DE68924186T2 - Füllstoff für die Messung von Enzym-Aktivität, mit dem Füllstoff verpackte Säule und Verfahren zur Messung von Enzym-Aktivität durch Gebrauch der Säule. - Google Patents

Füllstoff für die Messung von Enzym-Aktivität, mit dem Füllstoff verpackte Säule und Verfahren zur Messung von Enzym-Aktivität durch Gebrauch der Säule.

Info

Publication number
DE68924186T2
DE68924186T2 DE68924186T DE68924186T DE68924186T2 DE 68924186 T2 DE68924186 T2 DE 68924186T2 DE 68924186 T DE68924186 T DE 68924186T DE 68924186 T DE68924186 T DE 68924186T DE 68924186 T2 DE68924186 T2 DE 68924186T2
Authority
DE
Germany
Prior art keywords
filler
enzyme activity
measuring enzyme
column
column packed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE68924186T
Other languages
English (en)
Other versions
DE68924186D1 (de
Inventor
Tamami Koyama
Soyao Moriguchi
Hiroshi Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Resonac Holdings Corp
Original Assignee
Showa Denko KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP63250340A external-priority patent/JPH0728756B2/ja
Priority claimed from JP31615688A external-priority patent/JPH02163097A/ja
Priority claimed from JP706589A external-priority patent/JPH02186997A/ja
Application filed by Showa Denko KK filed Critical Showa Denko KK
Application granted granted Critical
Publication of DE68924186D1 publication Critical patent/DE68924186D1/de
Publication of DE68924186T2 publication Critical patent/DE68924186T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
    • C12Q2337/10—Anilides
    • C12Q2337/12—Para-Nitroanilides p-NA
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81—Protease inhibitors
    • G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
    • G01N2333/8121—Serpins
    • G01N2333/8128—Antithrombin III
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/976—Trypsin; Chymotrypsin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE68924186T 1988-02-19 1989-02-20 Füllstoff für die Messung von Enzym-Aktivität, mit dem Füllstoff verpackte Säule und Verfahren zur Messung von Enzym-Aktivität durch Gebrauch der Säule. Expired - Fee Related DE68924186T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP3698788 1988-02-19
JP63250340A JPH0728756B2 (ja) 1988-02-19 1988-10-04 酵素活性測定用充填剤、それを充填したカラムおよびそれを用いる酵素活性測定方法
JP31615688A JPH02163097A (ja) 1988-12-16 1988-12-16 阻害剤の共存下における酵素活性測定方法
JP706589A JPH02186997A (ja) 1989-01-13 1989-01-13 酵素選別方法

Publications (2)

Publication Number Publication Date
DE68924186D1 DE68924186D1 (de) 1995-10-19
DE68924186T2 true DE68924186T2 (de) 1996-05-02

Family

ID=27454626

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68924186T Expired - Fee Related DE68924186T2 (de) 1988-02-19 1989-02-20 Füllstoff für die Messung von Enzym-Aktivität, mit dem Füllstoff verpackte Säule und Verfahren zur Messung von Enzym-Aktivität durch Gebrauch der Säule.

Country Status (4)

