WO2012066576A3 - Oligonucleotide primer sequences for detection of leptospira - Google Patents

Oligonucleotide primer sequences for detection of leptospira Download PDF

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Publication number
WO2012066576A3
WO2012066576A3 PCT/IN2011/000797 IN2011000797W WO2012066576A3 WO 2012066576 A3 WO2012066576 A3 WO 2012066576A3 IN 2011000797 W IN2011000797 W IN 2011000797W WO 2012066576 A3 WO2012066576 A3 WO 2012066576A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotide primer
primer sequences
detection
leptospira
present
Prior art date
Application number
PCT/IN2011/000797
Other languages
French (fr)
Other versions
WO2012066576A2 (en
Inventor
Martin Annette
Kashinath Khonde Vinaykumar
Subhash Shukre Kedar
Original Assignee
Godavari Biorefineries Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Godavari Biorefineries Limited filed Critical Godavari Biorefineries Limited
Publication of WO2012066576A2 publication Critical patent/WO2012066576A2/en
Publication of WO2012066576A3 publication Critical patent/WO2012066576A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is directed towards providing oligonucleotide primer sequences for the detection of Leptospira species in biological and environmental samples. The oligonucleotide primer sequences of the present invention are capable of detecting DNA of Leptospira species in a given sample using molecular protocol for example a polymerase chain reaction (PCR) technique. The present invention also provides a method and a kit for detecting leptospiral DNA in a given sample with high specificity.
PCT/IN2011/000797 2010-11-16 2011-11-16 Oligonucleotide primer sequences for detection of leptospira WO2012066576A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN3136/MUM/2010 2010-11-16
IN3136MU2010 2010-11-16

Publications (2)

Publication Number Publication Date
WO2012066576A2 WO2012066576A2 (en) 2012-05-24
WO2012066576A3 true WO2012066576A3 (en) 2012-10-04

Family

ID=46084450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2011/000797 WO2012066576A2 (en) 2010-11-16 2011-11-16 Oligonucleotide primer sequences for detection of leptospira

Country Status (1)

Country Link
WO (1) WO2012066576A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2915997T3 (en) * 2012-10-31 2018-06-29 Hispavista Labs A.I.E. Method for calculating and correcting the angle of attack in a wind turbine farm
CN109182565A (en) * 2018-09-18 2019-01-11 吉林大学 A kind of kit and primer that can detect pathogenic leptospire extensively

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306623B1 (en) * 1998-02-24 2001-10-23 The University Of California Leptospiral major outer membrane protein LipL32

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306623B1 (en) * 1998-02-24 2001-10-23 The University Of California Leptospiral major outer membrane protein LipL32

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHEEMA ET AL.: "Detection of pathogenic leptospires in animals by PCR based on lipL21 and IipL32 genes.", INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY, vol. 45, no. 6, June 2007 (2007-06-01), pages 568 - 573 *
HAAKE ET AL.: "Molecular Evolution and Mosaicism of Leptospiral Outer Membrane Proteins Involves Horizontal DNA Transfer.", J BACTERIOL., vol. 186, no. 9, May 2004 (2004-05-01), pages 2818 - 2828 *
LEVETT ET AL.: "Detection of pathogenic leptospires by real-time quantitative PCR.", J MED MICROBIOL, vol. 54, no. 1, January 2005 (2005-01-01), pages 45 - 49 *
SUZUKI ET AL.: "Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR.", APPL. ENVIRON. MICROBIOL., vol. 62, no. 2, 1 February 1996 (1996-02-01), pages 625 - 630 *
TANSUPHASIRI ET AL.: "Development of a duplex-polymerase chain reaction for rapid detection of pathogenic Leptospira.", SOUTHEAST ASIAN J. TROP. MED. PUBLIC HEALTH, vol. 37, no. 2, March 2006 (2006-03-01), pages 297 - 308 *

Also Published As

Publication number Publication date
WO2012066576A2 (en) 2012-05-24

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