DE60115405D1 - Verbesserungen in bezug auf ribosom-komplexe als selektionspartikel - Google Patents

Verbesserungen in bezug auf ribosom-komplexe als selektionspartikel

Info

Publication number
DE60115405D1
DE60115405D1 DE60115405T DE60115405T DE60115405D1 DE 60115405 D1 DE60115405 D1 DE 60115405D1 DE 60115405 T DE60115405 T DE 60115405T DE 60115405 T DE60115405 T DE 60115405T DE 60115405 D1 DE60115405 D1 DE 60115405D1
Authority
DE
Germany
Prior art keywords
specific binding
selection
oxidised
optionally
improvements relating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60115405T
Other languages
English (en)
Other versions
DE60115405T2 (de
Inventor
Jane Osbourn
Thor Holet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MedImmune Ltd
Original Assignee
Cambridge Antibody Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Antibody Technology Ltd filed Critical Cambridge Antibody Technology Ltd
Publication of DE60115405D1 publication Critical patent/DE60115405D1/de
Application granted granted Critical
Publication of DE60115405T2 publication Critical patent/DE60115405T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Ink Jet Recording Methods And Recording Media Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60115405T 2000-03-31 2001-03-26 Verbesserungen in bezug auf ribosom-komplexe als selektionspartikel Expired - Lifetime DE60115405T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19380200P 2000-03-31 2000-03-31
US193802P 2000-03-31
PCT/GB2001/001319 WO2001075097A2 (en) 2000-03-31 2001-03-26 Improvements to ribosome display

Publications (2)

Publication Number Publication Date
DE60115405D1 true DE60115405D1 (de) 2006-01-05
DE60115405T2 DE60115405T2 (de) 2006-08-03

Family

ID=22715061

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60115405T Expired - Lifetime DE60115405T2 (de) 2000-03-31 2001-03-26 Verbesserungen in bezug auf ribosom-komplexe als selektionspartikel

Country Status (11)

Country Link
US (2) US7074557B2 (de)
EP (1) EP1268766B1 (de)
JP (1) JP2003529369A (de)
AT (1) ATE311444T1 (de)
AU (2) AU2001239444B2 (de)
CA (1) CA2402452A1 (de)
DE (1) DE60115405T2 (de)
DK (1) DK1268766T3 (de)
ES (1) ES2253358T3 (de)
GB (1) GB2365429B (de)
WO (1) WO2001075097A2 (de)

