DE60138344D1 - Verfahren zur Mutagenese - Google Patents
Verfahren zur MutageneseInfo
- Publication number
- DE60138344D1 DE60138344D1 DE60138344T DE60138344T DE60138344D1 DE 60138344 D1 DE60138344 D1 DE 60138344D1 DE 60138344 T DE60138344 T DE 60138344T DE 60138344 T DE60138344 T DE 60138344T DE 60138344 D1 DE60138344 D1 DE 60138344D1
- Authority
- DE
- Germany
- Prior art keywords
- mutagenesis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title 1
- 238000002703 mutagenesis Methods 0.000 title 1
- 231100000350 mutagenesis Toxicity 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000237166A JP3859947B2 (ja) | 2000-08-04 | 2000-08-04 | 突然変異導入方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60138344D1 true DE60138344D1 (de) | 2009-05-28 |
Family
ID=18729074
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60138344T Expired - Lifetime DE60138344D1 (de) | 2000-08-04 | 2001-08-03 | Verfahren zur Mutagenese |
Country Status (4)
Country | Link |
---|---|
US (1) | US6673610B2 (de) |
EP (1) | EP1178109B1 (de) |
JP (1) | JP3859947B2 (de) |
DE (1) | DE60138344D1 (de) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2823219B1 (fr) * | 2001-04-10 | 2003-07-04 | Pasteur Institut | Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie |
ATE432981T1 (de) * | 2001-07-26 | 2009-06-15 | Stratagene California | Multi-stellen-mutagenese |
JP4431778B2 (ja) * | 2002-07-09 | 2010-03-17 | 独立行政法人産業技術総合研究所 | 組み換えプラスミドの作成方法 |
SI1844144T1 (sl) * | 2005-01-10 | 2010-07-30 | Medimmune Ltd | Postopek mutageneze |
US20100267147A1 (en) * | 2007-04-25 | 2010-10-21 | GM Biosciences, Inc. | Site-directed mutagenesis in circular methylated dna |
EP2183378A4 (de) | 2007-07-31 | 2010-09-22 | Verenium Corp | Massgeschneiderte kombinatorische mehrfachstellen-konstruktion |
ITTO20070687A1 (it) * | 2007-10-01 | 2009-04-02 | Consiglio Nazionale Ricerche | Nuovo mutante di gfp sensibile al ph e alla concentrazione di anioni, proteina chimerica comprendente tale mutante e procedimento di determinazione combinata del ph e della concentrazione di anioni |
CN101210240B (zh) * | 2007-12-25 | 2011-11-16 | 天津大学 | 大引物pcr进行定点突变的方法 |
US9476079B2 (en) | 2012-01-20 | 2016-10-25 | Kennesaw State University Research And Services Foundation, Inc. | Methods to mutate circular deoxyribonucleotides |
WO2013188578A1 (en) * | 2012-06-12 | 2013-12-19 | The Johns Hopkins University | Efficient, expansive, user-defined dna mutagenesis |
WO2014071182A1 (en) | 2012-11-01 | 2014-05-08 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
RU2769873C2 (ru) | 2015-07-13 | 2022-04-07 | Пивот Байо, Инк. | Способы и композиции для улучшения признаков растений |
JP2018537119A (ja) | 2015-10-05 | 2018-12-20 | マサチューセッツ インスティテュート オブ テクノロジー | リファクターされたnifクラスターを使用する窒素固定 |
EP3568385A4 (de) | 2017-01-12 | 2021-03-03 | Pivot Bio, Inc. | Verfahren und zusammensetzungen zur verbesserung von pflanzeneigenschaften |
JP7420712B2 (ja) | 2017-10-25 | 2024-01-23 | ピボット バイオ, インコーポレイテッド | 窒素を固定する操作された微生物を改良するための方法および組成物 |
BR112020026771A2 (pt) | 2018-06-27 | 2021-03-30 | Pivot Bio, Inc. | Composições agrícolas que compreendem micróbios de fixação de nitrogênio remodelados |
CN113825393A (zh) | 2018-12-21 | 2021-12-21 | 皮沃特生物股份有限公司 | 改善植物性状的方法、组合物和培养基 |
US20200208160A1 (en) | 2018-12-28 | 2020-07-02 | Braskem S.A. | Modulation of carbon flux through the meg and c3 pathways for the improved production of monoethylene glycol and c3 compounds |
AR118793A1 (es) | 2019-04-24 | 2021-11-03 | Pivot Bio Inc | Dianas genéticas para dirigir la fijación de nitrógeno para mejorar las características de plantas |
WO2021221690A1 (en) | 2020-05-01 | 2021-11-04 | Pivot Bio, Inc. | Modified bacterial strains for improved fixation of nitrogen |
WO2021222567A2 (en) | 2020-05-01 | 2021-11-04 | Pivot Bio, Inc. | Modified bacterial strains for improved fixation of nitrogen |
CA3172637A1 (en) | 2020-05-01 | 2021-11-04 | Pivot Bio, Inc. | Measurement of nitrogen fixation and incorporation |
BR112022022714A2 (pt) | 2020-05-13 | 2023-03-28 | Pivot Bio Inc | Bactéria diazotrófica gram-positiva geneticamente modificada, composição microbiana, método para fornecer nitrogênio fixo a uma planta, gene glnr, proteína glnr, e, método para identificar reguladores de um operon nif |
AU2022301301A1 (en) | 2021-07-02 | 2023-12-14 | Pivot Bio, Inc. | Genetically-engineered bacterial strains for improved fixation of nitrogen |
CN116083544A (zh) * | 2022-03-18 | 2023-05-09 | 华中科技大学 | 一种m6A依赖型限制性内切酶、其突变体及其应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69233035D1 (de) * | 1991-12-24 | 2003-06-05 | Harvard College | Gezielte punkt-mutagenese von dna |
US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US5939250A (en) * | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US5789166A (en) * | 1995-12-08 | 1998-08-04 | Stratagene | Circular site-directed mutagenesis |
-
2000
- 2000-08-04 JP JP2000237166A patent/JP3859947B2/ja not_active Expired - Fee Related
-
2001
- 2001-08-02 US US09/920,922 patent/US6673610B2/en not_active Expired - Fee Related
- 2001-08-03 EP EP01306650A patent/EP1178109B1/de not_active Expired - Lifetime
- 2001-08-03 DE DE60138344T patent/DE60138344D1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP3859947B2 (ja) | 2006-12-20 |
US20020083488A1 (en) | 2002-06-27 |
EP1178109A1 (de) | 2002-02-06 |
US6673610B2 (en) | 2004-01-06 |
EP1178109B1 (de) | 2009-04-15 |
JP2002045182A (ja) | 2002-02-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |