DE68915841D1 - Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation. - Google Patents

Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation.

Info

Publication number
DE68915841D1
DE68915841D1 DE68915841T DE68915841T DE68915841D1 DE 68915841 D1 DE68915841 D1 DE 68915841D1 DE 68915841 T DE68915841 T DE 68915841T DE 68915841 T DE68915841 T DE 68915841T DE 68915841 D1 DE68915841 D1 DE 68915841D1
Authority
DE
Germany
Prior art keywords
preparation
growth factor
epidermal growth
genetic transformation
genetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE68915841T
Other languages
English (en)
Other versions
DE68915841T2 (de
Inventor
Norio Shimizu
Yoshinori Harada
Shinichi Fukuzono
Kiyoshi Fujimori
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Showa Denko Materials Co ltd
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP63074382A external-priority patent/JPH01247098A/ja
Priority claimed from JP63074383A external-priority patent/JPH01247099A/ja
Application filed by Hitachi Chemical Co Ltd, Hitachi Ltd filed Critical Hitachi Chemical Co Ltd
Publication of DE68915841D1 publication Critical patent/DE68915841D1/de
Application granted granted Critical
Publication of DE68915841T2 publication Critical patent/DE68915841T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF] (urogastrone)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE1989615841 1988-03-30 1989-03-30 Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation. Expired - Fee Related DE68915841T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP63074382A JPH01247098A (ja) 1988-03-30 1988-03-30 ヒトの上皮細胞増殖因子の遺伝子工学的生産方法
JP63074383A JPH01247099A (ja) 1988-03-30 1988-03-30 ヒトの上皮細胞増殖因子の遺伝子工学的生産方法

Publications (2)

Publication Number Publication Date
DE68915841D1 true DE68915841D1 (de) 1994-07-14
DE68915841T2 DE68915841T2 (de) 1995-02-02

Family

ID=26415525

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1989615841 Expired - Fee Related DE68915841T2 (de) 1988-03-30 1989-03-30 Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation.

Country Status (2)

Country Link
EP (1) EP0335400B1 (de)
DE (1) DE68915841T2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03244385A (ja) * 1990-02-21 1991-10-31 Hitachi Ltd ヒト神経成長因子の遺伝子工学的生産方法
WO1994025592A1 (en) * 1993-04-26 1994-11-10 Daewoong Pharmaceutical Co., Ltd. A novel gene coding human epidermal growth factor and process for preparing the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
JPS59154989A (ja) * 1983-02-23 1984-09-04 Mitsubishi Chem Ind Ltd プラスミド
DE3484926D1 (de) * 1983-12-23 1991-09-19 Pfizer Expressionsplasmide fuer die herstellung eines heterologen proteins in bakterien.
CA1263619A (en) * 1984-10-09 1989-12-05 Ryuji Marumoto Dna, production and use thereof
GB8507666D0 (en) * 1985-03-25 1985-05-01 Wellcome Found Epidermal growth factor production
DE3852074D1 (de) * 1987-03-20 1994-12-15 Creative Biomolecules Inc Verfahren zur reinigung von rekombinanten polypeptiden.

Also Published As

Publication number Publication date
EP0335400B1 (de) 1994-06-08
EP0335400A3 (en) 1990-06-20
EP0335400A2 (de) 1989-10-04
DE68915841T2 (de) 1995-02-02

Similar Documents

Publication Publication Date Title
DE68913922T2 (de) Verfahren zur Herstellung von niedrigen aliphatischen Kohlenwasserstoffen.
DE69005542D1 (de) Verfahren zur Herstellung von 3-Pentensäure.
DE69021239T2 (de) Verfahren zur Herstellung von ellagischer Säure.
DE68913447T2 (de) Verfahren zur Herstellung von Halofluorbenzolen.
DE59003697D1 (de) Verfahren zur Herstellung von Chromsäure.
DE69020119T2 (de) Verfahren zur Herstellung von hochreiner Naphthalin-2,6-Dicarbonsäure.
DE68922275T2 (de) Verfahren zur Herstellung von Monochloroacetaldehyde-Trimer.
DE3885262D1 (de) Verfahren zur Herstellung von 2-Chlor-5-methyl-pyridin.
DE68911603D1 (de) Verfahren zur Herstellung von 2-Pyrrolidon.
DE59204060D1 (de) Verfahren zur Herstellung von 1-Fluor-cyclopropan-1-carbonsäure.
DE68920298D1 (de) Verfahren zur Herstellung von 4-Acetoxyazetidinonen.
DE68915841T2 (de) Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation.
DE58905664D1 (de) Verfahren zur Herstellung von N-Hydroxypyrazolen.
DE58905592D1 (de) Verfahren zur Herstellung von N-Hydroxypyrazolen.
ATE73765T1 (de) Verfahren zur herstellung von 2-nitro-5-phenoxy-n-alkylsulfonylbenzamiden durch nitrierung.
DE68919584D1 (de) Verfahren zur Herstellung von Chlorfluorbenzolen.
DE58907097D1 (de) Verfahren zur Herstellung von 4-Chlorpyrazolen.
DE68916768D1 (de) Verfahren zur Herstellung von para-Hydroxybenzoesäure.
DE68916375T2 (de) Verfahren zur Herstellung von 3-Iminonitrilen.
DE59007620D1 (de) Verfahren zur Herstellung von 3-Methylchinolin-8-carbonsäure.
DE59003699D1 (de) Verfahren zur Herstellung von Chromsäure.
DE68922361D1 (de) Verfahren zur Herstellung von 2,6-Naphthalendicarbonsäure.
DE69116198D1 (de) Verfahren zur Herstellung von D-Isocitronensäure durch Fermentation
DE69020091D1 (de) Verfahren zur Herstellung von 2-Trifluormethyl-4-hydroxybenzoesäure.
DE58907865D1 (de) Verfahren zur Herstellung von alpha-Ketocarbonsäureestern.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee