DE454876C - Culture tubes in commercial form - Google Patents

Culture tubes in commercial form

Info

Publication number
DE454876C
DE454876C DEM94474D DEM0094474D DE454876C DE 454876 C DE454876 C DE 454876C DE M94474 D DEM94474 D DE M94474D DE M0094474 D DEM0094474 D DE M0094474D DE 454876 C DE454876 C DE 454876C
Authority
DE
Germany
Prior art keywords
culture
tubes
culture tubes
commercial form
ready
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEM94474D
Other languages
German (de)
Inventor
Dr Theodor Moll
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck KGaA
Original Assignee
E Merck AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E Merck AG filed Critical E Merck AG
Priority to DEM94474D priority Critical patent/DE454876C/en
Application granted granted Critical
Publication of DE454876C publication Critical patent/DE454876C/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube

Landscapes

  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Materials For Medical Uses (AREA)

Description

Kulturröhrchen in handelsfähiger Form. Die in der Bakteriologie gebräuchlichen Kulturröhrchen mit gebrauchsfertigen flüssigen oder halbfesten Nährböden sind zum Versand oder handelsmäßigen Bezug ungeeignet, da es nicht möglich ist, den zum Wachstum der Bakterien erforderlichen Watteverschluß der Röhrchen vor der Berührung mit den Nährmedien zu schützen. Eine besondere Art von Kulturgefäßen, die zur Versendung flüssiger Nährmedien und zur Züchtung von Keimen dienen sollen, ist zum erstenmal in der Patentschrift 301 179 beschrieben worden. Zwar sind in diesen Kulturgefäßen die flüssigen Nährböden vor der Verunreinigung und vor dem Vertrocknen sicher, können auch darin verschickt werden und bleiben zum Anlegen von Kulturen geeignet. Für einen handelsmäßigen Bezug von gebrauchsfertigen Nährböden und für allgemeine Anwendung kommen diese Gefäße aber nicht in Frage, da ihre Herstellung zu teuer und ihre Hand= habung zu kompliziert ist. Auch sind diese Kulturgefäße nur für flüssige Nährböden geeignet, und das Anlegen von Kulturen ist nur so weit möglich, als es sich um Impfmaterial handelt, das durch den sehr engen trichterförmigen Hals durchgeführt werden kann.Culture tubes in a marketable form. The culture tubes commonly used in bacteriology with ready-to-use liquid or semi-solid nutrient media are unsuitable for shipping or commercially available, as it is not possible to protect the cotton-wool seal on the tubes, which is necessary for the growth of bacteria, from contact with the culture media. A special type of culture vessel, which is intended to be used for sending liquid nutrient media and for growing germs, has been described for the first time in patent specification 301 179. Although the liquid nutrient media in these culture vessels are safe from contamination and from drying out, they can also be shipped in them and remain suitable for creating cultures. However, these vessels are out of the question for a commercial purchase of ready-to-use nutrient media and for general use, since their production is too expensive and their handling is too complicated. These culture vessels are also only suitable for liquid nutrient media, and the creation of cultures is only possible as long as it is an inoculation material that can be passed through the very narrow, funnel-shaped neck.

Es ist nun gelungen, eine Form von Kulturröhrchen zu finden, die es ermöglicht, sowohl flüssige wie halbfeste Nährböden zur Ausführung von hakteriologischen Untersuchungen handelsmäßig beziehen und vorrätig halten zu können. Es läßt sich mit diesen Kulturröhrchen in der gleichen Weise arbeiten wie mit den in der Bakteriologie allgemein verwendeten Kulturröhrchen.It has now been possible to find a form of culture tube that can enables both liquid and semi-solid culture media for the execution of bacteriological Obtain examinations commercially and keep them in stock. It can be work with these culture tubes in the same way as with those in bacteriology commonly used culture tubes.

Das Prinzip dieser Kulturröhrchen beruht darauf, daßdie flüssigen oder halbfesten Nährbodenmedien in geeignete Glasröhrchen eingeschmolzen und darin sterilisiert werden. Um diese Röhrchen als gebrauchsfertige Kulturröhrchen verwenden zu können, wird nach dem Öffnen eine sterile Kappe aus Glas oder anderem Material, die noch etwas Watte enthalten kann, aufgesetzt. Diese Kappe kann jedem einzelnen Röhrchen beigefügt werden, beispielsweise indem sie auf das geschlossene Röhrchen aufgesetzt und durch einen Klebstreifen oder mit einem anderen Dichtungsmaterial. vor Verunreinigungen geschützt und steril gehalten wird. Es kann aber auch eine Kappe für mehrere Röhrchen benutzt werden, indem die Kappen in sterilem Verpackungsmaterial geliefert oder vor dem Gebrauch durch Ausbrennen oder Ausglühen sterilisiert werden. Das Öffnen der Röhrchen erfolgt durch Absprengen der oberen Kuppe dadurch, daß eine eingeritzte Feilmarke mit einem glühenden Glasstäbchen berührt wird.The principle of these culture tubes is based on the fact that the liquid or semi-solid culture medium in suitable glass tubes and melted in sterilized. To use these tubes as ready-to-use culture tubes after opening a sterile cap made of glass or other material, which may still contain some cotton wool, put on. This cap can be used by any individual Tubes can be attached, for example by placing them on the closed tube put on and by an adhesive tape or with another sealing material. is protected from contamination and kept sterile. But it can also be a Multi-tube cap can be used by placing the caps in sterile packaging material or sterilized by burning out or baking before use. The tubes are opened by breaking off the top tip by making a The incised file mark is touched with a glowing glass stick.

Claims (2)

PATENTANSPRÜCHE: r. Kulturröhrcben in handelsfähiger Form, dadurch- gekennzeichnet, dafi gebrauchsfertige Nährböden in zugeschmolzene Glasröhrchen eingeschmolzen werden. PATENT CLAIMS: r. Culture tubes in a marketable form, characterized in that ready-to-use culture media are melted into sealed glass tubes. 2. Kulturröhrchen nach Anspruch z, dadurch gekennzeichnet, daB man als sterilen Verschluß der geöffneten, im Gebrauch befindlichen Kulturröhrchen eine sterile Kappe aus Glas oder anderem Material verwendet.2. Culture tube according to claim z, characterized in that one is considered sterile Close the opened culture tubes in use with a sterile cap made of glass or other material.
DEM94474D 1926-05-11 1926-05-11 Culture tubes in commercial form Expired DE454876C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEM94474D DE454876C (en) 1926-05-11 1926-05-11 Culture tubes in commercial form

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEM94474D DE454876C (en) 1926-05-11 1926-05-11 Culture tubes in commercial form

Publications (1)

Publication Number Publication Date
DE454876C true DE454876C (en) 1928-01-19

Family

ID=7322713

Family Applications (1)

Application Number Title Priority Date Filing Date
DEM94474D Expired DE454876C (en) 1926-05-11 1926-05-11 Culture tubes in commercial form

Country Status (1)

Country Link
DE (1) DE454876C (en)

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