DK0443476T3 - Termostabile mutanter af neutral protease og midler til fremstilling af disse - Google Patents

Termostabile mutanter af neutral protease og midler til fremstilling af disse

Info

Publication number
DK0443476T3
DK0443476T3 DK91102234.1T DK91102234T DK0443476T3 DK 0443476 T3 DK0443476 T3 DK 0443476T3 DK 91102234 T DK91102234 T DK 91102234T DK 0443476 T3 DK0443476 T3 DK 0443476T3
Authority
DK
Denmark
Prior art keywords
neutral protease
mutants
agents
production
gly
Prior art date
Application number
DK91102234.1T
Other languages
English (en)
Inventor
Guido Grandi
Y Ros Immaculada Margarit
Susanna Campagnoli
Roberto Gianna
Salvatore Toma
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Application granted granted Critical
Publication of DK0443476T3 publication Critical patent/DK0443476T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
DK91102234.1T 1990-02-23 1991-02-18 Termostabile mutanter af neutral protease og midler til fremstilling af disse DK0443476T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT19474A IT1239733B (it) 1990-02-23 1990-02-23 Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione

Publications (1)

Publication Number Publication Date
DK0443476T3 true DK0443476T3 (da) 1996-05-13

Family

ID=11158327

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91102234.1T DK0443476T3 (da) 1990-02-23 1991-02-18 Termostabile mutanter af neutral protease og midler til fremstilling af disse

Country Status (6)

Country Link
US (1) US5252478A (da)
EP (1) EP0443476B1 (da)
AT (1) ATE137262T1 (da)
DE (1) DE69118946T2 (da)
DK (1) DK0443476T3 (da)
IT (1) IT1239733B (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3012377B2 (ja) * 1991-10-11 2000-02-21 名糖産業株式会社 低耐熱性プロテアーゼとその関連物及びその生産法
GB9618960D0 (en) 1996-09-11 1996-10-23 Medical Science Sys Inc Proteases
US7332275B2 (en) 1999-10-13 2008-02-19 Sequenom, Inc. Methods for detecting methylated nucleotides
CA2507189C (en) 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
WO2005024068A2 (en) 2003-09-05 2005-03-17 Sequenom, Inc. Allele-specific sequence variation analysis
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
EP2395098B1 (en) 2004-03-26 2015-07-15 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
CA2684217C (en) * 2007-04-13 2016-12-13 Sequenom, Inc. Comparative sequence analysis processes and systems
CN103865907B (zh) * 2013-12-13 2016-01-20 湖南鸿鹰生物科技有限公司 一种强热稳定性的中性蛋白酶
CN111944790B (zh) * 2020-07-01 2022-09-09 深圳润康生态环境股份有限公司 中性蛋白酶基因、中性蛋白酶及其制备方法和应用
CN113151329B (zh) * 2021-03-30 2023-09-08 云南师范大学 中性蛋白酶突变体及其构造方法和应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
IT1176997B (it) * 1984-10-17 1987-08-26 Anic Spa Procedimento per la produzione di proteasi neutra
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
US5041378A (en) * 1987-08-11 1991-08-20 Cetus Corporation Procaryotic xylose isomerase muteins
US4966846A (en) * 1987-10-01 1990-10-30 W. R. Grace & Co.-Conn. Molecular cloning and expression of a vibrio proteolyticus neutral protease gene

Also Published As

Publication number Publication date
IT9019474A1 (it) 1991-08-24
EP0443476B1 (en) 1996-04-24
IT9019474A0 (it) 1990-02-23
DE69118946T2 (de) 1996-10-31
EP0443476A3 (en) 1992-01-15
DE69118946D1 (de) 1996-05-30
EP0443476A2 (en) 1991-08-28
IT1239733B (it) 1993-11-15
ATE137262T1 (de) 1996-05-15
US5252478A (en) 1993-10-12

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