DE508597C - Vessel, especially for breeding bacteria - Google Patents
Vessel, especially for breeding bacteriaInfo
- Publication number
- DE508597C DE508597C DE1930508597D DE508597DD DE508597C DE 508597 C DE508597 C DE 508597C DE 1930508597 D DE1930508597 D DE 1930508597D DE 508597D D DE508597D D DE 508597DD DE 508597 C DE508597 C DE 508597C
- Authority
- DE
- Germany
- Prior art keywords
- vessel
- bead
- asbestos
- wall
- thickening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
Gefäß, insbesondere zum Züchten von Bakterien Um Bakterien in Reagenzgläsern oder sonstigen Gefäßen zu züchten, hat man die letzteren bisher in der Regel mit einem Watte-oder Papierstoffpfropfen abgeschlossen. Ein solcher Verschluß ist, wie sich herausgestellt hat, nicht keimdicht und außerdem unsauber, da stets Teilchen des Verschlusses in die Kultur fallen. Ferner kann der obere Rand des Gefäßes bei einem solchen Abschluß nicht ohne weiteres durch Abflammung sterilisiert werden.Vessel, especially for growing bacteria. Around bacteria in test tubes or other vessels to grow, the latter has usually been used up to now finished with a wad of cotton or paper. One such lock is how has turned out to be not germ-proof and also unclean, since there are always particles of the closure fall into the culture. Furthermore, the upper edge of the vessel can be such a closure cannot be sterilized by flame sterilization without further ado.
Die Sterilisation der mit Watte oder Papier gestopften Reagenzgläser war überdies nur unter Schädigung der Stopfen möglich, die bei zweckgemäßer Sterilisation 2oo° C zum Unbrauchbarwerden führte. Wurden aber in sterilen Flaschen, Reagenzgläsern usw. Arzneien und ähnliche Lösungen bereitet, so kamen feinste Stopfenteilchen später in die Spritzen vier Ärzte und verhinderten eine sachgemäße Injektion.The sterilization of test tubes stuffed with cotton wool or paper moreover, was only possible with damage to the stoppers, which was the case with proper sterilization 2oo ° C made it unusable. But were in sterile bottles, test tubes etc. If medicines and similar solutions were prepared, the finest stopper particles came later four doctors in the syringes and prevented an appropriate injection.
Die vorliegende Erfindung bezweckt die Beseitigung dieser Nachteile und kennzeichnet sich durch die Zwischenlegung einer Asbestschicht -zwischen Deckel und Gefäßwandung. Auf diese Weise wird ein sauberer Abschluß erhalten, die Sterilisation bis 200° ermöglicht und außerdem die Sterilisation des Gefäßrandes durch eine Abflammung ermöglicht. Ferner wird auf diese Weise ein völlig keimdichter Abschluß durch den Asbestgewebering, der die zutretende Luft filtriert, erreicht. In der beiliegenden Zeichnung ist der Erfindungsgegenstand in mehreren Ausführungsformen in Abb. i bis 3 im Schnitt, teilweise in Ansicht, dargestellt.The present invention aims to overcome these drawbacks and is characterized by the interposition of a layer of asbestos between the lid and vessel wall. In this way a clean finish is obtained, the sterilization up to 200 ° and also the sterilization of the edge of the vessel by flame treatment enables. Furthermore, in this way a completely germ-tight seal is achieved by the Asbestos fabric ring that filters the incoming air. In the enclosed Drawing is the subject matter of the invention in several embodiments in Fig. I to 3 shown in section, partially in view.
Gemäß Abb. i ist das zur Aufnahme des Nährbodens dienende Reagenzglas a an seiner Oberseite mit einer wulstartigen Verdickung b versehen. Zwischen dem umgebogenen Rand c des Reagenzglases a und dem erwähnten Wulst b ist ein Asbestschnurring d eingesetzt. Der Abschluß des Reagenzglases erfolgt durch einen zweckmäßig aus Glas besteh-enden Deckel e, der mit seinem unigebördelten Rand f über den Asbestschnurring d greift. Auf diese Weise wird ein völlig keimdichter Abschluß des Reagenzglases a erzielt. Da die Asbestschnur d nicht brennbar ist, kann der obere Glasrand durch Abflammung ohne weiteres sterilisiert werden.According to Fig. I, the test tube a used to hold the nutrient medium is provided on its upper side with a bead-like thickening b. An asbestos cord ring d is inserted between the bent edge c of the test tube a and the aforementioned bead b. The test tube is closed by a cover e, which is expediently made of glass and which grips with its unflared edge f over the asbestos cord ring d. In this way, a completely germ-tight seal of the test tube a is achieved. Since the asbestos cord d is not flammable, the upper edge of the glass can be easily sterilized by flaming.
