DE69011101D1 - Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung. - Google Patents

Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.

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Publication number
DE69011101D1
DE69011101D1 DE69011101T DE69011101T DE69011101D1 DE 69011101 D1 DE69011101 D1 DE 69011101D1 DE 69011101 T DE69011101 T DE 69011101T DE 69011101 T DE69011101 T DE 69011101T DE 69011101 D1 DE69011101 D1 DE 69011101D1
Authority
DE
Germany
Prior art keywords
genomic dna
cohesive
restriction
mapping
linker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69011101T
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English (en)
Other versions
DE69011101T2 (de
Inventor
Harvey Miller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DE69011101D1 publication Critical patent/DE69011101D1/de
Application granted granted Critical
Publication of DE69011101T2 publication Critical patent/DE69011101T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Control And Other Processes For Unpacking Of Materials (AREA)
DE69011101T 1989-03-03 1990-02-15 Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung. Expired - Lifetime DE69011101T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31838589A 1989-03-03 1989-03-03
PCT/US1990/000942 WO1990010064A1 (en) 1989-03-03 1990-02-15 Improved methods for in vitro dna amplification and genomic cloning and mapping

Publications (2)

Publication Number Publication Date
DE69011101D1 true DE69011101D1 (de) 1994-09-01
DE69011101T2 DE69011101T2 (de) 1995-01-26

Family

ID=23237960

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69011101T Expired - Lifetime DE69011101T2 (de) 1989-03-03 1990-02-15 Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.
DE69034197T Expired - Lifetime DE69034197T2 (de) 1989-03-03 1990-02-15 Genomische Klonierung und Kartographie

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69034197T Expired - Lifetime DE69034197T2 (de) 1989-03-03 1990-02-15 Genomische Klonierung und Kartographie

Country Status (7)

Country Link
EP (2) EP0593095B1 (de)
JP (1) JP3030417B2 (de)
AT (2) ATE109204T1 (de)
DE (2) DE69011101T2 (de)
DK (2) DK0593095T3 (de)
ES (1) ES2058893T3 (de)
WO (1) WO1990010064A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
DE3929030A1 (de) * 1989-09-01 1991-03-07 Boehringer Mannheim Gmbh Verfahren zur vermehrung von nukleinsaeuren
DE4106473C2 (de) * 1991-03-01 1994-03-10 Boehringer Mannheim Gmbh Verfahren zur Herstellung in vitro replizierbarer Nukleinsäuren
US20100267023A1 (en) 1992-09-24 2010-10-21 Keygene N.V. Selective restriction fragment amplification: fingerprinting
IT1262937B (it) * 1992-04-13 1996-07-22 Enichem Spa Selezione genica in vitro mediante vettori ad rna
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
AT402203B (de) * 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
US5550025A (en) * 1995-07-19 1996-08-27 Becton, Dickinson And Company Detection of hydrophobic amplification products by extraction into an organic phase
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6136537A (en) * 1998-02-23 2000-10-24 Macevicz; Stephen C. Gene expression analysis
US6054276A (en) 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US8367322B2 (en) 1999-01-06 2013-02-05 Cornell Research Foundation, Inc. Accelerating identification of single nucleotide polymorphisms and alignment of clones in genomic sequencing
EP1141384A2 (de) 1999-01-06 2001-10-10 Cornell Research Foundation, Inc. Verfahren zur beschleunigung der identifizierung von mononukleotid-polymorphismen und der ausrichtung von klonen bei genomischer sequenzierung
GB0114852D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Happiar mapping
US7229800B2 (en) 2004-04-16 2007-06-12 Becton, Dickinson And Company Neisseria gonorrhoeae assay
WO2011020075A2 (en) 2009-08-13 2011-02-17 Life Technologies Corporation Amelogenin snp on chromosome x
US9029088B2 (en) 2009-10-15 2015-05-12 Life Technologies Corporation Human single nucleotide polymorphisms within CODIS loci D13S317, TH01, vWA, D12S391, and D6S1043
US20110165568A1 (en) 2009-12-31 2011-07-07 Life Technologies Corporation Sequences of e.coli 055:h7 genome
US9090946B2 (en) 2010-09-21 2015-07-28 Life Technologies Corporation SE33 mutations impacting genotype concordance
CN103282511A (zh) 2010-11-01 2013-09-04 贝克顿·迪金森公司 阴道加特纳菌测定
ES2387295B1 (es) * 2011-03-03 2013-07-31 Consejo Superior De Investigaciones Científicas (Csic) MÉTODO DE AMPLIFICACIÓN DE ADN BASADO EN LOS ORÍGENES DE REPLICACIÓN DEL BACTERIÓFAGO Phi29 Y SECUENCIAS NUCLEOTÍDICAS ASOCIADAS.
EP2721170A2 (de) 2011-06-17 2014-04-23 Life Technologies Corporation Zusammensetzungen und verfahren für den nachweis von cronobacter spp. und cronobacter-spezies und stämmen
US20130122505A1 (en) 2011-08-24 2013-05-16 Life Technologies Corporation Compositions and methods for detection of multiple microorganisms
WO2013151647A1 (en) 2012-04-02 2013-10-10 Life Technologies Corporation Compositions and methods for detection of mycobacterium avium paratuberculosis
US20140005061A1 (en) 2012-06-29 2014-01-02 Life Technologies Corporation Compositions and methods for detection of multiple microorganisms
EP2867377B1 (de) 2012-07-02 2019-08-21 The Translational Genomics Research Institute Primer, assays und verfahren zum nachweis eines e.-coli-subtyps
US10844441B2 (en) 2016-05-16 2020-11-24 Life Technologies Corporation Penta e polymorphisms for human identification

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve

Also Published As

Publication number Publication date
DK0593095T3 (da) 2005-10-17
ES2058893T3 (es) 1994-11-01
EP0593095B1 (de) 2005-06-29
ATE109204T1 (de) 1994-08-15
ATE298795T1 (de) 2005-07-15
WO1990010064A1 (en) 1990-09-07
EP0461155B1 (de) 1994-07-27
JP3030417B2 (ja) 2000-04-10
EP0593095A1 (de) 1994-04-20
DK0461155T3 (da) 1994-11-21
JPH04503752A (ja) 1992-07-09
DE69011101T2 (de) 1995-01-26
DE69034197D1 (de) 2005-08-04
DE69034197T2 (de) 2006-04-27
EP0461155A1 (de) 1991-12-18

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