DE68920987T2 - Rekombinante DNA enthaltende Expressionsvektoren und für Isopenicillin-N-Epimerase-(Racemase)-Aktivität kodierende DNS-Verbindungen. - Google Patents

Rekombinante DNA enthaltende Expressionsvektoren und für Isopenicillin-N-Epimerase-(Racemase)-Aktivität kodierende DNS-Verbindungen.

Info

Publication number
DE68920987T2
DE68920987T2 DE68920987T DE68920987T DE68920987T2 DE 68920987 T2 DE68920987 T2 DE 68920987T2 DE 68920987 T DE68920987 T DE 68920987T DE 68920987 T DE68920987 T DE 68920987T DE 68920987 T2 DE68920987 T2 DE 68920987T2
Authority
DE
Germany
Prior art keywords
racemase
epimerase
activity
dna
expression vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE68920987T
Other languages
English (en)
Other versions
DE68920987D1 (de
Inventor
Steven Kovacevic
James Robert Miller
Paul Luther Skatrud
Matthew Barry Tobin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of DE68920987D1 publication Critical patent/DE68920987D1/de
Publication of DE68920987T2 publication Critical patent/DE68920987T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE68920987T 1988-12-22 1989-12-15 Rekombinante DNA enthaltende Expressionsvektoren und für Isopenicillin-N-Epimerase-(Racemase)-Aktivität kodierende DNS-Verbindungen. Expired - Fee Related DE68920987T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US28876088A 1988-12-22 1988-12-22

Publications (2)

Publication Number Publication Date
DE68920987D1 DE68920987D1 (de) 1995-03-16
DE68920987T2 true DE68920987T2 (de) 1995-06-22

Family

ID=23108528

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68920987T Expired - Fee Related DE68920987T2 (de) 1988-12-22 1989-12-15 Rekombinante DNA enthaltende Expressionsvektoren und für Isopenicillin-N-Epimerase-(Racemase)-Aktivität kodierende DNS-Verbindungen.

Country Status (12)

Country Link
EP (1) EP0377295B1 (de)
JP (1) JPH02227082A (de)
AT (1) ATE118042T1 (de)
AU (1) AU622253B2 (de)
CA (1) CA2005649A1 (de)
DE (1) DE68920987T2 (de)
DK (1) DK641489A (de)
ES (1) ES2067556T3 (de)
GR (1) GR3015473T3 (de)
HU (1) HU208713B (de)
IE (1) IE894168L (de)
IL (1) IL92740A0 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL94651A0 (en) * 1989-06-12 1991-04-15 Univ Oklahoma State Purified enzyme and process therefor
CA2034735A1 (en) * 1990-01-26 1991-07-27 Barbara J. Weigel Recombinant dna expression and dna compounds that encode isopenicillin n epimerase activity
ATE334203T1 (de) * 1994-09-28 2006-08-15 Novozymes As Verfahren zur herstellung von sekundären metaboliten
FR2744455B1 (fr) 1996-02-02 1998-04-24 Sanofi Sa Nouvelles sequences d'acides nucleiques de la famille des facteurs suppresseurs de tumeurs, produits d'expression correspondants et leurs applications
US7030227B1 (en) 1997-10-15 2006-04-18 President & Fellows Of Harvard College Cell regulatory genes, encoded products, and uses related thereto
US7294459B1 (en) 1997-10-15 2007-11-13 President And Fellows Of Harvard College Cell regulatory genes, encoded products, and uses related thereto
WO2010077806A1 (en) 2008-12-15 2010-07-08 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
KR20130071433A (ko) 2010-05-07 2013-06-28 그린라이트 바이오사이언시스, 아이엔씨. 효소의 재배치를 통한 대사 경로에서의 흐름의 제어 방법
JP6280367B2 (ja) 2010-08-31 2018-02-14 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． プロテアーゼ操作を介した代謝経路におけるフラックスの制御のための方法
CA2846893A1 (en) 2011-09-09 2013-03-14 Greenlight Biosciences, Inc. Cell-free preparation of carbapenems
BR112016002494A2 (pt) 2013-08-05 2017-09-05 Greenlight Biosciences Inc Proteí-nas construídas com um sítio de clivagem de protease, ácido nucléico, vetor, célula e processo de engenharia de uma proteína recombinante e de um grande número de variantes de ácidos nucleicos que codificam proteínas recombinantes
MX2017012665A (es) 2015-03-30 2018-04-24 Greenlight Biosciences Inc Produccion de acido ribonucleico libre de celulas.
EP4293104A3 (de) 2016-04-06 2024-04-24 Greenlight Biosciences, Inc. Zellfreie herstellung von ribonukleinsäure
KR20230130144A (ko) 2017-10-11 2023-09-11 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0233175A1 (de) * 1984-11-21 1987-08-26 Medicor Vorrichtung zur regelung der öffnungszeiten eines x-strahlengerätes

Also Published As

Publication number Publication date
CA2005649A1 (en) 1990-06-22
IE894168L (en) 1990-06-22
AU4709889A (en) 1990-06-28
EP0377295B1 (de) 1995-02-01
IL92740A0 (en) 1990-09-17
HU208713B (en) 1993-12-28
HU896734D0 (en) 1990-02-28
GR3015473T3 (en) 1995-06-30
HUT53149A (en) 1990-09-28
DK641489D0 (da) 1989-12-18
ATE118042T1 (de) 1995-02-15
AU622253B2 (en) 1992-04-02
JPH02227082A (ja) 1990-09-10
DK641489A (da) 1990-06-23
ES2067556T3 (es) 1995-04-01
EP0377295A1 (de) 1990-07-11
DE68920987D1 (de) 1995-03-16

Similar Documents

Publication Publication Date Title
ATE118042T1 (de) Rekombinante dna enthaltende expressionsvektoren und für isopenicillin-n-epimerase-(racemase)- aktivität kodierende dns-verbindungen.
AU5233686A (en) Dna sequence encoding a hirudin-like protein and process for preparing such protein
EP0246307A4 (de) Durch cys-kodon modifizierte dns.
FI905660A0 (fi) Nya polypeptider, dna-sekvenser som moejliggoer deras expression, foerfarande foer framstaellning och anvaendning.
AU592791B2 (en) Recombinant DNA expression vectors and DNA compounds which encode isopenicillin N synthetase
DK0444775T3 (da) Rekombinant-DNA-ekspressionsvektorer og DNA-forbindelser, som koder for isopenicillin-N-epimerase-ak tivitet
DK606088A (da) Rekombinant-dna-udtrykkelsesvektorer og dna-forbindelser, der koder for isopenicillin n synthetase
FR2681076B1 (fr) Adn recombinant codant pour une proteine a activite endochitinase.
DK260188D0 (da) Enzymproduktion
DK0422790T3 (da) Rekombinant DNA-ekspressionsvektorer og DNA-forbindelser der koder for acyltransferaseaktivitet fra Aspergillus
NO178704C (no) DNA-sekvens som koder for protein med IRF-1-aktivitet, ekspresjonsvektor inneholdende denne, anvendelse av IRF-1 for å öke ekspresjon, vertscelle transformert med ekspresjonsvektoren, og protein med IRF-1-aktivitet

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee