DE60237850D1 - KONSTRUKTION NEUER STÄMME MIT MINIMALISIERUNGSGENOM UNTER VERWENDUNG DES Tn5-GEKOPPTELTEN Cre/loxP-EXZISIONSSYSTEMS - Google Patents

KONSTRUKTION NEUER STÄMME MIT MINIMALISIERUNGSGENOM UNTER VERWENDUNG DES Tn5-GEKOPPTELTEN Cre/loxP-EXZISIONSSYSTEMS

Info

Publication number
DE60237850D1
DE60237850D1 DE60237850T DE60237850T DE60237850D1 DE 60237850 D1 DE60237850 D1 DE 60237850D1 DE 60237850 T DE60237850 T DE 60237850T DE 60237850 T DE60237850 T DE 60237850T DE 60237850 D1 DE60237850 D1 DE 60237850D1
Authority
DE
Germany
Prior art keywords
chromosome
cre
loxp
transposon
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60237850T
Other languages
English (en)
Inventor
Sun-Chang Kim
Byung-Jo Yu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Advanced Institute of Science and Technology KAIST
Original Assignee
Korea Advanced Institute of Science and Technology KAIST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Advanced Institute of Science and Technology KAIST filed Critical Korea Advanced Institute of Science and Technology KAIST
Application granted granted Critical
Publication of DE60237850D1 publication Critical patent/DE60237850D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE60237850T 2002-02-22 2002-10-31 KONSTRUKTION NEUER STÄMME MIT MINIMALISIERUNGSGENOM UNTER VERWENDUNG DES Tn5-GEKOPPTELTEN Cre/loxP-EXZISIONSSYSTEMS Expired - Lifetime DE60237850D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2002-0009647A KR100459870B1 (ko) 2002-02-22 2002-02-22 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법
PCT/KR2002/002033 WO2003070955A1 (en) 2002-02-22 2002-10-31 CONSTRUCTION OF NOVEL STRAINS CONTAINING MINIMIZING GENOME BY Tn5-COUPLED Cre/loxp EXCISION SYSTEM

Publications (1)

Publication Number Publication Date
DE60237850D1 true DE60237850D1 (de) 2010-11-11

Family

ID=27751920

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60237850T Expired - Lifetime DE60237850D1 (de) 2002-02-22 2002-10-31 KONSTRUKTION NEUER STÄMME MIT MINIMALISIERUNGSGENOM UNTER VERWENDUNG DES Tn5-GEKOPPTELTEN Cre/loxP-EXZISIONSSYSTEMS

Country Status (8)

Country Link
US (1) US7745218B2 (de)
EP (1) EP1487982B1 (de)
JP (1) JP4303597B2 (de)
KR (1) KR100459870B1 (de)
AT (1) ATE483025T1 (de)
AU (1) AU2002367694A1 (de)
DE (1) DE60237850D1 (de)
WO (1) WO2003070955A1 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1921140B1 (de) * 2005-07-05 2011-12-14 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Mutanter transposonvektor und verwendung davon
GB201107515D0 (en) 2011-05-05 2011-06-22 Discuva Ltd Method for identifying antibiotic targets
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
GB201219989D0 (en) * 2012-11-06 2012-12-19 Discuva Ltd Bacterial engineering
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124336A2 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Partitioning and processing of analytes and other species
EP3470530B1 (de) 2013-05-23 2020-11-25 The Board of Trustees of the Leland Stanford Junior University Transposition in natives chromatin zur analyse des chromatins
CN110548550B (zh) 2014-04-10 2022-03-08 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
CA2953374A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US10961298B2 (en) 2014-08-21 2021-03-30 The Government Of The United States, Represented By The Secretary Of The Army Monoclonal antibodies for treatment of microbial infections
AU2015339148B2 (en) 2014-10-29 2022-03-10 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
MX367432B (es) 2015-01-12 2019-08-08 10X Genomics Inc Procesos y sistemas para la preparación de bibliotecas de secuenciación de ácido nucleico y bibliotecas preparadas con estos.
CA2975958A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP4286516A3 (de) 2015-02-24 2024-03-06 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183A3 (de) 2017-01-30 2024-02-21 10X Genomics, Inc. Verfahren und systeme zur tröpfchenbasierten einzelzellenbarcodierung
EP4230746A3 (de) 2017-05-26 2023-11-01 10X Genomics, Inc. Einzelzellanalyse von transposase-zugänglichem chromatin
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3625361A1 (de) 2017-11-15 2020-03-25 10X Genomics, Inc. Funktionalisierte gelperlen
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CN109161559A (zh) * 2018-09-04 2019-01-08 浙江大学 一种高效的链霉菌基因组精简系统的构建及应用
CN114934059B (zh) * 2022-03-04 2023-02-21 深圳先进技术研究院 高通量简化噬菌体基因组骨架的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5925545A (en) * 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
WO2001018222A1 (en) * 1999-09-07 2001-03-15 University Of Florida Methods and compositions for the chromosomal integration of heterologous sequences

