DE602007011301D1 - Verfahren zum nachweis mutanter allele mit kombination von echtzeit-pcr und rems-pcr - Google Patents

Verfahren zum nachweis mutanter allele mit kombination von echtzeit-pcr und rems-pcr

Info

Publication number
DE602007011301D1
DE602007011301D1 DE602007011301T DE602007011301T DE602007011301D1 DE 602007011301 D1 DE602007011301 D1 DE 602007011301D1 DE 602007011301 T DE602007011301 T DE 602007011301T DE 602007011301 T DE602007011301 T DE 602007011301T DE 602007011301 D1 DE602007011301 D1 DE 602007011301D1
Authority
DE
Germany
Prior art keywords
pcr
rems
procedure
mutant alleles
combining real
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602007011301T
Other languages
English (en)
Inventor
Daniel Adlerstein
Erlet Shehi
Giulia Amicarelli
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Diasorin SpA
Original Assignee
Diasorin SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diasorin SpA filed Critical Diasorin SpA
Publication of DE602007011301D1 publication Critical patent/DE602007011301D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
DE602007011301T 2006-09-26 2007-09-24 Verfahren zum nachweis mutanter allele mit kombination von echtzeit-pcr und rems-pcr Active DE602007011301D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06020128A EP1905842A1 (de) 2006-09-26 2006-09-26 Verfahren zum Nachweis des mutierten Alleles mittels Real Time PCR und REMS-PCR
PCT/EP2007/060116 WO2008037694A1 (en) 2006-09-26 2007-09-24 Method for detection of mutant alleles combining real time pcr and rems-pcr

Publications (1)

Publication Number Publication Date
DE602007011301D1 true DE602007011301D1 (de) 2011-01-27

Family

ID=37687373

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602007011301T Active DE602007011301D1 (de) 2006-09-26 2007-09-24 Verfahren zum nachweis mutanter allele mit kombination von echtzeit-pcr und rems-pcr

Country Status (7)

Country Link
US (1) US8329405B2 (de)
EP (2) EP1905842A1 (de)
AT (1) ATE491811T1 (de)
CA (1) CA2666442A1 (de)
DE (1) DE602007011301D1 (de)
ES (1) ES2354745T3 (de)
WO (1) WO2008037694A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090068643A1 (en) * 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US8999634B2 (en) * 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
JP2012511927A (ja) 2008-12-17 2012-05-31 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
CN103911428B (zh) 2009-03-27 2016-02-24 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
CN102115782B (zh) * 2009-12-30 2014-06-18 北京雅康博生物科技有限公司 用于定量检测K-ras突变的试剂盒
WO2013075079A1 (en) * 2011-11-17 2013-05-23 Rheonix, Inc. System and methods for selective molecular analysis
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
CN103571947B (zh) * 2013-10-09 2015-03-25 武汉百泰基因工程有限公司 一种用于检测kras基因热点突变的试剂盒
US10433030B2 (en) 2014-10-09 2019-10-01 Thuuz, Inc. Generating a customized highlight sequence depicting multiple events
CN106048015A (zh) * 2016-06-07 2016-10-26 中国疾病预防控制中心传染病预防控制所 限制性内切酶介导的依赖于pcr扩增的实时核酸检测技术
CN107937524A (zh) * 2017-12-05 2018-04-20 中源协和基因科技有限公司 人类kras基因突变检测试剂盒及检测方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
WO2006074222A2 (en) * 2005-01-03 2006-07-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primer for nucleic acid detection
EP1969140A2 (de) 2005-11-23 2008-09-17 DiaSorin S.p.A. Reagentien und verfahren zur gleichzeitigen nukleinsäureamplifikation und -detektion

Also Published As

Publication number Publication date
EP1905842A1 (de) 2008-04-02
EP2066804B1 (de) 2010-12-15
CA2666442A1 (en) 2008-04-03
US20100190157A1 (en) 2010-07-29
US8329405B2 (en) 2012-12-11
ATE491811T1 (de) 2011-01-15
EP2066804A1 (de) 2009-06-10
ES2354745T3 (es) 2011-03-17
WO2008037694A1 (en) 2008-04-03

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