DE60216245D1 - Funktionelles oberflächendisplay von polypeptiden - Google Patents

Funktionelles oberflächendisplay von polypeptiden

Info

Publication number
DE60216245D1
DE60216245D1 DE60216245T DE60216245T DE60216245D1 DE 60216245 D1 DE60216245 D1 DE 60216245D1 DE 60216245 T DE60216245 T DE 60216245T DE 60216245 T DE60216245 T DE 60216245T DE 60216245 D1 DE60216245 D1 DE 60216245D1
Authority
DE
Germany
Prior art keywords
polypeptides
surface display
functional surface
display
autotransporter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60216245T
Other languages
English (en)
Other versions
DE60216245T2 (de
Inventor
Joachim Jose
Franck Hannemann
Rita Bernhardt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zyrus Beteiligungs GmbH and Co Patente I KG
Original Assignee
UNIVERSITAET DES SAARLANDES WISSENS- und TECHNOLOGIETRANSFER GmbH
Universitaet des Saarlandes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UNIVERSITAET DES SAARLANDES WISSENS- und TECHNOLOGIETRANSFER GmbH, Universitaet des Saarlandes filed Critical UNIVERSITAET DES SAARLANDES WISSENS- und TECHNOLOGIETRANSFER GmbH
Publication of DE60216245D1 publication Critical patent/DE60216245D1/de
Application granted granted Critical
Publication of DE60216245T2 publication Critical patent/DE60216245T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
DE60216245T 2001-03-02 2002-03-01 Funktionelles oberflächendisplay von polypeptiden Expired - Lifetime DE60216245T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01105129 2001-03-02
EP01105129 2001-03-02
PCT/EP2002/002246 WO2002070645A2 (en) 2001-03-02 2002-03-01 Functional surface display of polypeptides

Publications (2)

Publication Number Publication Date
DE60216245D1 true DE60216245D1 (de) 2007-01-04
DE60216245T2 DE60216245T2 (de) 2007-06-21

Family

ID=8176655

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60216245T Expired - Lifetime DE60216245T2 (de) 2001-03-02 2002-03-01 Funktionelles oberflächendisplay von polypeptiden

Country Status (7)

Country Link
US (1) US20040259151A1 (de)
EP (1) EP1373571B1 (de)
AT (1) ATE346168T1 (de)
AU (1) AU2002249249A1 (de)
DE (1) DE60216245T2 (de)
DK (1) DK1373571T3 (de)
WO (1) WO2002070645A2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004065415A2 (en) 2003-01-20 2004-08-05 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. L-amino acid oxidase with cytotoxic activity from aplysia punctata
US7253293B2 (en) * 2004-01-06 2007-08-07 Andrzej Slominski Enzymatic method to produce 7-dehydropregnenolone, vitamin D3-like compounds and derivatives thereof
US8029777B2 (en) 2004-08-13 2011-10-04 Marshall Barry J Helicobacter system and uses thereof
JP2008509168A (ja) 2004-08-13 2008-03-27 マーシャル，バリー，ジェー． 細菌デリバリシステム
US20080124355A1 (en) 2006-09-22 2008-05-29 David Gordon Bermudes Live bacterial vaccines for viral infection prophylaxis or treatment
CN101889092A (zh) 2007-10-04 2010-11-17 生物结构实验室公司 生物燃料生产
US8241623B1 (en) 2009-02-09 2012-08-14 David Bermudes Protease sensitivity expression system
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
US8524220B1 (en) 2010-02-09 2013-09-03 David Gordon Bermudes Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
DE102010035702A1 (de) * 2010-08-27 2012-03-01 Zyrus Beteiligungsgesellschaft Mbh & Co. Patente I Kg Ganzzell-Biokatalysator
WO2012038508A1 (en) * 2010-09-23 2012-03-29 Autodisplay Biotech Gmbh Surface display of polypeptides containing a metal porphyrin or a flavin
PL2622078T3 (pl) * 2010-09-28 2015-05-29 Abera Bioscience Ab Białko fuzyjne dla ekspresji białka wydzielniczego
EP2675474B1 (de) 2011-02-15 2019-01-16 Vaxiion Therapeutics, LLC Therapeutische zusammensetzungen und verfahren für auf antikörper- und fc-haltigen molekülen basierende gezielte verabreichung bioaktiver moleküle durch bakterienminizellen
EP2707482B1 (de) * 2011-05-12 2015-09-09 Autodisplay Biotech GmbH Ganzzellen-biokatalysator mit einer prenyltransferase
ES2604335T3 (es) 2011-07-20 2017-03-06 Evonik Degussa Gmbh Oxidación y aminación de alcoholes primarios
US20140308717A1 (en) 2011-08-05 2014-10-16 Evonik Degussa Gmbh Oxidation and amination of secondary alcohols
EP2602328A1 (de) 2011-12-05 2013-06-12 Evonik Industries AG Verfahren zur Oxidation von Alkanen unter Verwendung einer AlkB Alkan 1-Monooxygenase
EP2602329A1 (de) 2011-12-05 2013-06-12 Evonik Degussa GmbH Biotechnologische Herstellung von 3-Hydroxyisobuttersäure
EP2639308A1 (de) 2012-03-12 2013-09-18 Evonik Industries AG Enzymatische omega-Oxidation und -Aminierung von Fettsäuren
EP2653538A1 (de) 2012-04-20 2013-10-23 Evonik Industries AG NADP-abhängige Alanindehydrogenase
US9593339B1 (en) 2013-02-14 2017-03-14 David Gordon Bermudes Bacteria carrying bacteriophage and protease inhibitors for the treatment of disorders and methods of treatment
WO2014139862A1 (en) * 2013-03-13 2014-09-18 Autodisplay Biotech Gmbh Improved surface display of functional proteins in a broad range of gram negative bacteria
US9737592B1 (en) 2014-02-14 2017-08-22 David Gordon Bermudes Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4344350C2 (de) * 1993-12-23 1995-09-21 Max Planck Gesellschaft Bakterien zur Herstellung stabiler Fusionsproteine und Verfahren zu deren Nachweis
US6686168B1 (en) * 1999-11-04 2004-02-03 Zymogenetics, Inc. Cell surface display of proteins by recombinant host cells

Also Published As

Publication number Publication date
WO2002070645A2 (en) 2002-09-12
ATE346168T1 (de) 2006-12-15
AU2002249249A1 (en) 2002-09-19
DK1373571T3 (da) 2007-04-16
WO2002070645A3 (en) 2003-07-24
EP1373571A2 (de) 2004-01-02
US20040259151A1 (en) 2004-12-23
EP1373571B1 (de) 2006-11-22
DE60216245T2 (de) 2007-06-21

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: ZYRUS BETEILIGUNGSGESELLSCHAFT MBH & CO. PATEN, DE