WO2020258084A9 - 巢式多重pcr高通量测序文库制备方法及试剂盒 - Google Patents
巢式多重pcr高通量测序文库制备方法及试剂盒 Download PDFInfo
- Publication number
- WO2020258084A9 WO2020258084A9 PCT/CN2019/093066 CN2019093066W WO2020258084A9 WO 2020258084 A9 WO2020258084 A9 WO 2020258084A9 CN 2019093066 W CN2019093066 W CN 2019093066W WO 2020258084 A9 WO2020258084 A9 WO 2020258084A9
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- primer
- sequence
- nested
- multiplex pcr
- throughput sequencing
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA3140403A CA3140403A1 (en) | 2019-06-26 | 2019-06-26 | Method for preparing nested multiplex pcr high-throughput sequencing library and kit |
KR1020217042321A KR20220011725A (ko) | 2019-06-26 | 2019-06-26 | 네스티드 다중 pcr 고처리량 시퀀싱 라이브러리의 제조 방법 및 키트 |
JP2021574288A JP7281565B2 (ja) | 2019-06-26 | 2019-06-26 | ネストされたマルチプレックスpcrハイスループットシーケンシングライブラリー調製方法及びキット |
AU2019453690A AU2019453690B2 (en) | 2019-06-26 | 2019-06-26 | Method for preparing nested multiplex PCR high-throughput sequencing library and kit |
PCT/CN2019/093066 WO2020258084A1 (zh) | 2019-06-26 | 2019-06-26 | 巢式多重pcr高通量测序文库制备方法及试剂盒 |
US17/617,854 US20220235414A1 (en) | 2019-06-26 | 2019-06-26 | Method for preparing high-throughput sequencing library based on nested mutiplex pcr and kit for the same |
EP19935242.8A EP3992303A4 (en) | 2019-06-26 | 2019-06-26 | METHOD FOR BUILDING A HIGH-THROUGH-THROUGH NESTING MULTIPLEX PCR SEQUENCING LIBRARY AND KIT |
CN201980095420.3A CN113811620B (zh) | 2019-06-26 | 2019-06-26 | 巢式多重pcr高通量测序文库制备方法及试剂盒 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2019/093066 WO2020258084A1 (zh) | 2019-06-26 | 2019-06-26 | 巢式多重pcr高通量测序文库制备方法及试剂盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2020258084A1 WO2020258084A1 (zh) | 2020-12-30 |
WO2020258084A9 true WO2020258084A9 (zh) | 2021-02-04 |
Family
ID=74059862
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/CN2019/093066 WO2020258084A1 (zh) | 2019-06-26 | 2019-06-26 | 巢式多重pcr高通量测序文库制备方法及试剂盒 |
Country Status (8)
Country | Link |
---|---|
US (1) | US20220235414A1 (zh) |
EP (1) | EP3992303A4 (zh) |
JP (1) | JP7281565B2 (zh) |
KR (1) | KR20220011725A (zh) |
CN (1) | CN113811620B (zh) |
AU (1) | AU2019453690B2 (zh) |
CA (1) | CA3140403A1 (zh) |
WO (1) | WO2020258084A1 (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114317696A (zh) * | 2021-12-24 | 2022-04-12 | 深圳裕康医学检验实验室 | 一种试剂盒及其文库构建方法与污染检测方法 |
WO2024049276A1 (ko) * | 2022-09-01 | 2024-03-07 | 주식회사 키오믹스 | 다중 표적 dna의 선택적 증폭용 조성물 및 이를 이용한 증폭 방법 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1990879A (zh) * | 2005-12-27 | 2007-07-04 | 广西大学 | 利用巢式pcr进行水牛胚胎性别鉴定的方法 |
CN101988124B (zh) * | 2010-09-03 | 2012-10-24 | 华东师范大学 | 巢式pcr法扩增egfr基因外显子19片段的方法 |
EP2559774A1 (en) * | 2011-08-17 | 2013-02-20 | Roche Diagniostics GmbH | Improved method for amplification of target nucleic acids using a multi-primer approach |
WO2013138510A1 (en) * | 2012-03-13 | 2013-09-19 | Patel Abhijit Ajit | Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing |
CN106192018B (zh) * | 2015-05-07 | 2020-03-24 | 深圳华大智造科技有限公司 | 一种锚定巢式多重pcr富集dna目标区域的方法和试剂盒 |
CN106282353B (zh) * | 2016-08-26 | 2019-12-10 | 上海翼和应用生物技术有限公司 | 一种利用发夹引物进行多重pcr的方法 |
WO2018044831A1 (en) * | 2016-08-30 | 2018-03-08 | Integrated Dna Technologies, Inc. | Cleavable hairpin primers |
CN117512078A (zh) | 2016-09-22 | 2024-02-06 | 西格马-奥尔德里奇有限责任公司 | 单引物至双引物扩增子转换 |
CN110114472A (zh) * | 2016-12-21 | 2019-08-09 | 深圳华大智造科技有限公司 | 将线性测序文库转换为环状测序文库的方法 |
CN108517354A (zh) * | 2017-02-28 | 2018-09-11 | 上海茂槿生物技术有限公司 | 用于多重pcr的引物组合物 |
WO2018170659A1 (en) * | 2017-03-20 | 2018-09-27 | Celula China Med-Technology Co., Ltd. | Methods and compositions for preparing sequencing libraries |
US10941445B2 (en) * | 2017-03-24 | 2021-03-09 | Bio-Rad Laboratories, Inc. | Universal hairpin primers |
US11305282B2 (en) | 2017-03-29 | 2022-04-19 | Cornell University | Devices, processes, and systems for determination of nucleic acid sequence, expression, copy number, or methylation changes using combined nuclease, ligase, polymerase, and sequencing reactions |
WO2018232597A1 (zh) * | 2017-06-20 | 2018-12-27 | 深圳华大智造科技有限公司 | 用于定量pcr扩增的组合物及其应用 |
CN107312847A (zh) * | 2017-07-13 | 2017-11-03 | 上海美吉生物医药科技有限公司 | 植物内生细菌多样性16SrDNA扩增子制备方法 |
CN109321642A (zh) | 2017-07-31 | 2019-02-12 | 广州康昕瑞基因健康科技有限公司 | 单管巢式pcr反应体系和扩增方法 |
WO2019023924A1 (en) * | 2017-08-01 | 2019-02-07 | Helitec Limited | METHODS FOR ENRICHMENT AND DETERMINATION OF TARGET NUCLEOTIDE SEQUENCES |
CN110669823B (zh) | 2018-07-03 | 2022-05-24 | 中国医学科学院肿瘤医院 | 一种同时检测多种肝癌常见突变的ctDNA文库构建和测序数据分析方法 |
CN109486921A (zh) * | 2018-12-26 | 2019-03-19 | 山东艾克韦生物技术有限公司 | 一种增加多重pcr引物兼容性的方法 |
-
2019
- 2019-06-26 KR KR1020217042321A patent/KR20220011725A/ko unknown
- 2019-06-26 CA CA3140403A patent/CA3140403A1/en active Pending
- 2019-06-26 JP JP2021574288A patent/JP7281565B2/ja active Active
- 2019-06-26 CN CN201980095420.3A patent/CN113811620B/zh active Active
- 2019-06-26 WO PCT/CN2019/093066 patent/WO2020258084A1/zh unknown
- 2019-06-26 US US17/617,854 patent/US20220235414A1/en active Pending
- 2019-06-26 AU AU2019453690A patent/AU2019453690B2/en active Active
- 2019-06-26 EP EP19935242.8A patent/EP3992303A4/en active Pending
Also Published As
Publication number | Publication date |
---|---|
EP3992303A4 (en) | 2023-03-15 |
AU2019453690B2 (en) | 2024-04-04 |
JP2022537284A (ja) | 2022-08-25 |
JP7281565B2 (ja) | 2023-05-25 |
AU2019453690A1 (en) | 2022-01-06 |
CN113811620A (zh) | 2021-12-17 |
EP3992303A1 (en) | 2022-05-04 |
CN113811620B (zh) | 2024-04-09 |
KR20220011725A (ko) | 2022-01-28 |
WO2020258084A1 (zh) | 2020-12-30 |
US20220235414A1 (en) | 2022-07-28 |
CA3140403A1 (en) | 2020-12-30 |
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