DE602004021352D1 - Stabile lipase-varianten - Google Patents

Stabile lipase-varianten

Info

Publication number
DE602004021352D1
DE602004021352D1 DE602004021352T DE602004021352T DE602004021352D1 DE 602004021352 D1 DE602004021352 D1 DE 602004021352D1 DE 602004021352 T DE602004021352 T DE 602004021352T DE 602004021352 T DE602004021352 T DE 602004021352T DE 602004021352 D1 DE602004021352 D1 DE 602004021352D1
Authority
DE
Germany
Prior art keywords
lipase variants
stable lipase
stable
variants
lipase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004021352T
Other languages
English (en)
Inventor
Nalam Madhusudhana Rao
Priyamvada Acharya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Council of Scientific and Industrial Research CSIR
Original Assignee
Council of Scientific and Industrial Research CSIR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Council of Scientific and Industrial Research CSIR filed Critical Council of Scientific and Industrial Research CSIR
Publication of DE602004021352D1 publication Critical patent/DE602004021352D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE602004021352T 2003-01-30 2004-01-29 Stabile lipase-varianten Expired - Lifetime DE602004021352D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN75DE2003 2003-01-30
PCT/IN2004/000022 WO2004067705A2 (en) 2003-01-30 2004-01-29 Stable lipase variants

Publications (1)

Publication Number Publication Date
DE602004021352D1 true DE602004021352D1 (de) 2009-07-16

Family

ID=32800562

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004021352T Expired - Lifetime DE602004021352D1 (de) 2003-01-30 2004-01-29 Stabile lipase-varianten

Country Status (9)

Country Link
US (1) US7303904B2 (de)
EP (1) EP1590452B1 (de)
JP (1) JP4588695B2 (de)
KR (2) KR20120120461A (de)
CN (1) CN1768135A (de)
CA (1) CA2514914A1 (de)
DE (1) DE602004021352D1 (de)
DK (1) DK1590452T3 (de)
WO (1) WO2004067705A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100417723C (zh) * 2006-03-31 2008-09-10 清华大学 野生型枯草芽胞杆菌脂肪酶a变体及其应用
DE602006017710D1 (en) * 2006-09-14 2010-12-02 Tmo Renewables Ltd Lipase
WO2009008908A2 (en) * 2007-02-12 2009-01-15 Codexis, Inc. Structure-activity relationships
EP2516612A1 (de) * 2009-12-21 2012-10-31 Danisco US Inc. Waschmittelzusammensetzungen mit bacillus subtilis-lipase und verfahren zu ihrer verwendung
CN101857856B (zh) * 2010-05-25 2012-02-01 中国科学院武汉病毒研究所 一种枯草芽孢杆菌脂肪酶及制备方法和应用
CN101838634B (zh) * 2010-05-25 2012-04-25 中国科学院武汉病毒研究所 一种枯草芽孢杆菌脂肪酶及制备方法和应用
CN102653743B (zh) * 2010-08-13 2013-09-18 江南大学 通过定向进化构建的热稳定性提高的脂肪酶突变体
CN102653741B (zh) * 2010-08-13 2013-09-18 江南大学 高热稳定性华根霉脂肪酶
CN102653742B (zh) * 2010-08-13 2014-03-26 江南大学 一种耐高温的华根霉脂肪酶突变体
CN105802937B (zh) * 2016-05-26 2019-10-29 湖南南北旺生物技术有限公司 耐高温脂肪酶的制备方法
CN108220270B (zh) * 2018-01-31 2020-07-07 江南大学 一种催化活性提高的酸性脂肪酶突变体
JP2022545718A (ja) * 2019-08-30 2022-10-28 コデクシス， インコーポレイテッド 操作されたリパーゼ改変体

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
WO2002006457A2 (en) * 2000-07-13 2002-01-24 Maxygen, Inc. Novel lipase genes

Also Published As

Publication number Publication date
EP1590452B1 (de) 2009-06-03
WO2004067705A3 (en) 2004-12-02
CN1768135A (zh) 2006-05-03
DK1590452T3 (da) 2009-08-24
KR20050121667A (ko) 2005-12-27
US20050196834A1 (en) 2005-09-08
EP1590452A2 (de) 2005-11-02
WO2004067705A2 (en) 2004-08-12
JP2007528200A (ja) 2007-10-11
JP4588695B2 (ja) 2010-12-01
KR20120120461A (ko) 2012-11-01
CA2514914A1 (en) 2004-08-12
US7303904B2 (en) 2007-12-04

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Legal Events

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