Also Published As

Publication number Publication date
KR20030069708A (ko) 2003-08-27
JP4303597B2 (ja) 2009-07-29
EP1487982B1 (de) 2010-09-29
KR100459870B1 (ko) 2004-12-04
US20090104682A1 (en) 2009-04-23
JP2005517447A (ja) 2005-06-16
AU2002367694A1 (en) 2003-09-09
EP1487982A1 (de) 2004-12-22
US7745218B2 (en) 2010-06-29
ATE483025T1 (de) 2010-10-15
EP1487982A4 (de) 2005-06-29
WO2003070955A1 (en) 2003-08-28

Similar Documents

Publication Publication Date Title
DE60237850D1 (de) KONSTRUKTION NEUER STÄMME MIT MINIMALISIERUNGSGENOM UNTER VERWENDUNG DES Tn5-GEKOPPTELTEN Cre/loxP-EXZISIONSSYSTEMS
EA200000883A1 (ru) Способ сооружения, ремонта и/или ликвидации скважин
DK1141371T3 (da) Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller
EP1001338A3 (de) Verfahren und Gerät zur Software-Entwicklung
ATE553210T1 (de) Verfahren zur herstellung von l-threonin unter verwendung eines mikroorganismus mit inaktivierten galr gen.
CN108051281A (zh) 一种制作加预应力锚杆的含裂隙岩样的模具
DE60005984D1 (de) Verfahren und arbeitsmaschine zum verlegen von kabeln, rohren und dgl
DE60333777D1 (de) Verfahren zur herstellung eines betonbogens
GB2382367A (en) Liner hanger with standoffs
AU9357998A (en) Arched support structure
DE60129354D1 (de) Zusammensetzung zur herstellung eines fluorelastomers
WO2004056973A3 (en) Method for chromosomal engineering
NO20054936L (no) Biodegraderbar stivneretardator for sement og fremgangsmate ved sementering i undejordiske formasjoner
CN211501866U (zh) 一种人工顶管自纠偏装置
TWI263727B (en) A stiffening earth structure
ATE299932T1 (de) Verfahren der zellkultivierung
CN111460668A (zh) 一种基于bim技术的高速公路数字仿真建模方法
DE69607901D1 (de) Verfahren und Grabvorrichtung zur Herstellung von Tunnelsohlen
CN219939157U (zh) 一种市政工程用树木支撑装置
CN219343171U (zh) 一种基坑支护土钉墙结构
NO20010246L (no) Fremgangsmåte for fremstilling av proteiner i transformerte gjærceller
EP1079027A3 (de) Pfahlsystem
MXPA05013508A (es) Metodo para producir un polipeptido.
Park et al. A Study on the Influence of Ground Subsidence and Stability of Buildings by Tunnel Excavation in Urban Area using Numerical Analysis and Neural Network Method
DK1015577T3 (da) Kromosomal mutagenese i Pichia methanolica