DE60018758D1 - Chemilumineszenz-substrate von hydrolytischen enzymen wie phosphatasen - Google Patents
Chemilumineszenz-substrate von hydrolytischen enzymen wie phosphatasenInfo
- Publication number
- DE60018758D1 DE60018758D1 DE60018758T DE60018758T DE60018758D1 DE 60018758 D1 DE60018758 D1 DE 60018758D1 DE 60018758 T DE60018758 T DE 60018758T DE 60018758 T DE60018758 T DE 60018758T DE 60018758 D1 DE60018758 D1 DE 60018758D1
- Authority
- DE
- Germany
- Prior art keywords
- lumi
- hydrolytic
- enzyms
- phosphatases
- attached
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003301 hydrolyzing effect Effects 0.000 title abstract 4
- 239000000758 substrate Substances 0.000 title abstract 2
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 title 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical group N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- 108091005804 Peptidases Proteins 0.000 abstract 2
- -1 acridinium esters Chemical class 0.000 abstract 2
- KXDHJXZQYSOELW-UHFFFAOYSA-N Carbamic acid Chemical class NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 abstract 1
- 108090000371 Esterases Proteins 0.000 abstract 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 abstract 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 abstract 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 102000005262 Sulfatase Human genes 0.000 abstract 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N acridine Chemical class C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 abstract 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-O acridine;hydron Chemical class C1=CC=CC2=CC3=CC=CC=C3[NH+]=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-O 0.000 abstract 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical group [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 108010022683 guanidinobenzoate esterase Proteins 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 235000019833 protease Nutrition 0.000 abstract 1
- 235000019419 proteases Nutrition 0.000 abstract 1
- 108060007951 sulfatase Proteins 0.000 abstract 1
- 239000011593 sulfur Chemical group 0.000 abstract 1
- 229910052717 sulfur Chemical group 0.000 abstract 1
- 150000007970 thio esters Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
- C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
- C07D491/10—Spiro-condensed systems
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
- C07D219/04—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the ring system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
- C07F9/576—Six-membered rings
- C07F9/64—Acridine or hydrogenated acridine ring systems
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14664899P | 1999-07-30 | 1999-07-30 | |
| US146648P | 1999-07-30 | ||
| PCT/US2000/020429 WO2001009372A1 (en) | 1999-07-30 | 2000-07-27 | Chemiluminescent substrates of hydrolytic enzymes such as phosphatases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60018758D1 true DE60018758D1 (de) | 2005-04-21 |
| DE60018758T2 DE60018758T2 (de) | 2006-01-19 |
Family
ID=22518335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60018758T Expired - Lifetime DE60018758T2 (de) | 1999-07-30 | 2000-07-27 | Chemiliumineszierendes Substrat von Hydrolasen wie Phosphatasen |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1203091B1 (de) |
| JP (1) | JP3819294B2 (de) |
| AT (1) | ATE291096T1 (de) |
| AU (1) | AU6381900A (de) |
| DE (1) | DE60018758T2 (de) |
| WO (1) | WO2001009372A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6783948B1 (en) | 1999-07-30 | 2004-08-31 | Bayer Corporation | Chemiluminescent acridinium compounds and analogues thereof as substrates of hydrolytic enzymes |
| CA2448824A1 (en) * | 2001-06-01 | 2002-12-12 | F. Hoffmann-La Roche Ag | Novel compounds for chemiluminescense procedures |
| US6664043B2 (en) * | 2001-07-03 | 2003-12-16 | Bayer Corporation | Acridinium ester labels having hydrophilic modifiers |
| US6723851B2 (en) | 2001-10-31 | 2004-04-20 | Quest Diagnostics Investment Incorporated | Chemiluminescent compounds and use thereof |
| GB0208987D0 (en) * | 2002-04-19 | 2002-05-29 | Amersham Biosciences Uk Ltd | Methods for measuring protein kinase and phosphatase activity |
| AU2003263917A1 (en) * | 2002-08-20 | 2004-03-11 | Quest Diagnostics Investments Incorporated | Hydrophilic chemiluminescent acridinium labeling reagents |
| US7309615B2 (en) * | 2002-09-27 | 2007-12-18 | Siemens Medical Solutions Diagnostic | High quantum yield acridinium compounds and their uses in improving assay sensitivity |
| US7319041B2 (en) * | 2002-09-27 | 2008-01-15 | Siemens Medical Solutions Diagnostic | Applications of acridinium compounds and derivatives in homogeneous assays |
| FR2850378B1 (fr) | 2003-01-29 | 2005-03-04 | Bio Merieux | Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram+ par rapport a des bacteries a gram- |
| CN103868913B (zh) * | 2014-02-11 | 2017-07-14 | 中生北控生物科技股份有限公司 | 碱性磷酸酶的酶促化学发光底物液 |
| CN110003106B (zh) * | 2019-04-24 | 2020-11-06 | 郑州原理生物科技有限公司 | 一种吖啶的制备方法 |
| CN114245830A (zh) * | 2019-08-07 | 2022-03-25 | 深圳华大基因科技有限公司 | 含吖啶酯的化合物及将其用于基于化学发光的单色测序的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4745181A (en) * | 1986-10-06 | 1988-05-17 | Ciba Corning Diagnostics Corp. | Polysubstituted aryl acridinium esters |
| US4810636A (en) * | 1986-12-09 | 1989-03-07 | Miles Inc. | Chromogenic acridinone enzyme substrates |
| US5656426A (en) * | 1988-08-01 | 1997-08-12 | Chiron Diagnostics Corporation | Functionaized hydrophilic acridinium esters |
| JPH07509245A (ja) * | 1992-07-20 | 1995-10-12 | デイド・ベーリング・マルブルク・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | 新規化学発光化合物およびその使用方法 |
| US5772926A (en) * | 1997-05-13 | 1998-06-30 | Lumigen, Inc. | Chemiluminescent reactions using dihydroxyaromatic compounds and heterocyclic enol phosphates |
| DE69940433D1 (de) * | 1998-08-11 | 2009-04-02 | Siemens Healthcare Diagnostics | Im nahen infrarot chemilumineszente akridinium derivate und ihre verwendung |
-
2000
- 2000-07-27 AT AT00950764T patent/ATE291096T1/de not_active IP Right Cessation
- 2000-07-27 AU AU63819/00A patent/AU6381900A/en not_active Abandoned
- 2000-07-27 JP JP2001513627A patent/JP3819294B2/ja not_active Expired - Lifetime
- 2000-07-27 DE DE60018758T patent/DE60018758T2/de not_active Expired - Lifetime
- 2000-07-27 WO PCT/US2000/020429 patent/WO2001009372A1/en active IP Right Grant
- 2000-07-27 EP EP00950764A patent/EP1203091B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001009372A1 (en) | 2001-02-08 |
| JP3819294B2 (ja) | 2006-09-06 |
| ATE291096T1 (de) | 2005-04-15 |
| WO2001009372B1 (en) | 2001-06-14 |
| AU6381900A (en) | 2001-02-19 |
| JP2003528938A (ja) | 2003-09-30 |
| EP1203091B1 (de) | 2005-03-16 |
| EP1203091A1 (de) | 2002-05-08 |
| DE60018758T2 (de) | 2006-01-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC. (N.D.GES.D, US |
|
| 8328 | Change in the person/name/address of the agent |
Representative=s name: MAIER, D., DIPL.-ING. UNIV., PAT.-ASS., 81739 MUEN |