DE60023501D1 - Einstufiger allel-austausch in mykobakterium mittels konditionell transduzierendem phagen - Google Patents

Einstufiger allel-austausch in mykobakterium mittels konditionell transduzierendem phagen

Info

Publication number
DE60023501D1
DE60023501D1 DE60023501T DE60023501T DE60023501D1 DE 60023501 D1 DE60023501 D1 DE 60023501D1 DE 60023501 T DE60023501 T DE 60023501T DE 60023501 T DE60023501 T DE 60023501T DE 60023501 D1 DE60023501 D1 DE 60023501D1
Authority
DE
Germany
Prior art keywords
slow
growing mycobacteria
growing
transducing
mycobacteriophage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60023501T
Other languages
English (en)
Other versions
DE60023501T2 (de
Inventor
R Jacobs
Stoyan S Bardarov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Albert Einstein College of Medicine
Original Assignee
Albert Einstein College of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Albert Einstein College of Medicine filed Critical Albert Einstein College of Medicine
Application granted granted Critical
Publication of DE60023501D1 publication Critical patent/DE60023501D1/de
Publication of DE60023501T2 publication Critical patent/DE60023501T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DE60023501T 1999-07-08 2000-07-06 Einstufiger allel-austausch in mykobakterium mittels konditionell transduzierendem phagen Expired - Lifetime DE60023501T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/350,048 US6271034B1 (en) 1999-07-08 1999-07-08 One step allelic exchange in mycobacteria using in vitro generated conditional transducing phages
US350048 1999-07-08
PCT/US2000/040311 WO2001004267A1 (en) 1999-07-08 2000-07-06 One-step allelic exchange in mycobacteria with conditional transducing phage

Publications (2)

Publication Number Publication Date
DE60023501D1 true DE60023501D1 (de) 2005-12-01
DE60023501T2 DE60023501T2 (de) 2006-07-13

Family

ID=23375015

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60023501T Expired - Lifetime DE60023501T2 (de) 1999-07-08 2000-07-06 Einstufiger allel-austausch in mykobakterium mittels konditionell transduzierendem phagen

Country Status (6)

Country Link
US (1) US6271034B1 (de)
EP (1) EP1194526B1 (de)
AT (1) ATE307880T1 (de)
AU (1) AU6803700A (de)
DE (1) DE60023501T2 (de)
WO (1) WO2001004267A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6562348B2 (en) * 1999-07-08 2003-05-13 Albert Einstein College Of Medicine Of Yeshiva University Recombinant M. tuberculosis auxotrophic for leucine and vaccines using same
AU2003209345A1 (en) * 2002-02-19 2003-09-09 Albert Einstein College Of Medicine Of Yeshiva University Attenuated mycobacterium tuberculosis vaccines
US7758874B2 (en) 2002-02-19 2010-07-20 Albert Einstein College Of Medicine Of Yeshiva University Attenuated Mycobacterium tuberculosis vaccines
WO2004066928A2 (en) * 2003-01-24 2004-08-12 Albert Einstein College Of Medicine Of Yeshiva University Use of mycobacterial vaccines in cd4+ or cd8+ lymphocyte-deficient mammals
WO2006078164A2 (en) 2005-01-18 2006-07-27 Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patientenzorg Mycobacteria with mannose cap-deficient lipoarabinomannan
WO2007084353A2 (en) * 2006-01-12 2007-07-26 Albert Einstein College Of Medicine Of Yeshiva University Mycobacterial seca2 mutants
CN101680041A (zh) * 2007-04-18 2010-03-24 康奈尔大学 微生物检测方法和仪器
MX2010000427A (es) 2007-07-12 2010-08-18 Univ Washington State Mutagenesis de intercambio alelico en map.
US9481903B2 (en) 2013-03-13 2016-11-01 Roche Molecular Systems, Inc. Systems and methods for detection of cells using engineered transduction particles
BR112015023108A8 (pt) 2013-03-13 2018-01-23 Geneweave Biosciences Inc sistema de acondicionamento de célula bacteriana, método para acondicionamento de uma molécula de ácido nucléico repórter, composição, método para detectar uma presença ou uma ausência de uma célula bacteriana em uma amostra, vetor, método para detectar um transcrito alvo em uma célula, método para detectar a presença de uma célula bacteriana em uma amostra e kit.
US9540675B2 (en) 2013-10-29 2017-01-10 GeneWeave Biosciences, Inc. Reagent cartridge and methods for detection of cells
US10351452B2 (en) 2014-01-29 2019-07-16 Synphagen Llc Compositions for in vivo expression of therapeutic sequences in the microbiome
US10351893B2 (en) 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261568B1 (en) * 1997-06-11 2001-07-17 Institut Pasteur Attenuated recombinant mycobacteria useful as immunogens or as vaccine components
US5972700A (en) * 1997-09-26 1999-10-26 Albert Einstein College Of Medicine Of Yeshiva University TM4 conditional shuttle phasmids and uses thereof

Also Published As

Publication number Publication date
EP1194526B1 (de) 2005-10-26
ATE307880T1 (de) 2005-11-15
EP1194526A4 (de) 2003-07-30
US6271034B1 (en) 2001-08-07
WO2001004267A9 (en) 2002-08-01
AU6803700A (en) 2001-01-30
EP1194526A1 (de) 2002-04-10
DE60023501T2 (de) 2006-07-13
WO2001004267A1 (en) 2001-01-18

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