DE60140996D1 - Intein enthaltende multigen-expressionskonstrukte - Google Patents
Intein enthaltende multigen-expressionskonstrukteInfo
- Publication number
- DE60140996D1 DE60140996D1 DE60140996T DE60140996T DE60140996D1 DE 60140996 D1 DE60140996 D1 DE 60140996D1 DE 60140996 T DE60140996 T DE 60140996T DE 60140996 T DE60140996 T DE 60140996T DE 60140996 D1 DE60140996 D1 DE 60140996D1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- plants
- constructs
- fused
- splicing unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18173900P | 2000-02-11 | 2000-02-11 | |
PCT/US2001/004254 WO2001059091A2 (en) | 2000-02-11 | 2001-02-09 | Multi-gene expression constructs containing modified inteins |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60140996D1 true DE60140996D1 (de) | 2010-02-25 |
Family
ID=22665581
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60140996T Expired - Lifetime DE60140996D1 (de) | 2000-02-11 | 2001-02-09 | Intein enthaltende multigen-expressionskonstrukte |
Country Status (7)
Country | Link |
---|---|
US (3) | US7026526B2 (de) |
EP (3) | EP1255846B1 (de) |
AT (1) | ATE454456T1 (de) |
AU (1) | AU2001236839A1 (de) |
DE (1) | DE60140996D1 (de) |
ES (1) | ES2336641T3 (de) |
WO (1) | WO2001059091A2 (de) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7026526B2 (en) * | 2000-02-11 | 2006-04-11 | Metabolix, Inc. | Multi-gene expression constructs containing modified inteins |
WO2001088168A2 (en) * | 2000-05-12 | 2001-11-22 | Odyssey Pharmaceuticals, Inc. | Mapping molecular interactions in plants with protein fragments complementation assays |
CN101993907B (zh) * | 2002-01-08 | 2018-12-28 | 迈克尔·R·拉尔布 | 表达civps或内含肽修饰蛋白的转基因植物及其制备方法 |
JP4518741B2 (ja) * | 2002-04-29 | 2010-08-04 | アイコン・ジェネティクス・ゲーエムベーハー | 環境に安全なトランスジェニック生物を産生する方法 |
EP1509610B1 (de) * | 2002-05-31 | 2012-05-23 | Bayer CropScience N.V. | Transgene-pflanzen mit kontrollierter verteilung eines merkmals zu den nachkommen |
CA2762011C (en) | 2004-04-09 | 2019-05-07 | Monsanto Technology Llc | Compositions and methods for control of insect infestations in plants |
CA2600882C (en) | 2005-03-16 | 2013-02-26 | Metabolix, Inc. | Chemically inducible expression of biosynthetic pathways |
JP2009508470A (ja) * | 2005-07-21 | 2009-03-05 | アボット・ラボラトリーズ | Sorf構築物並びにポリタンパク質、プロタンパク質及びタンパク質分解による方法を含む複数の遺伝子発現 |
US9150877B2 (en) * | 2007-09-27 | 2015-10-06 | Dow Agrosciences Llc | Construct and method for expressing transgenes using a Brassica bidirectional constitutive promoter |
WO2011011693A1 (en) | 2009-07-23 | 2011-01-27 | Chromatin, Inc. | Sorghum centromere sequences and minichromosomes |
EP2478105A1 (de) | 2009-09-15 | 2012-07-25 | Metabolix, Inc. | Erzeugung von ölsamen zur hohen polyhydroxybuttersäureproduktion |
KR20120091251A (ko) * | 2009-10-30 | 2012-08-17 | 아보트 러보러터리즈 | Sorf 작제물 및 다중 유전자 발현 |
US9464333B2 (en) | 2009-11-06 | 2016-10-11 | Agrivida, Inc. | Intein-modified enzymes, their production and industrial applications |
US8420387B2 (en) | 2009-11-06 | 2013-04-16 | Agrivida, Inc. | Intein-modified enzymes, their production and industrial applications |
US10407742B2 (en) | 2009-11-06 | 2019-09-10 | Agrivida, Inc. | Intein-modified enzymes, their production and industrial applications |
US8609967B2 (en) * | 2010-01-14 | 2013-12-17 | Kmc Music, Inc. | Top-tuning system for hand percussion instrument |
US9598700B2 (en) | 2010-06-25 | 2017-03-21 | Agrivida, Inc. | Methods and compositions for processing biomass with elevated levels of starch |
US10443068B2 (en) | 2010-06-25 | 2019-10-15 | Agrivida, Inc. | Plants with engineered endogenous genes |
CN106244622B (zh) | 2011-03-07 | 2020-06-12 | 谷万达公司 | 在工程植物中表达多种蛋白的表达盒和表达载体及制备工程植物和生产可溶性糖的方法 |
USD831312S1 (en) | 2014-11-14 | 2018-10-23 | Gavrieli Brands LLC | Dual-sole shoe with blue outpatch sole and contrasting upper |
US8945876B2 (en) | 2011-11-23 | 2015-02-03 | University Of Hawaii | Auto-processing domains for polypeptide expression |
RU2619178C2 (ru) | 2012-06-07 | 2017-05-12 | ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи | Конструкт и способ для экспрессии трансгенов с использованием двунаправленного конститутивного промотора brassica |
US9963707B2 (en) | 2012-10-03 | 2018-05-08 | Agrivida, Inc. | Multiprotein expression cassettes |
RU2601126C2 (ru) * | 2014-09-17 | 2016-10-27 | Федеральное государственное бюджетное учреждение "Государственный научный центр Институт иммунологии" Федерального медико-биологического агентства России | Способ получения биологически активных пептидов |
EP3660053A1 (de) * | 2018-11-27 | 2020-06-03 | Fraunhofer Gesellschaft zur Förderung der Angewand | Multiproteinexpressionssysteme zur schädlingsbekämpfung |
Family Cites Families (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5352605A (en) | 1983-01-17 | 1994-10-04 | Monsanto Company | Chimeric genes for transforming plant cells using viral promoters |
US5034322A (en) | 1983-01-17 | 1991-07-23 | Monsanto Company | Chimeric genes suitable for expression in plant cells |
US5231019A (en) | 1984-05-11 | 1993-07-27 | Ciba-Geigy Corporation | Transformation of hereditary material of plants |
US5668298A (en) | 1984-12-24 | 1997-09-16 | Eli Lilly And Company | Selectable marker for development of vectors and transformation systems in plants |
US5420034A (en) | 1986-07-31 | 1995-05-30 | Calgene, Inc. | Seed-specific transcriptional regulation |
US5188958A (en) | 1986-05-29 | 1993-02-23 | Calgene, Inc. | Transformation and foreign gene expression in brassica species |
US5024944A (en) | 1986-08-04 | 1991-06-18 | Lubrizol Genetics, Inc. | Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species |
US5276268A (en) | 1986-08-23 | 1994-01-04 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5268463A (en) | 1986-11-11 | 1993-12-07 | Jefferson Richard A | Plant promoter α-glucuronidase gene construct |
US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
US5030572A (en) | 1987-04-01 | 1991-07-09 | Lubrizol Genetics, Inc. | Sunflower regeneration from cotyledons |
US5480794A (en) * | 1987-06-29 | 1996-01-02 | Massachusetts Institute Of Technology And Metabolix, Inc. | Overproduction and purification of soluble PHA synthase |
JPH04501201A (ja) | 1987-12-21 | 1992-03-05 | ジ・アップジョン・カンパニー | 発芽植物種子類のアグロバクテリウム媒介形質転換 |
US5614395A (en) | 1988-03-08 | 1997-03-25 | Ciba-Geigy Corporation | Chemically regulatable and anti-pathogenic DNA sequences and uses thereof |
US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
US5364780A (en) | 1989-03-17 | 1994-11-15 | E. I. Du Pont De Nemours And Company | External regulation of gene expression by inducible promoters |
US5629183A (en) | 1989-05-08 | 1997-05-13 | The United States Of America As Represented By The Secretary Of Agriculture | Plant transformation by gene transfer into pollen |
US5302523A (en) | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
DE69032713T2 (de) | 1989-07-10 | 1999-04-08 | Massachusetts Inst Technology | Verfahren zur Herstellung von Polyester-Biopolymeren |
US5322783A (en) | 1989-10-17 | 1994-06-21 | Pioneer Hi-Bred International, Inc. | Soybean transformation by microparticle bombardment |
US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
US5519164A (en) | 1990-02-01 | 1996-05-21 | Hoechst Aktiengesellschaft | Expression of a multigene RNA having self-splicing activity |
NZ239977A (en) | 1990-11-14 | 1993-08-26 | Pioneer Hi Bred Int | Transforming plants by the use of agrobacterium |
US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
WO1993020216A1 (en) | 1991-02-22 | 1993-10-14 | University Technologies International, Inc. | Oil-body protein cis-elements as regulatory signals |
US5650554A (en) | 1991-02-22 | 1997-07-22 | Sembiosys Genetics Inc. | Oil-body proteins as carriers of high-value peptides in plants |
DK152291D0 (da) | 1991-08-28 | 1991-08-28 | Danisco | Fremgangsmaade og kemiske forbindelser |
GB9304200D0 (en) | 1993-03-02 | 1993-04-21 | Sandoz Ltd | Improvements in or relating to organic compounds |
ATE398679T1 (de) | 1992-07-07 | 2008-07-15 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
US5527695A (en) | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
WO1995021249A1 (en) | 1994-02-03 | 1995-08-10 | The Scripps Research Institute | A cassette to accumulate multiple proteins through synthesis of a self-processing polypeptide |
FR2717187B1 (fr) | 1994-03-10 | 1996-05-31 | Transgene Sa | Usage combiné de deux cassettes d'expression pour la production d'une protéine d'intérêt. |
DE4433134A1 (de) | 1994-09-16 | 1996-03-21 | Buck Chem Tech Werke | Verfahren zur Herstellung von Polyhydroxyfettsäuren sowie rekombinanter Bakterienstämme zur Durchführung des Verfahrens |
US5958745A (en) | 1996-03-13 | 1999-09-28 | Monsanto Company | Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants |
US5750848A (en) | 1996-08-13 | 1998-05-12 | Monsanto Company | DNA sequence useful for the production of polyhydroxyalkanoates |
WO1998036078A1 (en) | 1997-02-13 | 1998-08-20 | James Madison University | Methods of making polyhydroxyalkanoates comprising 4-hydroxybutyrate monomer units |
CA2303070C (en) | 1997-09-19 | 2011-03-15 | Metabolix, Inc. | Biological systems for manufacture of polyhydroxylalkanoate polymers containing 4-hydroxyacids |
US6858775B1 (en) * | 1999-05-24 | 2005-02-22 | New England Biolabs, Inc. | Method for generating split, non-transferable genes that are able to express an active protein product |
US7026526B2 (en) * | 2000-02-11 | 2006-04-11 | Metabolix, Inc. | Multi-gene expression constructs containing modified inteins |
-
2001
- 2001-02-09 US US09/779,957 patent/US7026526B2/en not_active Expired - Fee Related
- 2001-02-09 AT AT01909045T patent/ATE454456T1/de not_active IP Right Cessation
- 2001-02-09 EP EP01909045A patent/EP1255846B1/de not_active Expired - Lifetime
- 2001-02-09 EP EP09075516.6A patent/EP2159288A3/de not_active Withdrawn
- 2001-02-09 DE DE60140996T patent/DE60140996D1/de not_active Expired - Lifetime
- 2001-02-09 AU AU2001236839A patent/AU2001236839A1/en not_active Abandoned
- 2001-02-09 ES ES01909045T patent/ES2336641T3/es not_active Expired - Lifetime
- 2001-02-09 EP EP09075515.8A patent/EP2159287A3/de not_active Withdrawn
- 2001-02-09 WO PCT/US2001/004254 patent/WO2001059091A2/en active Application Filing
-
2005
- 2005-06-08 US US11/147,546 patent/US7741530B2/en not_active Expired - Fee Related
-
2010
- 2010-04-21 US US12/764,516 patent/US8293971B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP2159287A3 (de) | 2014-02-12 |
US7741530B2 (en) | 2010-06-22 |
EP2159287A2 (de) | 2010-03-03 |
EP1255846B1 (de) | 2010-01-06 |
US20020129400A1 (en) | 2002-09-12 |
US20050283850A1 (en) | 2005-12-22 |
US8293971B2 (en) | 2012-10-23 |
EP2159288A3 (de) | 2014-02-12 |
AU2001236839A1 (en) | 2001-08-20 |
US20100251418A1 (en) | 2010-09-30 |
US7026526B2 (en) | 2006-04-11 |
ES2336641T3 (es) | 2010-04-15 |
WO2001059091A2 (en) | 2001-08-16 |
ATE454456T1 (de) | 2010-01-15 |
EP2159288A2 (de) | 2010-03-03 |
EP1255846A2 (de) | 2002-11-13 |
WO2001059091A3 (en) | 2001-12-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |