ATE202142T1 - Mutanten von einer thermostabilen neutralen protease aus bacillus - Google Patents

Mutanten von einer thermostabilen neutralen protease aus bacillus

Info

Publication number
ATE202142T1
ATE202142T1 AT93200773T AT93200773T ATE202142T1 AT E202142 T1 ATE202142 T1 AT E202142T1 AT 93200773 T AT93200773 T AT 93200773T AT 93200773 T AT93200773 T AT 93200773T AT E202142 T1 ATE202142 T1 AT E202142T1
Authority
AT
Austria
Prior art keywords
mutants
bacillus
neutral protease
acid residue
amino acid
Prior art date
Application number
AT93200773T
Other languages
English (en)
Inventor
Hiromasa Nagao
Takashi Yoneya
Toshio Miyake
Atsuo Aoyama
Ken-Ichi Kai
Shun-Ichi Kidokoro
Yoichiro Miki
Kimiko Endo
Akiyoshi Wada
Original Assignee
Holland Sweetener Co
Sagami Chem Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Holland Sweetener Co, Sagami Chem Res filed Critical Holland Sweetener Co
Application granted granted Critical
Publication of ATE202142T1 publication Critical patent/ATE202142T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
AT93200773T 1993-03-17 1993-03-17 Mutanten von einer thermostabilen neutralen protease aus bacillus ATE202142T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP93200773A EP0616033B1 (de) 1993-03-17 1993-03-17 Mutanten von einer thermostabilen neutralen Protease aus Bacillus

Publications (1)

Publication Number Publication Date
ATE202142T1 true ATE202142T1 (de) 2001-06-15

Family

ID=8213707

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93200773T ATE202142T1 (de) 1993-03-17 1993-03-17 Mutanten von einer thermostabilen neutralen protease aus bacillus

Country Status (4)

Country Link
EP (1) EP0616033B1 (de)
AT (1) ATE202142T1 (de)
DE (1) DE69330340T2 (de)
DK (1) DK0616033T3 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1141649A (zh) * 1993-12-07 1997-01-29 财团法人相模中央化学研究所 源于芽孢杆菌的热稳定性天然蛋白酶变体
EP0896056A4 (de) * 1995-12-11 2001-08-29 Sagami Chem Res Neue thermolysin-ähnliche protease und ihre verwendung.
US9828597B2 (en) 2006-11-22 2017-11-28 Toyota Motor Engineering & Manufacturing North America, Inc. Biofunctional materials
CA2704311C (en) * 2007-11-01 2018-02-13 Danisco Us Inc. Production of thermolysin and variants thereof, and use in liquid detergents
US8796009B2 (en) * 2010-06-21 2014-08-05 Toyota Motor Engineering & Manufacturing North America, Inc. Clearcoat containing thermolysin-like protease from Bacillus stearothermophilus for cleaning of insect body stains
US11015149B2 (en) 2010-06-21 2021-05-25 Toyota Motor Corporation Methods of facilitating removal of a fingerprint
US10988714B2 (en) 2010-06-21 2021-04-27 Regents Of The University Of Minnesota Methods of facilitating removal of a fingerprint from a substrate or a coating
US9388370B2 (en) 2010-06-21 2016-07-12 Toyota Motor Engineering & Manufacturing North America, Inc. Thermolysin-like protease for cleaning insect body stains
US9121016B2 (en) 2011-09-09 2015-09-01 Toyota Motor Engineering & Manufacturing North America, Inc. Coatings containing polymer modified enzyme for stable self-cleaning of organic stains

Also Published As

Publication number Publication date
DE69330340T2 (de) 2002-05-29
DK0616033T3 (da) 2001-10-22
EP0616033A1 (de) 1994-09-21
DE69330340D1 (de) 2001-07-19
EP0616033B1 (de) 2001-06-13

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