DE60333673D1 - Überwachungsprotein zur messung der proteinverarbeitung - Google Patents

Überwachungsprotein zur messung der proteinverarbeitung

Info

Publication number
DE60333673D1
DE60333673D1 DE60333673T DE60333673T DE60333673D1 DE 60333673 D1 DE60333673 D1 DE 60333673D1 DE 60333673 T DE60333673 T DE 60333673T DE 60333673 T DE60333673 T DE 60333673T DE 60333673 D1 DE60333673 D1 DE 60333673D1
Authority
DE
Germany
Prior art keywords
protein
monitor
measuring
processing
protein processing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60333673T
Other languages
English (en)
Inventor
Yoshihiro Ohmiya
Emiko Ashitaka
Seiji Ito
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Original Assignee
Japan Science and Technology Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science and Technology Agency filed Critical Japan Science and Technology Agency
Application granted granted Critical
Publication of DE60333673D1 publication Critical patent/DE60333673D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
DE60333673T 2002-12-12 2003-12-11 Überwachungsprotein zur messung der proteinverarbeitung Expired - Lifetime DE60333673D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002360744 2002-12-12
PCT/JP2003/015828 WO2004052934A1 (ja) 2002-12-12 2003-12-11 蛋白質のプロセッシングを測定するためのモニター蛋白質

Publications (1)

Publication Number Publication Date
DE60333673D1 true DE60333673D1 (de) 2010-09-16

Family

ID=32501001

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60333673T Expired - Lifetime DE60333673D1 (de) 2002-12-12 2003-12-11 Überwachungsprotein zur messung der proteinverarbeitung

Country Status (7)

Country Link
US (1) US20070224647A2 (de)
EP (1) EP1580196B1 (de)
JP (1) JP4427671B2 (de)
CN (1) CN1726228B (de)
AU (1) AU2003289316A1 (de)
DE (1) DE60333673D1 (de)
WO (1) WO2004052934A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7989621B2 (en) 2005-09-26 2011-08-02 National Institute Of Advanced Industrial Science And Technology Method for producing substituted imidazo[1,2-A]pyrazines of (s)-1-(3-(2-sec-butyl-6-(1h-indol-3-yl)-3-oxo-3,7-dihydroimadazo[1,2-a]pyrazin-8-yl)propyl)guanidine
US8487035B2 (en) 2006-03-30 2013-07-16 Asahi Kasei Chemicals Corporation Resin composition and molded product thereof
US8147842B2 (en) 2006-06-12 2012-04-03 National Institute Of Advanced Industrial Science And Technology Mutant luciferase
JP5995372B2 (ja) 2011-03-15 2016-09-21 オリンパス株式会社 星虫由来ルシフェラーゼ
CN103562719B (zh) 2011-03-16 2016-01-13 国立大学法人东北大学 生物体组织分析用探针及其使用方法
WO2016152882A1 (ja) * 2015-03-20 2016-09-29 国立大学法人東京工業大学 コラーゲン融合タンパク質、及びそれを用いた薬剤のスクリーニング方法
CN115011621A (zh) * 2022-06-27 2022-09-06 齐鲁工业大学 一种重组蛋白及荧光检测底物的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6228604B1 (en) * 1996-04-10 2001-05-08 Loma Linda University Secreted renilla luciferase
WO1999066324A2 (en) * 1998-06-16 1999-12-23 Biosignal Packard Inc. A bioluminescence resonance energy transfer (bret) system and its use
JP4830063B2 (ja) * 2000-03-15 2011-12-07 プロルーム・リミテッド Ｒｅｎｉｌｌａｒｅｎｉｆｏｒｍｉｓ蛍光タンパク質、その蛍光タンパク質をコードする核酸、および診断、ハイスループットスクリーニングおよび新規アイテムにおけるその使用
EP1419390B1 (de) * 2000-09-25 2007-11-14 U.S. Medical Research Institute of Infectious Diseases Hoch-durchsatz assays für proteolytische aktivitäten von clostridium neurotoxinen
JPWO2004022600A1 (ja) * 2002-09-06 2005-12-22 独立行政法人産業技術総合研究所 分泌型又は膜結合型キメラ蛋白質

Also Published As

Publication number Publication date
EP1580196B1 (de) 2010-08-04
US20060035286A1 (en) 2006-02-16
JP4427671B2 (ja) 2010-03-10
EP1580196A4 (de) 2006-06-21
CN1726228A (zh) 2006-01-25
JPWO2004052934A1 (ja) 2006-04-13
AU2003289316A8 (en) 2004-06-30
US20070224647A2 (en) 2007-09-27
WO2004052934A1 (ja) 2004-06-24
EP1580196A1 (de) 2005-09-28
CN1726228B (zh) 2010-05-26
AU2003289316A1 (en) 2004-06-30

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