DE60102797D1 - Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit - Google Patents

Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit

Info

Publication number
DE60102797D1
DE60102797D1 DE60102797T DE60102797T DE60102797D1 DE 60102797 D1 DE60102797 D1 DE 60102797D1 DE 60102797 T DE60102797 T DE 60102797T DE 60102797 T DE60102797 T DE 60102797T DE 60102797 D1 DE60102797 D1 DE 60102797D1
Authority
DE
Germany
Prior art keywords
particles
distributions
variety
fluorescence intensity
diffusion time
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60102797T
Other languages
English (en)
Other versions
DE60102797T2 (de
Inventor
Peet Kask
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olympus Corp
Original Assignee
Evotec OAI AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec OAI AG filed Critical Evotec OAI AG
Publication of DE60102797D1 publication Critical patent/DE60102797D1/de
Application granted granted Critical
Publication of DE60102797T2 publication Critical patent/DE60102797T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/457—Correlation spectrometry, e.g. of the intensity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J2003/2866—Markers; Calibrating of scan
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE60102797T 2000-02-10 2001-02-09 Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit Expired - Lifetime DE60102797T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US18156400P 2000-02-10 2000-02-10
US181564P 2000-02-10
EP00126774 2000-12-06
EP00126774 2000-12-06
PCT/EP2001/001431 WO2001059416A1 (en) 2000-02-10 2001-02-09 Fluorescence intensity multiple distributions analysis: concurrent determination of diffusion times and molecular brightness

Publications (2)

Publication Number Publication Date
DE60102797D1 true DE60102797D1 (de) 2004-05-19
DE60102797T2 DE60102797T2 (de) 2005-09-08

Family

ID=26071648

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60102797T Expired - Lifetime DE60102797T2 (de) 2000-02-10 2001-02-09 Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit

Country Status (5)

Country Link
EP (1) EP1254353B1 (de)
AT (1) ATE264499T1 (de)
AU (1) AU3172301A (de)
DE (1) DE60102797T2 (de)
WO (1) WO2001059416A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1670943B1 (de) 2003-09-30 2013-11-13 Evotec International GmbH Diagnostische und therapeutische verwendung einer sulfotransferase für alzheimer-krankheit
EP1528379A1 (de) * 2003-10-27 2005-05-04 Evotec Technologies GmbH Verfahren zum Auflösen von Spezies von fluoreszierenden Molekülen in einer Probe
US20080301821A1 (en) 2004-08-11 2008-12-04 Evotec Neurosciences Gmbh Diagnostic and Therapeutic use of a Plasma Membrane Atpase
US20090172827A1 (en) 2005-05-27 2009-07-02 Johannes Pohlner Kcnn3 as diagnostic and therapeutic target for neurodegenerative diseases
US8114620B2 (en) 2005-05-30 2012-02-14 Takeda Pharmaceutical Company Limited Diagnostic and therapeutic target PRKX proteins for neurodegenerative diseases
WO2006128902A1 (en) 2005-06-01 2006-12-07 Evotec Neurosciences Gmbh Diagnostic and therapeutic target slc39a12 proteins for neurodegenerative diseases
DE602006019582D1 (de) 2005-06-16 2011-02-24 Evotec Neurosciences Gmbh Adarb2 proteine als diagnostische und therapeutische targets für neurodegenerative erkrankungen
EP2053397A1 (de) 2007-10-25 2009-04-29 EVOTEC Neurosciences GmbH PPM1E-Proteine und Nukleinsäuren als Targets für neurodegenerative Erkrankungen
DE102013213362A1 (de) * 2013-07-08 2015-01-08 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur Identifizierung und Quantifizierung von emittierenden Teilchen in Systemen

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1595422A (en) * 1977-04-28 1981-08-12 Nat Res Dev Scaning microscopes
EP0884583A1 (de) * 1997-06-10 1998-12-16 Evotec BioSystems GmbH Verfahren zur Charakterisierung von Proben in einem mindestens zweidimensionalen Raum spezifischer physikalischer Eigenschaften
EP1063502A1 (de) * 1999-06-23 2000-12-27 Evotec BioSystems AG Verfahren zur Charakterisierung fluoreszierender Moleküle oder anderer Teilchen unter Verwendung von erzeugenden Funktionen

Also Published As

Publication number Publication date
WO2001059416A1 (en) 2001-08-16
EP1254353B1 (de) 2004-04-14
AU3172301A (en) 2001-08-20
DE60102797T2 (de) 2005-09-08
ATE264499T1 (de) 2004-04-15
EP1254353A1 (de) 2002-11-06

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Legal Events

Date Code Title Description
8332 No legal effect for de
8370 Indication related to discontinuation of the patent is to be deleted
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: OLYMPUS CORPORATION, TOKIO/TOKYO, JP

8328 Change in the person/name/address of the agent

Representative=s name: PATENTANWAELTE VON KREISLER, SELTING, WERNER ET COL

8328 Change in the person/name/address of the agent

Representative=s name: PATENTANWAELTE SCHAEFER EMMEL HAUSFELD, 22043 HAMB