CN106192018B - 一种锚定巢式多重pcr富集dna目标区域的方法和试剂盒 - Google Patents
一种锚定巢式多重pcr富集dna目标区域的方法和试剂盒 Download PDFInfo
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- CN106192018B CN106192018B CN201510229173.5A CN201510229173A CN106192018B CN 106192018 B CN106192018 B CN 106192018B CN 201510229173 A CN201510229173 A CN 201510229173A CN 106192018 B CN106192018 B CN 106192018B
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Images
Abstract
Description
组分 | 用量(μL) |
片段化DNA | 10 |
T4 DNA聚合酶(NEB) | 5 |
T4 PNK(NEB) | 5 |
dNTP混合物 | 1.6 |
T4 PNK buffer(NEB) | 10 |
ddH<sub>2</sub>O | 余量 |
总量 | 100 |
组分 | 用量(μL) |
末端修复后DNA | 43 |
10×blue buffer(NEB) | 4.6 |
dATP(5mM) | 1 |
Klenow exo-(NEB) | 1 |
ddH<sub>2</sub>O | 余量 |
总量 | 50 |
组分 | 用量(μL) |
末端修复加A后DNA | 33 |
T4 DNA连接酶(NEB) | 1 |
T4 DNA连接酶缓冲液(NEB) | 5 |
接头序列 | 10 |
ddH<sub>2</sub>O | 余量 |
总量 | 50 |
Claims (10)
Priority Applications (1)
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CN201510229173.5A CN106192018B (zh) | 2015-05-07 | 2015-05-07 | 一种锚定巢式多重pcr富集dna目标区域的方法和试剂盒 |
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CN201510229173.5A CN106192018B (zh) | 2015-05-07 | 2015-05-07 | 一种锚定巢式多重pcr富集dna目标区域的方法和试剂盒 |
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CN106192018A CN106192018A (zh) | 2016-12-07 |
CN106192018B true CN106192018B (zh) | 2020-03-24 |
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Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106498504A (zh) * | 2016-12-13 | 2017-03-15 | 上海美迪维康生物科技有限公司 | 基于多重pcr的二代测序建库技术 |
CN106701949B (zh) * | 2016-12-30 | 2019-09-17 | 人和未来生物科技(长沙)有限公司 | 一种减少扩增偏倚的基因突变检测方法和试剂 |
WO2018214036A1 (zh) * | 2017-05-23 | 2018-11-29 | 深圳华大基因股份有限公司 | 基于滚环扩增的基因组目标区域富集方法及其应用 |
CN107190329B (zh) * | 2017-05-27 | 2018-10-19 | 领星生物科技(上海)有限公司 | 基于dna的融合基因定量测序建库、检测方法及其应用 |
CN107164365B (zh) * | 2017-06-16 | 2020-11-27 | 广州普麦健康咨询有限公司 | 应用三代测序技术检测染色体端粒dna全长的方法 |
CN110651050A (zh) * | 2017-07-21 | 2020-01-03 | 深圳华大生命科学研究院 | 用于检测低频突变的靶向富集方法和试剂盒 |
CN107385042B (zh) * | 2017-07-28 | 2021-01-01 | 广州永诺健康科技有限公司 | 一种锚定巢式多重pcr联合高通量测序检测基因融合的多重pcr引物和方法 |
CN108103143B (zh) * | 2017-12-15 | 2021-11-09 | 序康医疗科技(苏州)有限公司 | 一种目标区域多重pcr与快速文库构建的方法 |
CN110628891B (zh) * | 2018-06-25 | 2024-01-09 | 深圳华大智造科技股份有限公司 | 一种对胚胎进行基因异常筛查的方法 |
CN110669823B (zh) * | 2018-07-03 | 2022-05-24 | 中国医学科学院肿瘤医院 | 一种同时检测多种肝癌常见突变的ctDNA文库构建和测序数据分析方法 |
CN109825552B (zh) * | 2019-02-01 | 2022-04-05 | 厦门艾德生物医药科技股份有限公司 | 一种用于对目标区域进行富集的引物及方法 |
CN113811620B (zh) * | 2019-06-26 | 2024-04-09 | 深圳华大智造科技股份有限公司 | 巢式多重pcr高通量测序文库制备方法及试剂盒 |
CN112779320B (zh) * | 2020-12-04 | 2023-07-14 | 深圳市易基因科技有限公司 | 多区域dna甲基化检测探针设计及其检测方法 |
CN115992243B (zh) * | 2022-11-11 | 2024-01-26 | 深圳凯瑞思医疗科技有限公司 | 一种检测卵巢癌的引物组合、试剂盒及文库构建方法 |
CN116083425B (zh) * | 2022-11-11 | 2023-09-29 | 深圳凯瑞思医疗科技有限公司 | 一种检测子宫内膜癌的引物组合、试剂盒及文库构建方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102181533A (zh) * | 2011-03-17 | 2011-09-14 | 北京贝瑞和康生物技术有限公司 | 多样本混合测序方法及试剂盒 |
CN102373288A (zh) * | 2011-11-30 | 2012-03-14 | 盛司潼 | 一种对目标区域进行测序的方法及试剂盒 |
CN102409049A (zh) * | 2010-09-21 | 2012-04-11 | 深圳华大基因科技有限公司 | 一种基于pcr的dna标签文库构建方法 |
CN102586421A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测易瑞沙适用性基因的方法及试剂盒 |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102409049A (zh) * | 2010-09-21 | 2012-04-11 | 深圳华大基因科技有限公司 | 一种基于pcr的dna标签文库构建方法 |
CN102181533A (zh) * | 2011-03-17 | 2011-09-14 | 北京贝瑞和康生物技术有限公司 | 多样本混合测序方法及试剂盒 |
CN102373288A (zh) * | 2011-11-30 | 2012-03-14 | 盛司潼 | 一种对目标区域进行测序的方法及试剂盒 |
CN102586421A (zh) * | 2011-12-27 | 2012-07-18 | 盛司潼 | 一种检测易瑞沙适用性基因的方法及试剂盒 |
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