Country Link
US (1) US5654152A (de)
EP (1) EP0329190B1 (de)
CA (1) CA1339812C (de)
DE (1) DE68924186T2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04229168A (ja) * 1990-12-26 1992-08-18 Hoechst Japan Ltd 酵素の活性と酵素反応阻害物質の活性を測定するための器具及び方法
AU708196B2 (en) * 1995-06-20 1999-07-29 Johannes Schumacher Process and device for determining the activity of enzymes in liquids, or the concentration and/or activity of inhibitors in liquids
CA2248558A1 (en) * 1996-03-15 1997-09-18 Yale University Methods and means of detecting nitric oxide synthase
CA2189486A1 (en) * 1996-11-04 1998-05-04 Yves St-Pierre Analysis of enzyme activity using immobilized fluorescence-labeled substrates
US5976178A (en) * 1996-11-07 1999-11-02 Vascular Science Inc. Medical grafting methods
CA2279378C (en) * 1997-02-04 2005-10-11 Morten Miller Method of selectively determining a fungal biomass
US20030068655A1 (en) * 2001-09-12 2003-04-10 Protiveris, Inc. Microcantilever apparatus and methods for detection of enzymes
JPWO2005118842A1 (ja) * 2004-06-01 2008-04-03 シスメックス株式会社 酵素活性測定方法および酵素活性測定用カラム
US20090075298A1 (en) * 2005-05-20 2009-03-19 Mitsubishi Kagaku Iatron, Inc. Method of analyzing enzyme
WO2007073597A1 (en) 2005-12-27 2007-07-05 Mcmaster University Enzyme measurement assay using a modified substrate comprising a substrate attached to a macromolecule via a spacer
US9091700B2 (en) 2005-12-27 2015-07-28 Mcmaster University Modified peptide substrate
WO2007106762A2 (en) * 2006-03-14 2007-09-20 Washington University In St. Louis Alzheimer's disease biomarkers and methods of use
US20110143380A1 (en) * 2006-03-14 2011-06-16 The Washington University Alzheimer's disease biomarkers and methods of use
DE112008002473A5 (de) 2007-07-06 2010-06-17 Papst Licensing Gmbh & Co. Kg Bestimmung der Aktivität von Proteasen
DE102009004371A1 (de) 2009-01-08 2010-07-15 Papst Licensing Gmbh & Co. Kg Vorrichtung und Verfahren zum Messen der Aktivität von Enzymen nach Inhibitorentzug
GB201002824D0 (en) * 2010-02-19 2010-04-07 Temasek Polytechnic A method of preparing a substrate for immobilization of functional substances thereon and the substrate obtained therefrom
WO2011143574A2 (en) 2010-05-14 2011-11-17 The Trustees Of The University Of Pennsylvania Plasma biomarkers for diagnosis of alzheimer's disease
CN110354817A (zh) * 2019-07-01 2019-10-22 西安交通大学 一种基于固定化凝血酶的亲和色谱柱及其制备方法和应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4046633A (en) * 1974-07-01 1977-09-06 University Of Utah Direct renin assay system and method
US3933589A (en) * 1975-03-20 1976-01-20 Owens-Illinois, Inc. Chemical immobilization of enzymes
US4169765A (en) * 1977-09-14 1979-10-02 Technicon Instruments Corp. Apparatus for the quantitative determination of α-amylase
US4172765A (en) * 1977-09-14 1979-10-30 Technicon Instruments Corporation Method for the quantitative determination of alpha-amylase
US4337309A (en) * 1978-02-28 1982-06-29 Pharmacia Diagnostics Ab Method of deterining the concentration of pancreatic and salivary α-amylase in body fluids
US4217415A (en) * 1978-03-14 1980-08-12 Technicon Instruments Corporation Immobilization of an enzyme substrate
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
DE3172131D1 (en) * 1980-06-27 1985-10-10 Akzo Nv Porous inorganic support material coated with an organic stationary phase, for use in chromatography, and process for its preparation
US4517241A (en) * 1982-12-02 1985-05-14 Alpert Andrew J Chromatographic support material
US4859581A (en) * 1986-03-10 1989-08-22 Board Of Regents, The University Of Texas System Endoglycosidase assay
US4973577A (en) * 1986-04-04 1990-11-27 The Salk Institute For Biological Studies FSH-releasing peptides
IL78678A0 (en) * 1986-05-04 1986-08-31 Yeda Res & Dev Substituted silica

Also Published As

Publication number Publication date
EP0329190A3 (de) 1991-03-27
EP0329190B1 (de) 1995-09-13
US5654152A (en) 1997-08-05
DE68924186D1 (de) 1995-10-19
CA1339812C (en) 1998-04-14
EP0329190A2 (de) 1989-08-23

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee