DE60202196D1 - Orientationsgerichtete konstruktion von plasmiden - Google Patents

Orientationsgerichtete konstruktion von plasmiden

Info

Publication number
DE60202196D1
DE60202196D1 DE2002602196 DE60202196T DE60202196D1 DE 60202196 D1 DE60202196 D1 DE 60202196D1 DE 2002602196 DE2002602196 DE 2002602196 DE 60202196 T DE60202196 T DE 60202196T DE 60202196 D1 DE60202196 D1 DE 60202196D1
Authority
DE
Germany
Prior art keywords
combined
orientation
segments
reaction
ligated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE2002602196
Other languages
English (en)
Other versions
DE60202196T2 (de
Inventor
Yitzhak Ben-Asouli
Farhat Osman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Bio Application Ltd
Original Assignee
Gene Bio Application Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Bio Application Ltd filed Critical Gene Bio Application Ltd
Application granted granted Critical
Publication of DE60202196D1 publication Critical patent/DE60202196D1/de
Publication of DE60202196T2 publication Critical patent/DE60202196T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60202196T 2001-02-12 2002-02-11 Orientationsgerichtete konstruktion von plasmiden Expired - Fee Related DE60202196T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IL14139201 2001-02-12
IL14139201A IL141392A0 (en) 2001-02-12 2001-02-12 Orientation-directed construction of plasmids
PCT/IL2002/000104 WO2002064774A2 (en) 2001-02-12 2002-02-11 Orientation-directed construction of plasmids

Publications (2)

Publication Number Publication Date
DE60202196D1 true DE60202196D1 (de) 2005-01-13
DE60202196T2 DE60202196T2 (de) 2005-12-29

Family

ID=11075124

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60202196T Expired - Fee Related DE60202196T2 (de) 2001-02-12 2002-02-11 Orientationsgerichtete konstruktion von plasmiden

Country Status (7)

Country Link
US (1) US7279278B2 (de)
EP (1) EP1362101B1 (de)
AT (1) ATE284441T1 (de)
DE (1) DE60202196T2 (de)
ES (1) ES2235013T3 (de)
IL (1) IL141392A0 (de)
WO (1) WO2002064774A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006047669A2 (en) * 2004-10-27 2006-05-04 Monsanto Technology Llc Non-random method of gene shuffling
CN106715710B (zh) 2013-03-15 2022-10-25 赛拉诺斯知识产权有限责任公司 核酸扩增
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
US9416387B2 (en) 2013-03-15 2016-08-16 Theranos, Inc. Nucleic acid amplification
US20140302504A1 (en) 2013-03-15 2014-10-09 Theranos, Inc. Nucleic Acid Amplification
US9529976B2 (en) 2013-09-06 2016-12-27 Theranos, Inc. Systems and methods for detecting infectious diseases

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4459359A (en) * 1981-11-19 1984-07-10 New York Blood Center, Inc. Sensitive immunoassays of antigens or antibodies sequestered within immune complexes
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5830713A (en) * 1986-11-04 1998-11-03 Protein Polymer Technologies, Inc. Methods for preparing synthetic repetitive DNA
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5523221A (en) * 1993-06-16 1996-06-04 Stratagene Method for the directional cloning of DNA
CA2188713A1 (en) * 1995-10-27 1997-04-28 Robert Collu Internal standards for quantitative competitive pcr
JP3415995B2 (ja) * 1996-06-10 2003-06-09 科学技術振興事業団 高分子マイクロ遺伝子重合体の作成方法
US6641998B2 (en) * 1997-10-10 2003-11-04 Stratagene Methods and kits to enrich for desired nucleic acid sequences
WO2000061741A1 (en) * 1999-04-12 2000-10-19 Genentech, Inc. Pcr-based cloning method

Also Published As

Publication number Publication date
WO2002064774A3 (en) 2002-12-19
DE60202196T2 (de) 2005-12-29
EP1362101A2 (de) 2003-11-19
EP1362101B1 (de) 2004-12-08
WO2002064774A2 (en) 2002-08-22
ATE284441T1 (de) 2004-12-15
US20040209272A1 (en) 2004-10-21
US7279278B2 (en) 2007-10-09
ES2235013T3 (es) 2005-07-01
IL141392A0 (en) 2002-03-10

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee