TW200504211A - New nitrile hydratase - Google Patents

New nitrile hydratase

Info

Publication number
TW200504211A
TW200504211A TW093116724A TW93116724A TW200504211A TW 200504211 A TW200504211 A TW 200504211A TW 093116724 A TW093116724 A TW 093116724A TW 93116724 A TW93116724 A TW 93116724A TW 200504211 A TW200504211 A TW 200504211A
Authority
TW
Taiwan
Prior art keywords
microbe
nitrile
compounds
concentration
nitrile hydratase
Prior art date
Application number
TW093116724A
Other languages
Chinese (zh)
Other versions
TWI321151B (en
Inventor
Kaoru Furuya
Akira Tamaki
Shinichiro Nagasawa
Ayano Suzuki
Original Assignee
Asahi Chemical Ind
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Ind filed Critical Asahi Chemical Ind
Publication of TW200504211A publication Critical patent/TW200504211A/en
Application granted granted Critical
Publication of TWI321151B publication Critical patent/TWI321151B/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01084—Nitrile hydratase (4.2.1.84)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A process for producing an amide compound with the use of a nitrile hydratase of high thermal stability capable of maintaining high activity even in the presence of high-concentration nitrile compound as a substrate and amide compound as a product. A recombinant microbe was produced by searching for a novel microbe capable of expression of the desired nitrile hydratase in nature, obtaining relevant gene from cells of the microbe or treatment product thereof, performing cloning of the gene and introducing the same in a different type of microbe for expression. With the use thereof, nitrile compounds can be efficiently converted to corresponding amide compounds even by the reaction conducted at high temperature and in the presence of high-concentration nitrile compounds and high-concentration amide compounds.
TW093116724A 2003-06-10 2004-06-10 New nitrile hydratase TW200504211A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2003164773 2003-06-10

Publications (2)

Publication Number Publication Date
TW200504211A true TW200504211A (en) 2005-02-01
TWI321151B TWI321151B (en) 2010-03-01

Family

ID=33508825

Family Applications (1)

Application Number Title Priority Date Filing Date
TW093116724A TW200504211A (en) 2003-06-10 2004-06-10 New nitrile hydratase

Country Status (6)

Country Link
JP (1) JP4108095B2 (en)
KR (1) KR100806991B1 (en)
CN (1) CN1806047A (en)
AU (1) AU2004245849B2 (en)
TW (1) TW200504211A (en)
WO (1) WO2004108942A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200634151A (en) * 2004-12-09 2006-10-01 Asahi Chemical Ind Transformant expressing nitrile hydratase
JP2007181404A (en) * 2005-12-29 2007-07-19 Institute Of Physical & Chemical Research Isonitrile hydrolase and method for producing amine
WO2008004473A1 (en) * 2006-07-06 2008-01-10 University Of Tsukuba Novel protein complex, method for maturation of cobalt-type low-molecular-weight nitrile hydratase using the protein complex, matured cobalt-type low-molecular-weight nitrile hydratase, and method using the nitrile hydratase
JP5080787B2 (en) * 2006-11-15 2012-11-21 達彦 小林 Protein modification method, nitrile hydratase maturation method, mature nitrile hydratase production method, amide compound production method using mature nitrile hydratase
KR101995103B1 (en) * 2014-06-06 2019-07-03 미쯔비시 케미컬 주식회사 Improved nitrile hydratase
US10655152B2 (en) 2014-09-30 2020-05-19 Solenis Technologies, L.P. Method for producing an amide
CN111334495B (en) * 2020-03-12 2022-04-29 宜昌东阳光生化制药有限公司 Method for preparing dexamidoketoprofen
CN113444714B (en) * 2021-07-29 2022-06-21 浙江大学杭州国际科创中心 Beta subunit mutant nitrile hydratase mutant and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153415A (en) * 1998-04-29 2000-11-28 Board Of Trustees Operating Michigan State University Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus

Also Published As

Publication number Publication date
KR100806991B1 (en) 2008-02-25
WO2004108942A1 (en) 2004-12-16
AU2004245849B2 (en) 2008-10-09
TWI321151B (en) 2010-03-01
JPWO2004108942A1 (en) 2006-07-20
KR20060016115A (en) 2006-02-21
CN1806047A (en) 2006-07-19
AU2004245849A1 (en) 2004-12-16
JP4108095B2 (en) 2008-06-25

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Legal Events

Date Code Title Description
MM4A Annulment or lapse of patent due to non-payment of fees