443476T3 (da) 1996-05-13
IT9019474A1 (it) 1991-08-24
EP0443476A2 (de) 1991-08-28
IT1239733B (it) 1993-11-15

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee