DE60031965D1 - Hochsensitiver nachweis von biomolekülen mittels "phage display" - Google Patents
Hochsensitiver nachweis von biomolekülen mittels "phage display"Info
- Publication number
- DE60031965D1 DE60031965D1 DE60031965T DE60031965T DE60031965D1 DE 60031965 D1 DE60031965 D1 DE 60031965D1 DE 60031965 T DE60031965 T DE 60031965T DE 60031965 T DE60031965 T DE 60031965T DE 60031965 D1 DE60031965 D1 DE 60031965D1
- Authority
- DE
- Germany
- Prior art keywords
- phage
- biomolecules
- target biomolecule
- phage display
- highly sensitive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000002823 phage display Methods 0.000 title 1
- 238000011896 sensitive detection Methods 0.000 title 1
- 102000014914 Carrier Proteins Human genes 0.000 abstract 2
- 108091008324 binding proteins Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12921599P | 1999-04-14 | 1999-04-14 | |
| PCT/US2000/010146 WO2000061790A1 (en) | 1999-04-14 | 2000-04-13 | High sensitivity phage display biomolecule detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60031965D1 true DE60031965D1 (de) | 2007-01-04 |
Family
ID=22438929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60031965T Expired - Lifetime DE60031965D1 (de) | 1999-04-14 | 2000-04-13 | Hochsensitiver nachweis von biomolekülen mittels "phage display" |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6740492B2 (https=) |
| EP (1) | EP1169473B1 (https=) |
| JP (1) | JP2002541818A (https=) |
| AT (1) | ATE346162T1 (https=) |
| AU (1) | AU778776B2 (https=) |
| CA (1) | CA2368875A1 (https=) |
| DE (1) | DE60031965D1 (https=) |
| WO (1) | WO2000061790A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2360282A (en) * | 2000-03-17 | 2001-09-19 | Bioinvent Int Ab | Making and using micro-arrays of biological materials |
| WO2003010198A1 (en) * | 2001-07-26 | 2003-02-06 | Kenton Srl | Identification of specific tumour antigens by selection of cdna libraries with sera and use of said antigens in diagnostic techniques |
| WO2003011902A1 (en) * | 2001-07-27 | 2003-02-13 | Kenton S.R.L. | Identification of specific tumour antigens by selection of cdna libraries with sera |
| MXPA05002934A (es) * | 2002-09-18 | 2006-02-24 | Univ Pennsylvania | Composiciones, metodos y kits para deteccion de un antigeno en una celula y en una mezcla biologica. |
| GB0304832D0 (en) * | 2003-03-04 | 2003-04-09 | Secr Defence | Assay method |
| US8173002B2 (en) * | 2005-02-24 | 2012-05-08 | Life Technologies Corporation | Electro-blotting devices, systems, and kits, and methods for their use |
| EP1885853A2 (en) | 2005-05-27 | 2008-02-13 | Guild Associates, Inc. | Biological detection system and method |
| US8066866B2 (en) * | 2005-10-17 | 2011-11-29 | Lifescan, Inc. | Methods for measuring physiological fluids |
| EP1963494B1 (en) * | 2005-11-29 | 2014-10-08 | The Trustees of The University of Pennsylvania | Phage particle diagnostic reagents |
| US20100044229A1 (en) * | 2008-07-11 | 2010-02-25 | Life Technologies Corporation | Electrophoretically Enhanced Detection of Analytes on a Solid Support |
| USD738527S1 (en) | 2013-05-28 | 2015-09-08 | Life Technologies Corporation | Electroblotting apparatus |
| TWI570243B (zh) | 2015-11-03 | 2017-02-11 | 國立清華大學 | 噬菌體之篩選裝置及方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5223409A (en) * | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| GB9104411D0 (en) * | 1991-03-01 | 1991-04-17 | Elchrom Limited | Improvement of gel composition in gels for submerged gel electrophoresis |
| BE1006312A3 (fr) * | 1991-11-29 | 1994-07-19 | Univ Catholique Louvain | Procede de selection de microorganismes recombinants comportant a leur surface au moins une molecule a activite enzymatique. |
| US5650267A (en) * | 1993-04-27 | 1997-07-22 | Symbiotech, Inc. | Method of detecting compounds utilizing genetically modified lambdoid bacteriophage |
| US5679510A (en) * | 1993-04-27 | 1997-10-21 | Hybridon, Inc. | Quantitative detection of specific nucleic acid sequences using lambdoid bacteriophages linked by oligonucleotides to solid support |
