DE69330340T2 - Mutanten von einer thermostabilen neutralen Protease aus Bacillus - Google Patents

Mutanten von einer thermostabilen neutralen Protease aus Bacillus

Info

Publication number
DE69330340T2
DE69330340T2 DE69330340T DE69330340T DE69330340T2 DE 69330340 T2 DE69330340 T2 DE 69330340T2 DE 69330340 T DE69330340 T DE 69330340T DE 69330340 T DE69330340 T DE 69330340T DE 69330340 T2 DE69330340 T2 DE 69330340T2
Authority
DE
Germany
Prior art keywords
mutants
bacillus
neutral protease
acid residue
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69330340T
Other languages
English (en)
Other versions
DE69330340D1 (de
Inventor
Hiromasa Nagao
Takashi Yoneya
Toshio Miyake
Atsuo Aoyama
Ken-Ichi Kai
Shun-Ichi Kidokoro
Yoichiro Miki
Kimiko Endo
Akiyoshi Wada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sagami Chemical Research Institute
Holland Sweetener Co VOF
Original Assignee
Sagami Chemical Research Institute
Holland Sweetener Co VOF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sagami Chemical Research Institute, Holland Sweetener Co VOF filed Critical Sagami Chemical Research Institute
Application granted granted Critical
Publication of DE69330340D1 publication Critical patent/DE69330340D1/de
Publication of DE69330340T2 publication Critical patent/DE69330340T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69330340T 1993-03-17 1993-03-17 Mutanten von einer thermostabilen neutralen Protease aus Bacillus Expired - Fee Related DE69330340T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP93200773A EP0616033B1 (de) 1993-03-17 1993-03-17 Mutanten von einer thermostabilen neutralen Protease aus Bacillus

Publications (2)

Publication Number Publication Date
DE69330340D1 DE69330340D1 (de) 2001-07-19
DE69330340T2 true DE69330340T2 (de) 2002-05-29

Family

ID=8213707

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69330340T Expired - Fee Related DE69330340T2 (de) 1993-03-17 1993-03-17 Mutanten von einer thermostabilen neutralen Protease aus Bacillus

Country Status (4)

Country Link
EP (1) EP0616033B1 (de)
AT (1) ATE202142T1 (de)
DE (1) DE69330340T2 (de)
DK (1) DK0616033T3 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100344204B1 (ko) * 1993-12-07 2002-11-30 자이단호오징 사가미 츄오 카가쿠겡큐쇼 신규의프로테아제ii
WO1997021804A1 (fr) * 1995-12-11 1997-06-19 Sagami Chemical Research Center Nouvelle protease analogue a la thermolysine et son utilisation
US9828597B2 (en) 2006-11-22 2017-11-28 Toyota Motor Engineering & Manufacturing North America, Inc. Biofunctional materials
US8569034B2 (en) * 2007-11-01 2013-10-29 Danisco Us Inc. Thermolysin variants and detergent compositions therewith
US11015149B2 (en) 2010-06-21 2021-05-25 Toyota Motor Corporation Methods of facilitating removal of a fingerprint
US9388370B2 (en) 2010-06-21 2016-07-12 Toyota Motor Engineering & Manufacturing North America, Inc. Thermolysin-like protease for cleaning insect body stains
US9121016B2 (en) 2011-09-09 2015-09-01 Toyota Motor Engineering & Manufacturing North America, Inc. Coatings containing polymer modified enzyme for stable self-cleaning of organic stains
US8796009B2 (en) * 2010-06-21 2014-08-05 Toyota Motor Engineering & Manufacturing North America, Inc. Clearcoat containing thermolysin-like protease from Bacillus stearothermophilus for cleaning of insect body stains
US10988714B2 (en) 2010-06-21 2021-04-27 Regents Of The University Of Minnesota Methods of facilitating removal of a fingerprint from a substrate or a coating

Also Published As

Publication number Publication date
EP0616033B1 (de) 2001-06-13
EP0616033A1 (de) 1994-09-21
DK0616033T3 (da) 2001-10-22
ATE202142T1 (de) 2001-06-15
DE69330340D1 (de) 2001-07-19

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee