BR112022022714A2 - Bactéria diazotrófica gram-positiva geneticamente modificada, composição microbiana, método para fornecer nitrogênio fixo a uma planta, gene glnr, proteína glnr, e, método para identificar reguladores de um operon nif - Google Patents

Bactéria diazotrófica gram-positiva geneticamente modificada, composição microbiana, método para fornecer nitrogênio fixo a uma planta, gene glnr, proteína glnr, e, método para identificar reguladores de um operon nif

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Publication number
BR112022022714A2
BR112022022714A2 BR112022022714A BR112022022714A BR112022022714A2 BR 112022022714 A2 BR112022022714 A2 BR 112022022714A2 BR 112022022714 A BR112022022714 A BR 112022022714A BR 112022022714 A BR112022022714 A BR 112022022714A BR 112022022714 A2 BR112022022714 A2 BR 112022022714A2
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Brazil
Prior art keywords
glnr
positive
protein
plant
genetically modified
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BR112022022714A
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English (en)
Inventor
Ozaydin Eskiyenenturk Bilge
Ryu Min-Hyung
JOHNSON Jenny
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Pivot Bio Inc
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Publication date
Application filed by Pivot Bio Inc filed Critical Pivot Bio Inc
Publication of BR112022022714A2 publication Critical patent/BR112022022714A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0095—Oxidoreductases (1.) acting on iron-sulfur proteins as donor (1.18)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/25—Paenibacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y118/00—Oxidoreductases acting on iron-sulfur proteins as donors (1.18)
    • C12Y118/06—Oxidoreductases acting on iron-sulfur proteins as donors (1.18) with dinitrogen as acceptor (1.18.6)
    • C12Y118/06001—Nitrogenase (1.18.6.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
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    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/12—Bacillus polymyxa ; Paenibacillus polymyxa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pest Control & Pesticides (AREA)
  • Molecular Biology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

BACTÉRIA DIAZOTRÓFICA GRAM-POSITIVA GENETICAMENTE MODIFICADA, COMPOSIÇÃO MICROBIANA, MÉTODO PARA FORNECER NITROGÊNIO FIXO A UMA PLANTA, GENE GLNR, PROTEÍNA GLNR, E, MÉTODO PARA IDENTIFICAR REGULADORES DE UM OPERON NIF. A presente revelação fornece micróbios gram-positivos geneticamente modificados que são capazes de fixar nitrogênio atmosférico e liberá-lo às plantas de uma maneira direcionada, eficiente e ambientalmente sustentável. A utilização dos produtos microbianos aqui apresentados permitirá que os agricultores alcancem rendimentos de culturas mais produtivos e previsíveis sem a degradação de nutrientes, lixiviação ou escoamento tóxico associados ao fertilizante nitrogenado tradicional, derivado sinteticamente.
BR112022022714A 2020-05-13 2021-05-11 Bactéria diazotrófica gram-positiva geneticamente modificada, composição microbiana, método para fornecer nitrogênio fixo a uma planta, gene glnr, proteína glnr, e, método para identificar reguladores de um operon nif BR112022022714A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063024208P 2020-05-13 2020-05-13
PCT/US2021/031808 WO2021231449A2 (en) 2020-05-13 2021-05-11 De-repression of nitrogen fixation in gram-positive microorganisms

Publications (1)

Publication Number Publication Date
BR112022022714A2 true BR112022022714A2 (pt) 2023-03-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022022714A BR112022022714A2 (pt) 2020-05-13 2021-05-11 Bactéria diazotrófica gram-positiva geneticamente modificada, composição microbiana, método para fornecer nitrogênio fixo a uma planta, gene glnr, proteína glnr, e, método para identificar reguladores de um operon nif

Country Status (7)

Country Link
US (1) US20230295559A1 (pt)
EP (1) EP4149245A2 (pt)
AR (1) AR122091A1 (pt)
BR (1) BR112022022714A2 (pt)
CA (1) CA3172323A1 (pt)
UY (1) UY39215A (pt)
WO (1) WO2021231449A2 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3701040A4 (en) 2017-10-25 2021-08-25 Pivot Bio, Inc. METHODS AND COMPOSITIONS FOR IMPROVING GENETICALLY MODIFIED MICROBES THAT BIND NITROGEN
EP3814302A4 (en) 2018-06-27 2022-06-29 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
CN113980863B (zh) * 2021-11-26 2022-05-31 南京工业大学 一株暹罗芽孢杆菌及其应用

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US5071743A (en) 1989-10-27 1991-12-10 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Process for conducting site-directed mutagenesis
ATE239089T1 (de) 1991-12-24 2003-05-15 Harvard College Gezielte punkt-mutagenese von dna
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US5789166A (en) 1995-12-08 1998-08-04 Stratagene Circular site-directed mutagenesis
US5780270A (en) 1996-07-17 1998-07-14 Promega Corporation Site-specific mutagenesis and mutant selection utilizing antibiotic-resistant markers encoding gene products having altered substrate specificity
US6033861A (en) 1997-11-19 2000-03-07 Incyte Genetics, Inc. Methods for obtaining nucleic acid containing a mutation
JP3859947B2 (ja) 2000-08-04 2006-12-20 独立行政法人理化学研究所 突然変異導入方法
US20050266541A1 (en) 2002-11-04 2005-12-01 Harrison F. Dillon Methods and compositions for evolving microbial hydrogen production
US7485451B2 (en) 2004-11-18 2009-02-03 Regents Of The University Of California Storage stable compositions of biological materials
US20100267147A1 (en) 2007-04-25 2010-10-21 GM Biosciences, Inc. Site-directed mutagenesis in circular methylated dna
US9228240B2 (en) 2010-06-03 2016-01-05 California Institute Of Technology Methods for detecting and quantifying viable bacterial endo-spores
PL2721153T3 (pl) 2011-06-16 2020-03-31 The Regents Of The University Of California Syntetyczne klastry genów
US20140189896A1 (en) 2012-12-12 2014-07-03 Feng Zhang Crispr-cas component systems, methods and compositions for sequence manipulation
CA2906970C (en) 2013-03-21 2021-05-18 Ospedale San Raffaele Srl Targeted disruption of t cell receptor genes using engineered zinc finger protein nucleases
CN105849104B (zh) 2013-10-25 2020-05-22 健康农业公司 独脚金内酯制剂及其用途
AU2018354338B2 (en) * 2017-10-25 2023-10-26 Pivot Bio, Inc. Gene targets for nitrogen fixation targeting for improving plant traits

Also Published As

Publication number Publication date
AR122091A1 (es) 2022-08-10
UY39215A (es) 2021-11-30
WO2021231449A2 (en) 2021-11-18
WO2021231449A3 (en) 2022-03-31
US20230295559A1 (en) 2023-09-21
EP4149245A2 (en) 2023-03-22
CA3172323A1 (en) 2021-11-18

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