| US6027877A (en) * | 1993-11-04 | 2000-02-22 | Gene Check, Inc. | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification |
| US5702892A (en) * | 1995-05-09 | 1997-12-30 | The United States Of America As Represented By The Department Of Health And Human Services | Phage-display of immunoglobulin heavy chain libraries |
| IL114149A0 (en) * | 1995-06-14 | 1995-10-31 | Yeda Res & Dev | Modified avidin and streptavidin molecules and use thereof |
| WO1997022972A2 (en) * | 1995-12-15 | 1997-06-26 | Pharmacia & Upjohn Company | Method for the simultaneous detection of a therapeutic target for a disease state and its neutralizing antibody-like molecule |
| EP0934526B1 (en) * | 1996-10-08 | 2003-01-08 | U-BISys B.V. | Methods and means for selecting peptides and proteins having specific affinity for a target |
-
2000
- 2000-04-13 EP EP00926011A patent/EP1169473B1/en not_active Expired - Lifetime
- 2000-04-13 AU AU44612/00A patent/AU778776B2/en not_active Ceased
- 2000-04-13 WO PCT/US2000/010146 patent/WO2000061790A1/en not_active Ceased
- 2000-04-13 DE DE60031965T patent/DE60031965D1/de not_active Expired - Lifetime
- 2000-04-13 CA CA002368875A patent/CA2368875A1/en not_active Abandoned
- 2000-04-13 JP JP2000611713A patent/JP2002541818A/ja not_active Withdrawn
- 2000-04-13 AT AT00926011T patent/ATE346162T1/de not_active IP Right Cessation
-
2001
- 2001-10-10 US US09/976,667 patent/US6740492B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002541818A (ja) | 2002-12-10 |
| EP1169473B1 (en) | 2006-11-22 |
| AU778776B2 (en) | 2004-12-23 |
| CA2368875A1 (en) | 2000-10-19 |
| ATE346162T1 (de) | 2006-12-15 |
| AU4461200A (en) | 2000-11-14 |
| EP1169473A1 (en) | 2002-01-09 |
| WO2000061790A1 (en) | 2000-10-19 |
| US6740492B2 (en) | 2004-05-25 |
| US20020110806A1 (en) | 2002-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE60031965D1 (de) | Hochsensitiver nachweis von biomolekülen mittels "phage display" | |
| DE60023936D1 (de) | Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen | |
| ATE421584T1 (de) | Cpg rezeptor (cpg-r) und darauf bezogene methoden | |
| ATE322558T1 (de) | Sensible und multiplexe diagnostische tests zur proteinanalyse | |
| BR0011849A (pt) | Anticorpos enxertados lm609 melhorados | |
| Sannino et al. | Quantitative Assessment of Affinity Selection Performance by Using DNA‐Encoded Chemical Libraries | |
| PT749325E (pt) | Mpleculas biespecificas possuindo utilidades clinicas | |
| DE69233735D1 (de) | Bindungsdomänen des Delta-proteins | |
| ATE336578T1 (de) | Nukleinsäurebindungsproteine | |
| ATE255906T1 (de) | Antikörper gegen cd40 | |
| DE69835143D1 (de) | Selektion von proteinen mittels rns-protein fusionen | |
| EP1601766A4 (en) | METHOD FOR THE FORMATION OF SPECIALLY FRAGMENTED GENBÄNBEN AND BIOLOGICAL REGULATORS THEREFORE BEING SOLVED | |
| WO2000078971A3 (en) | Atp binding cassette transporter protein abc1 popypeptides | |
| ATE446511T1 (de) | Neue fusionsproteine und molekulare bindungsassays | |
| DE69534629D1 (de) | Derivate von zinkfingerproteinen und methoden | |
| DE69831083D1 (de) | Reprimiertes trans-aktivatorsystem zur charakterisierung von protein-protein interaktionen | |
| WO2004099366A3 (en) | Context sensitive parallel optimization of zinc finger dna binding domains | |
| WO2003002724A3 (en) | Proteins, druggable regions of proteins and target analysis for chemistry of therapeutics | |
| ATE389018T1 (de) | Cx3c chemokin-gene von säugetieren | |
| TR200003624T2 (tr) | Yeni poli(ADP-RİBOZ) polimeraz-genleri | |
| DK1180138T3 (da) | Polynukleotidmotor, motorsystem, fremstilling og anvendelse deraf | |
| ATE521623T1 (de) | Neues, bei prostatakrebs exprimiertes gen und verwendungsverfahren | |
| NO20021067L (no) | Apoptin-assosierende protein | |
| WO2001042461A3 (en) | Apoptin-associating protein | |
| SE0201392D0 (sv) | Fusion polypeptide, use thereof and methods employing it |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8332 | No legal effect for de |