ATE483025T1 - Konstruktion neuer stämme mit minimalisierungsgenom unter verwendung des tn5- gekopptelten cre/loxp-exzisionssystems - Google Patents

Konstruktion neuer stämme mit minimalisierungsgenom unter verwendung des tn5- gekopptelten cre/loxp-exzisionssystems

Info

Publication number
ATE483025T1
ATE483025T1 AT02790956T AT02790956T ATE483025T1 AT E483025 T1 ATE483025 T1 AT E483025T1 AT 02790956 T AT02790956 T AT 02790956T AT 02790956 T AT02790956 T AT 02790956T AT E483025 T1 ATE483025 T1 AT E483025T1
Authority
AT
Austria
Prior art keywords
chromosome
cre
transposon
minimalization
genome
Prior art date
Application number
AT02790956T
Other languages
English (en)
Inventor
Sun-Chang Kim
Byung-Jo Yu
Original Assignee
Korea Advanced Inst Sci & Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Advanced Inst Sci & Tech filed Critical Korea Advanced Inst Sci & Tech
Application granted granted Critical
Publication of ATE483025T1 publication Critical patent/ATE483025T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT02790956T 2002-02-22 2002-10-31 Konstruktion neuer stämme mit minimalisierungsgenom unter verwendung des tn5- gekopptelten cre/loxp-exzisionssystems ATE483025T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2002-0009647A KR100459870B1 (ko) 2002-02-22 2002-02-22 트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법
PCT/KR2002/002033 WO2003070955A1 (en) 2002-02-22 2002-10-31 CONSTRUCTION OF NOVEL STRAINS CONTAINING MINIMIZING GENOME BY Tn5-COUPLED Cre/loxp EXCISION SYSTEM

Publications (1)

Publication Number Publication Date
ATE483025T1 true ATE483025T1 (de) 2010-10-15

Family

ID=27751920

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02790956T ATE483025T1 (de) 2002-02-22 2002-10-31 Konstruktion neuer stämme mit minimalisierungsgenom unter verwendung des tn5- gekopptelten cre/loxp-exzisionssystems

Country Status (8)

Country Link
US (1) US7745218B2 (de)
EP (1) EP1487982B1 (de)
JP (1) JP4303597B2 (de)
KR (1) KR100459870B1 (de)
AT (1) ATE483025T1 (de)
AU (1) AU2002367694A1 (de)
DE (1) DE60237850D1 (de)
WO (1) WO2003070955A1 (de)

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EP1921140B1 (de) * 2005-07-05 2011-12-14 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Mutanter transposonvektor und verwendung davon
GB201107515D0 (en) 2011-05-05 2011-06-22 Discuva Ltd Method for identifying antibiotic targets
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
BR112015003354A8 (pt) 2012-08-14 2018-01-16 10X Genomics Inc métodos e composições de microcápsula
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
GB201219989D0 (en) * 2012-11-06 2012-12-19 Discuva Ltd Bacterial engineering
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124336A2 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Partitioning and processing of analytes and other species
EP3470530B1 (de) 2013-05-23 2020-11-25 The Board of Trustees of the Leland Stanford Junior University Transposition in natives chromatin zur analyse des chromatins
CN110548550B (zh) 2014-04-10 2022-03-08 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
CA2953374A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
US10961298B2 (en) 2014-08-21 2021-03-30 The Government Of The United States, Represented By The Secretary Of The Army Monoclonal antibodies for treatment of microbial infections
AU2015339148B2 (en) 2014-10-29 2022-03-10 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
MX367432B (es) 2015-01-12 2019-08-08 10X Genomics Inc Procesos y sistemas para la preparación de bibliotecas de secuenciación de ácido nucleico y bibliotecas preparadas con estos.
CA2975958A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP4286516A3 (de) 2015-02-24 2024-03-06 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183A3 (de) 2017-01-30 2024-02-21 10X Genomics, Inc. Verfahren und systeme zur tröpfchenbasierten einzelzellenbarcodierung
EP4230746A3 (de) 2017-05-26 2023-11-01 10X Genomics, Inc. Einzelzellanalyse von transposase-zugänglichem chromatin
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3625361A1 (de) 2017-11-15 2020-03-25 10X Genomics, Inc. Funktionalisierte gelperlen
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CN109161559A (zh) * 2018-09-04 2019-01-08 浙江大学 一种高效的链霉菌基因组精简系统的构建及应用
CN114934059B (zh) * 2022-03-04 2023-02-21 深圳先进技术研究院 高通量简化噬菌体基因组骨架的方法

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US5925545A (en) * 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
WO2001018222A1 (en) * 1999-09-07 2001-03-15 University Of Florida Methods and compositions for the chromosomal integration of heterologous sequences

Also Published As

Publication number Publication date
KR20030069708A (ko) 2003-08-27
JP4303597B2 (ja) 2009-07-29
EP1487982B1 (de) 2010-09-29
KR100459870B1 (ko) 2004-12-04
US20090104682A1 (en) 2009-04-23
JP2005517447A (ja) 2005-06-16
DE60237850D1 (de) 2010-11-11
AU2002367694A1 (en) 2003-09-09
EP1487982A1 (de) 2004-12-22
US7745218B2 (en) 2010-06-29
EP1487982A4 (de) 2005-06-29
WO2003070955A1 (en) 2003-08-28

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