DE60022541T2 - Polynukleotid- amplifikationsverfahren - Google Patents

Polynukleotid- amplifikationsverfahren Download PDF

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Publication number
DE60022541T2
DE60022541T2 DE60022541T DE60022541T DE60022541T2 DE 60022541 T2 DE60022541 T2 DE 60022541T2 DE 60022541 T DE60022541 T DE 60022541T DE 60022541 T DE60022541 T DE 60022541T DE 60022541 T2 DE60022541 T2 DE 60022541T2
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DE
Germany
Prior art keywords
amount
amplification
analyte
polynucleotide
amplicon produced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60022541T
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German (de)
English (en)
Other versions
DE60022541D1 (de
Inventor
Kiyotada Nunomura
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Gen Probe Inc
Original Assignee
Gen Probe Inc
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Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of DE60022541D1 publication Critical patent/DE60022541D1/de
Publication of DE60022541T2 publication Critical patent/DE60022541T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE60022541T 1999-07-23 2000-07-21 Polynukleotid- amplifikationsverfahren Expired - Lifetime DE60022541T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14543299P 1999-07-23 1999-07-23
PCT/US2000/020034 WO2001007661A2 (en) 1999-07-23 2000-07-21 Polynucleotide amplification method

Publications (2)

Publication Number Publication Date
DE60022541D1 DE60022541D1 (de) 2005-10-13
DE60022541T2 true DE60022541T2 (de) 2006-06-14

Family

ID=22513089

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60022541T Expired - Lifetime DE60022541T2 (de) 1999-07-23 2000-07-21 Polynukleotid- amplifikationsverfahren

Country Status (10)

Country Link
US (2) US6294338B1 (enExample)
EP (1) EP1238100B1 (enExample)
JP (1) JP4796252B2 (enExample)
AT (1) ATE304061T1 (enExample)
AU (1) AU781570B2 (enExample)
CA (1) CA2378408C (enExample)
DE (1) DE60022541T2 (enExample)
DK (1) DK1238100T3 (enExample)
ES (1) ES2248096T3 (enExample)
WO (1) WO2001007661A2 (enExample)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE421999T1 (de) * 1999-07-09 2009-02-15 Gen Probe Inc Nachweis von hiv-1 durch amplifizierung von nukleinsäuren
US6783934B1 (en) 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
US6582920B2 (en) 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
WO2003002753A2 (en) * 2001-06-28 2003-01-09 Chiron Corporation Diagnostic assays for parvovirus b19
US6852491B2 (en) * 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
WO2003091426A1 (en) 2001-10-12 2003-11-06 Spectral Genomics, Inc. Compilations of nucleic acids and arrays and methods of using them
US7439346B2 (en) 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
US20030175709A1 (en) * 2001-12-20 2003-09-18 Murphy George L. Method and system for depleting rRNA populations
AU2003229666A1 (en) * 2002-05-08 2003-11-11 Pharmacia Italia Spa A chimeric reverse transcriptase and methods for identifying telomerase inhibitors
EP2292801B1 (en) 2002-06-14 2018-08-08 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
KR100890885B1 (ko) 2003-03-31 2009-03-31 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
AU2004276722B2 (en) 2003-05-19 2009-03-12 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample
EP1678329A4 (en) * 2003-10-30 2008-07-02 Tufts New England Medical Ct PRENATAL DIAGNOSIS USING CELL-FREE FEDERAL DNA IN FRUIT WATER
EP3167961A1 (en) * 2004-04-08 2017-05-17 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20080176209A1 (en) * 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20060099567A1 (en) * 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
EP2975139B1 (en) 2004-09-30 2019-11-06 Gen-Probe Incorporated Assay for detecting and quantifying hiv-1
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
US20060190192A1 (en) * 2005-01-21 2006-08-24 Medical College Of Ohio Databases for assessing nucleic acids
KR100654342B1 (ko) * 2005-02-07 2006-12-08 삼성전자주식회사 이미지 센서
WO2007050990A2 (en) * 2005-10-27 2007-05-03 Rosetta Inpharmatics Llc Nucleic acid amplification using non-random primers
EP2206779B1 (en) 2005-12-06 2013-02-13 Life Technologies Corporation Reverse transcription primers and methods of design
WO2009137369A1 (en) * 2008-05-03 2009-11-12 Tufts Medical Center, Inc. Neonatal salivary genomics
EP2456887B1 (en) 2009-07-21 2015-11-25 Gen-Probe Incorporated Methods and compositions for quantitative detection of nucleic acid sequences over an extended dynamic range
CA2896129C (en) 2010-01-22 2015-12-29 Gen-Probe Incorporated Probes for detecting the presence of trichomonas vaginalis in a sample
WO2011103274A1 (en) 2010-02-17 2011-08-25 Gen-Probe Incorporated Compositions and methods to detect atopobium vaginae nucleic acid
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
CA2806670A1 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
EP3272886B1 (en) 2011-04-25 2019-09-25 Gen-Probe Incorporated Compositions and methods for detecting bv-associated bacterial nucleic acid
EP2753713B1 (en) 2011-09-08 2017-07-19 Gen-Probe Incorporated Compositions and methods for detecting bv-associated bacterial nucleic acid
HK1217117A1 (zh) 2012-12-20 2016-12-23 生物马特里卡公司 用於使pcr试剂稳定化的制剂和方法
EP3942931A1 (en) 2014-06-10 2022-01-26 Biomatrica, INC. Stabilization of thrombocytes at ambient temperatures
EP4242628B1 (en) 2015-12-08 2025-07-02 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5283174A (en) 1987-09-21 1994-02-01 Gen-Probe, Incorporated Homogenous protection assay
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
EP0731174B1 (en) * 1989-07-11 2004-11-17 Gen-Probe Incorporated Nucleic acid sequence amplification methods
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
GB9116042D0 (en) 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
ATE257860T1 (de) 1991-08-02 2004-01-15 Biomerieux Bv Quantifikation von nukleinsäuren
CA2135073C (en) 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
CA2141430C (en) 1992-08-04 2009-05-12 Sherrol H. Mcdonough Nucleic acid sequence amplification
US5457027A (en) 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
JP3594598B2 (ja) * 1993-07-09 2004-12-02 アクゾ・ノベル・エヌ・ベー 改良された核酸定量方法
KR100189229B1 (ko) 1993-07-23 1999-06-01 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 핵산 증폭을 강화하는 방법
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
WO1996040990A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5962665A (en) * 1997-06-16 1999-10-05 Abbott Laboratories Nucleic acid primers and probes for detecting HIV-1 and HIV-2
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification

Also Published As

Publication number Publication date
AU781570B2 (en) 2005-06-02
US6294338B1 (en) 2001-09-25
ATE304061T1 (de) 2005-09-15
AU6232200A (en) 2001-02-13
CA2378408C (en) 2010-07-06
DK1238100T3 (da) 2006-01-23
WO2001007661A3 (en) 2002-07-11
DE60022541D1 (de) 2005-10-13
US6908735B2 (en) 2005-06-21
CA2378408A1 (en) 2001-02-01
EP1238100A2 (en) 2002-09-11
JP4796252B2 (ja) 2011-10-19
US20020106668A1 (en) 2002-08-08
ES2248096T3 (es) 2006-03-16
JP2003510020A (ja) 2003-03-18
EP1238100B1 (en) 2005-09-07
WO2001007661A2 (en) 2001-02-01

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