DE60121219D1 - Verfahren zum Nachweis von Homo- oder Heterozygotie eines Organismus unter Verwendung markerter Primer und RFLP - Google Patents

Verfahren zum Nachweis von Homo- oder Heterozygotie eines Organismus unter Verwendung markerter Primer und RFLP

Info

Publication number
DE60121219D1
DE60121219D1 DE60121219T DE60121219T DE60121219D1 DE 60121219 D1 DE60121219 D1 DE 60121219D1 DE 60121219 T DE60121219 T DE 60121219T DE 60121219 T DE60121219 T DE 60121219T DE 60121219 D1 DE60121219 D1 DE 60121219D1
Authority
DE
Germany
Prior art keywords
organism
heterozygosity
rflp
labels
labeled primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60121219T
Other languages
English (en)
Other versions
DE60121219T2 (de
Inventor
Kirby Siemering
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Murdoch Childrens Research Institute
Original Assignee
Murdoch Childrens Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Murdoch Childrens Research Institute filed Critical Murdoch Childrens Research Institute
Application granted granted Critical
Publication of DE60121219D1 publication Critical patent/DE60121219D1/de
Publication of DE60121219T2 publication Critical patent/DE60121219T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
DE60121219T 2000-12-20 2001-12-20 Verfahren zum Nachweis von Homo-oder Heterozygotie eines Organismus unter Verwendung markerter Primer und RFLP Expired - Lifetime DE60121219T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPR221400 2000-12-20
AUPR2214A AUPR221400A0 (en) 2000-12-20 2000-12-20 Diagnostic assay
PCT/AU2001/001643 WO2002050305A1 (en) 2000-12-20 2001-12-20 Method for detecting whether an organism is homozygous or heterozygous using labelled primers and rflp

Publications (2)

Publication Number Publication Date
DE60121219D1 true DE60121219D1 (de) 2006-08-10
DE60121219T2 DE60121219T2 (de) 2007-06-06

Family

ID=3826248

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60121219T Expired - Lifetime DE60121219T2 (de) 2000-12-20 2001-12-20 Verfahren zum Nachweis von Homo-oder Heterozygotie eines Organismus unter Verwendung markerter Primer und RFLP

Country Status (9)

Country Link
US (1) US7459271B2 (de)
EP (1) EP1354059B1 (de)
JP (3) JP2004523224A (de)
AT (1) ATE331813T1 (de)
AU (1) AUPR221400A0 (de)
CA (1) CA2431882A1 (de)
DE (1) DE60121219T2 (de)
NZ (1) NZ526427A (de)
WO (1) WO2002050305A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003283054B2 (en) * 2002-11-18 2009-02-05 Murdoch Childrens Research Institute Genotyping of deafness by oligonucleotide microarray analysis
AU2002952702A0 (en) * 2002-11-18 2002-11-28 Murdoch Childrens Research Institute A diagnostic assay
WO2004050916A1 (en) * 2002-12-02 2004-06-17 Solexa Limited Recovery of original template
US8275554B2 (en) * 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
CA2510166A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US20040166495A1 (en) * 2003-02-24 2004-08-26 Greinwald John H. Microarray-based diagnosis of pediatric hearing impairment-construction of a deafness gene chip
WO2005094279A2 (en) * 2004-03-25 2005-10-13 The Rothberg Institute For Childhood Diseases Immortalized human tuberous sclerosis null angiomyolipoma cell and method of use thereof
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US9029090B2 (en) * 2010-03-01 2015-05-12 Institute of Animal Science Chinese Academy of Agricultural Sciences Method for auxiliary identification of inbred line of wuzhishan miniature pig and its special primer
GB201418892D0 (en) * 2014-10-23 2014-12-10 Proqr Therapeutics B V DNA editing
CN114438224A (zh) * 2022-01-27 2022-05-06 江苏省家禽科学研究所 一种鸭蛋壳性状相关基因型的分子标记、引物及其检测方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025563A1 (en) 1992-06-17 1993-12-23 City Of Hope A method of detecting and discriminating between nucleic acid sequences
AU2187295A (en) * 1994-03-18 1995-10-09 General Hospital Corporation, The Cleaved amplified rflp detection methods
US6110709A (en) * 1994-03-18 2000-08-29 The General Hospital Corporation Cleaved amplified modified polymorphic sequence detection methods
FR2718461B1 (fr) 1994-04-07 1996-05-15 Cis Bio Int Procédé de détection d'un site de restriction dans une séquence d'ADN.
EP0948645A4 (de) 1996-09-18 2002-11-05 Gen Hospital Corp Erkennungsverfahren durch restriktionsfragmentlängenpolymorphismen (rflp)
WO1998053098A1 (de) * 1997-05-21 1998-11-26 GESELLSCHAFT FüR BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) Verfahren und kit zur detektion von mutationen in dna's mit hilfe von restriktionsenzymen
ATE317024T1 (de) * 1999-12-29 2006-02-15 Keygene Nv Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden

Also Published As

Publication number Publication date
EP1354059B1 (de) 2006-06-28
EP1354059A4 (de) 2004-07-07
AUPR221400A0 (en) 2001-01-25
DE60121219T2 (de) 2007-06-06
US20040132034A1 (en) 2004-07-08
JP2009034111A (ja) 2009-02-19
EP1354059A1 (de) 2003-10-22
ATE331813T1 (de) 2006-07-15
US7459271B2 (en) 2008-12-02
JP2004523224A (ja) 2004-08-05
WO2002050305A1 (en) 2002-06-27
JP2010022383A (ja) 2010-02-04
NZ526427A (en) 2005-07-29
CA2431882A1 (en) 2002-06-27

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