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CA2291721C (en) 1997-05-28 2011-08-09 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
EP1664343B1 (de) * 2003-09-09 2014-05-07 Integrigen, Inc. Verfahren und zusammensetzungen zur erzeugung menschlicher keimlinien-antikörpergene
US20070111237A1 (en) * 2005-09-14 2007-05-17 Maurizio Zanetti Process for identifying antigenized antibodies using ribosome cell free expression system
US7749957B2 (en) 2006-04-06 2010-07-06 E.I. Du Pont De Nemours And Company Clay-binding peptides and methods of use
WO2009018438A1 (en) * 2007-07-31 2009-02-05 Cornell Research Foundation, Inc. Protein discovery using intracellular ribosome display
US9879252B2 (en) 2007-06-13 2018-01-30 Cornell Research Foundation, Inc. Protein discovery using intracellular ribosome display
US7678883B2 (en) * 2007-07-25 2010-03-16 E.I. Du Pont De Nemours And Company Solubility tags for the expression and purification of bioactive peptides
US7951559B2 (en) * 2007-07-25 2011-05-31 E.I. Du Pont De Nemours And Company Recombinant peptide production using a cross-linkable solubility tag
US7829311B2 (en) 2007-07-25 2010-11-09 E.I. Du Pont De Nemours And Company Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate
US7794963B2 (en) 2007-11-02 2010-09-14 E.I. Du Pont De Nemours And Company Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP3629022A1 (de) 2008-07-25 2020-04-01 Richard W. Wagner Proteinscreeningverfahren
JP2012503982A (ja) 2008-09-26 2012-02-16 ワイス・エルエルシー 適合性ディスプレイベクター系
CA2737752A1 (en) * 2008-09-26 2010-04-01 Wyeth Llc Compatible display vector systems
ES2434850T3 (es) * 2008-09-30 2013-12-17 Abbvie Inc. Método mejorado de presentación de ARN
US20100158846A1 (en) * 2008-12-18 2010-06-24 E. I. Du Pont De Nemours And Company Hair-binding peptides
US20100158837A1 (en) 2008-12-18 2010-06-24 E. I. Du Pont De Nemours And Company Iron oxide-binding peptides
US20100158822A1 (en) * 2008-12-18 2010-06-24 E .I. Du Pont De Nemours And Company Peptides that bind to silica-coated particles
US8287845B2 (en) * 2008-12-18 2012-10-16 E I Du Pont De Nemours And Company Hair-binding peptides
KR20120073161A (ko) * 2009-03-30 2012-07-04 존슨 앤드 존슨 컨수머 캄파니즈, 인코포레이티드 화장품 제제 운반용 펩티드계 시스템
WO2010114638A1 (en) 2009-03-30 2010-10-07 E. I. Du Pont De Nemours And Company Peptide-based tooth whitening reagents
EP2546342A4 (de) 2010-03-11 2013-12-04 Riken Verfahren zur auswahl einer polypeptidsequenz, metalloxid oder silicium mit einem bindungspeptid und verwendung davon
MX2013007067A (es) 2010-12-20 2013-11-01 Medimmune Ltd Anticuerpos anti-il-18 y sus usos.
DE102010056289A1 (de) 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
JP5948673B2 (ja) 2011-03-10 2016-07-06 ジーンフロンティア株式会社 リポ多糖混入量を低減した蛋白質合成用組成物、該組成物を用いた蛋白質製造方法
JP6057297B2 (ja) 2011-05-23 2017-01-11 国立研究開発法人理化学研究所 核酸構築物、核酸−蛋白質複合体、及びその利用
CN105164156B (zh) 2012-10-15 2019-04-02 米迪缪尼有限公司 针对β淀粉样蛋白的抗体
WO2014176439A1 (en) 2013-04-25 2014-10-30 Sutro Biopharma, Inc. The use of lambda-gam protein in ribosomal display technology
DK2989239T3 (en) * 2013-04-25 2018-09-03 Sutro Biopharma Inc Selection of Fab fragments using ribosome display technology
US10011830B2 (en) * 2013-05-19 2018-07-03 The Board of Trustee of theLeland Stanford junior University Devices and methods for display of encoded peptides, polypeptides, and proteins on DNA
EP3049558B1 (de) 2013-09-27 2019-07-10 The Board of Trustees of the University of Illionis Verfahren und kit zur erzeugung hochaffiner bindemittel
US10421958B2 (en) 2014-02-05 2019-09-24 Molecular Templates, Inc. Methods of screening, selecting, and identifying cytotoxic recombinant polypeptides based on an interim diminution of ribotoxicity
AU2016263198C1 (en) 2015-05-15 2023-10-05 The General Hospital Corporation Antagonistic anti-tumor necrosis factor receptor superfamily antibodies
US20170145394A1 (en) 2015-11-23 2017-05-25 The Regents Of The University Of California Tracking and manipulating cellular rna via nuclear delivery of crispr/cas9
CN112513250A (zh) * 2018-04-20 2021-03-16 加利福尼亚大学董事会 用于跟踪和操纵细胞rna的融合蛋白和融合核糖核酸
US11174479B2 (en) 2018-08-13 2021-11-16 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for display of encoded peptides, PolyPeptides, and proteins on DNA
CA3153959A1 (en) 2018-09-18 2020-03-26 Merrimack Pharmaceuticals, Inc. Anti-tnfr2 antibodies and uses thereof
CA3131953A1 (en) 2019-03-01 2020-09-10 Merrimack Pharmaceuticals, Inc. Anti-tnfr2 antibodies and uses thereof
WO2022167396A1 (en) 2021-02-02 2022-08-11 Biocopy Gmbh Method for identifying emerging pathogen mutations enabling host cell entry and immune evasion

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2118066T3 (es) * 1989-10-05 1998-09-16 Optein Inc Sintesis y aislamiento, exentos de celulas, de nuevos genes y polipeptidos.
US5723287A (en) * 1992-09-22 1998-03-03 Medical Research Council Recombinant viruses displaying a nonviral polypeptide on their external surface
US5443969A (en) * 1992-10-29 1995-08-22 Rutgers University RNA packaging system
WO1995011922A1 (en) * 1993-10-29 1995-05-04 Affymax Technologies N.V. In vitro peptide and antibody display libraries
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
CA2289691C (en) * 1997-04-23 2008-01-15 Andreas Pluckthun Methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules
CA2291721C (en) * 1997-05-28 2011-08-09 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
ATE311443T1 (de) * 1997-09-03 2005-12-15 Biovation Ltd Methoden zur durchforstung (screenen) von proteinen
US6562622B1 (en) * 1998-05-08 2003-05-13 Diatech Pty, Ltd Continuous in vitro evolution

Also Published As

Publication number Publication date
ATE311444T1 (de) 2005-12-15
EP1268766A2 (de) 2003-01-02
ES2253358T3 (es) 2006-06-01
EP1268766B1 (de) 2005-11-30
AU3944401A (en) 2001-10-15
GB2365429B (en) 2002-07-24
JP2003529369A (ja) 2003-10-07
DK1268766T3 (da) 2006-04-10
WO2001075097A2 (en) 2001-10-11
GB2365429A (en) 2002-02-20
WO2001075097A3 (en) 2002-01-03
US20060177862A1 (en) 2006-08-10
GB0107543D0 (en) 2001-05-16
CA2402452A1 (en) 2001-10-11
US20020076692A1 (en) 2002-06-20
US7074557B2 (en) 2006-07-11
AU2001239444B2 (en) 2004-10-07
DE60115405T2 (de) 2006-08-03

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