DE602004004796D1 - Expressionsvektoren, transformierte wirtszellen und fermentationsverfahren zur herstellung rekombinanter polypeptide - Google Patents

Expressionsvektoren, transformierte wirtszellen und fermentationsverfahren zur herstellung rekombinanter polypeptide

Info

Publication number
DE602004004796D1
DE602004004796D1 DE602004004796T DE602004004796T DE602004004796D1 DE 602004004796 D1 DE602004004796 D1 DE 602004004796D1 DE 602004004796 T DE602004004796 T DE 602004004796T DE 602004004796 T DE602004004796 T DE 602004004796T DE 602004004796 D1 DE602004004796 D1 DE 602004004796D1
Authority
DE
Germany
Prior art keywords
preparation
host cells
expression vectors
transformed host
recombinant polypeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602004004796T
Other languages
English (en)
Other versions
DE602004004796T2 (de
Inventor
Joerg Windisch
Kurt Schoergendorfer
Norbert Palma
Franz Knauseder
Hans Boehling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sandoz AG
Original Assignee
Sandoz AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sandoz AG filed Critical Sandoz AG
Publication of DE602004004796D1 publication Critical patent/DE602004004796D1/de
Application granted granted Critical
Publication of DE602004004796T2 publication Critical patent/DE602004004796T2/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE602004004796T 2003-08-13 2004-08-12 Expressionsvektoren, transformierte wirtszellen und fermentationsverfahren zur herstellung rekombinanter polypeptide Active DE602004004796T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US49491403P 2003-08-13 2003-08-13
US494914P 2003-08-13
PCT/EP2004/009067 WO2005017162A2 (en) 2003-08-13 2004-08-12 Expression vectors, transformed host cells and fermentation process for the production of recombinant polypeptides

Publications (2)

Publication Number Publication Date
DE602004004796D1 true DE602004004796D1 (de) 2007-03-29
DE602004004796T2 DE602004004796T2 (de) 2007-12-06

Family

ID=34193254

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004004796T Active DE602004004796T2 (de) 2003-08-13 2004-08-12 Expressionsvektoren, transformierte wirtszellen und fermentationsverfahren zur herstellung rekombinanter polypeptide

Country Status (7)

Country Link
US (1) US7785830B2 (de)
EP (1) EP1656452B1 (de)
JP (1) JP5544063B2 (de)
AT (1) ATE353969T1 (de)
DE (1) DE602004004796T2 (de)
ES (1) ES2281822T3 (de)
WO (1) WO2005017162A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0321100D0 (en) 2003-09-09 2003-10-08 Celltech R&D Ltd Biological products
WO2011123570A2 (en) * 2010-04-01 2011-10-06 Pfenex Inc. Methods for g-csf production in a pseudomonas host cell
AR090340A1 (es) * 2012-03-27 2014-11-05 Genentech Inc Operaciones de cosecha mejoradas para proteinas recombinadas
CN114561395B (zh) * 2022-03-30 2023-07-28 四川大学 无融合标签的rhIL-11及其突变体的可溶表达及高效纯化方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4680262A (en) 1984-10-05 1987-07-14 Genentech, Inc. Periplasmic protein recovery
GB8715428D0 (en) 1987-07-01 1987-08-26 Univ Dundee Organic compounds
WO1993006217A1 (en) 1991-09-19 1993-04-01 Genentech, Inc. EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES
EP0626448A3 (de) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Herstellung und Reinigung von alpha-Interferon
US6160089A (en) 1998-07-08 2000-12-12 Mitsui Chemicals, Inc. Method for secretory production of human growth hormone
KR100360594B1 (ko) * 2000-01-19 2002-11-13 한미약품공업 주식회사 인간 인터페론 알파의 발현 분비벡터 및 이를 이용한인터페론 알파의 생산 방법
GB0013810D0 (en) 2000-06-06 2000-07-26 Celltech Chiroscience Ltd Biological products
WO2003004599A2 (en) * 2001-07-02 2003-01-16 Insight Biopharmaceuticals Ltd. Methods of making and using expression constructs for the expression of recombinant proteins

Also Published As

Publication number Publication date
ATE353969T1 (de) 2007-03-15
EP1656452B1 (de) 2007-02-14
US7785830B2 (en) 2010-08-31
WO2005017162A2 (en) 2005-02-24
JP2007501623A (ja) 2007-02-01
DE602004004796T2 (de) 2007-12-06
ES2281822T3 (es) 2007-10-01
JP5544063B2 (ja) 2014-07-09
EP1656452A2 (de) 2006-05-17
WO2005017162A3 (en) 2005-05-19
US20060228783A1 (en) 2006-10-12

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: KROHER, STROBEL RECHTS- UND PATENTANWAELTE, 80336

8328 Change in the person/name/address of the agent

Representative=s name: DR. SCHOEN & PARTNER, 80336 MUENCHEN