Um zu verhindern, daß sich an der Innenwandung des Reagenzglases irgendwelche Schmutzteile oder Keime absetzen können, verläuft die Innenwandung völlig glatt, auch an der Stelle, an der sich an der Außenseite die wulstartige Verdickung b befindet.To prevent any Dirt or germs can settle, the inner wall runs completely smooth, also at the point where the bead-like thickening b is located on the outside.
Abb.2 zeigt einen entsprechenden keimdichten Abschluß mittels der Asbestschnur d und den Deckel e an einer sogenannten Kulturflasche g, die. ebenfalls mit einer wulstartigen Verdickung b zum Tragen des Asbestringes versehen ist.Fig.2 shows a corresponding germ-tight seal by means of the Asbestos cord d and the lid e on a so-called culture bottle g, the. Likewise is provided with a bead-like thickening b for carrying the asbestos ring.
Abb. 3 zeigt eine weitere Ausbildung des Erfindungsgegenstandes in der Hinsicht, daß an dem Glasdeckel e ein Haken h artgeschmolzen ist, der zum Anbringen des in den -Nährboden L einzuhängenden Gutes k, beispielsweise Katgutfadens, Organteile usw., dient. Auf diese Weise ist es möglich, die Bakterien in einfachster Weise zu züchten. Durch Ablieben des mit dem Haken h versehenen Deckels e ist ein schnelles Wechseln der N ährbodenflüssigkeit möglich. Der keimdichte Abschluß erfolgt auch in diesem Falle durch den Asb.estschnurring d, über welchen der Deckel e mit seinem umgebördelten Rand f greift.Fig. 3 shows a further embodiment of the subject matter of the invention in that a hook h is art-melted on the glass cover e, which hook is used to attach the goods k to be hung in the nutrient base L , for example catgut, organ parts, etc. In this way it is possible to grow the bacteria in the simplest possible way. By leaving the cover e with the hook h off, the nutrient medium liquid can be changed quickly. In this case, too, the germ-tight seal is provided by the Asb.estschnurring d, over which the lid e grips with its flanged edge f.
Gegebenenfalls kann auch beispielsweise bei Reagenzgläsern der Wulst b wegfallen und die Außenwandung in der üblichen Weise glatt verlaufen. Der Asbestschnurring d wird hierbei in der gleichen Weise wie nach Abb. r von dem umgebördelten Rand des Deckels e umfaßt.If necessary, the bead can also be used, for example, in test tubes b are omitted and the outer wall runs smoothly in the usual way. The asbestos cord ring d is here in the same way as in Fig. r from the beaded edge of the cover e includes.
Claims (5)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE508597T | 1930-01-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE508597C true DE508597C (en) | 1930-10-01 |
Family
ID=6547011
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE1930508597D Expired DE508597C (en) | 1930-01-30 | 1930-01-30 | Vessel, especially for breeding bacteria |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE508597C (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITUD20090027A1 (en) * | 2009-02-06 | 2010-08-07 | Alifax Holding S P A | MEDICAL DEVICE FOR VERIFICATION OF THE PRESENCE OF MICRO-ORGANISMS OR BACTERIA IN A ORGAN OR BIOLOGICAL FABRIC SAMPLE, A DIAGNOSTIC EQUIPMENT INCLUDING SUCH A MEDICAL DEVICE AND ITS PROCEDURE |
-
1930
- 1930-01-30 DE DE1930508597D patent/DE508597C/en not_active Expired
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITUD20090027A1 (en) * | 2009-02-06 | 2010-08-07 | Alifax Holding S P A | MEDICAL DEVICE FOR VERIFICATION OF THE PRESENCE OF MICRO-ORGANISMS OR BACTERIA IN A ORGAN OR BIOLOGICAL FABRIC SAMPLE, A DIAGNOSTIC EQUIPMENT INCLUDING SUCH A MEDICAL DEVICE AND ITS PROCEDURE |
WO2010089659A2 (en) | 2009-02-06 | 2010-08-12 | Alifax Holding Spa | Medical device to verify the presence of microorganisms or bacteria in a sample of an organ or a biological tissue, diagnostic apparatus comprising said medical device and relative method |
WO2010089659A3 (en) * | 2009-02-06 | 2011-04-21 | Alifax Holding Spa | Medical device to verify the presence of microorganisms or bacteria in a sample of an organ or a biological tissue, diagnostic apparatus comprising said medical device and relative method |
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