DE499580C - Process for the preparation of antitoxic pneumococcal serum - Google Patents

Process for the preparation of antitoxic pneumococcal serum

Info

Publication number
DE499580C
DE499580C DEI28347D DEI0028347D DE499580C DE 499580 C DE499580 C DE 499580C DE I28347 D DEI28347 D DE I28347D DE I0028347 D DEI0028347 D DE I0028347D DE 499580 C DE499580 C DE 499580C
Authority
DE
Germany
Prior art keywords
antitoxic
preparation
pneumococcal
serum
toxins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEI28347D
Other languages
German (de)
Inventor
Dr Richard Bieling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IG Farbenindustrie AG
Original Assignee
IG Farbenindustrie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IG Farbenindustrie AG filed Critical IG Farbenindustrie AG
Priority to DEI28347D priority Critical patent/DE499580C/en
Application granted granted Critical
Publication of DE499580C publication Critical patent/DE499580C/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1275—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Streptococcus (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Description

Verfahren zur Darstellung von antitoxischem Pneumokokkenserum Es ist bekannt, daß man durch Züchtung von Streptokokkenstämmen, welche von Scharlachkranken gewonnen sind, Toxine erzeugen kann, wenn man die betreffenden Streptokokkenstämme auf einer Fleischbrühe züchtet, welcher zweckmäßig kleinere Mengen von Blut zugesetzt worden sind und nach einem Wachstum von etwa rund 7 Tagen die Bakterien abtötet oder entfernt. Nach Mitteilung in der Literatur können auch andere nicht von Scharlachkranken stammende Streptokokkenstämme in ähnlicher Weise zur Herstellung von Streptokokkentoxin benutzt werden.Method for the preparation of antitoxic pneumococcal serum It is known that by breeding Streptococcus strains, which from scarlet fever sufferers are obtained, toxins can be generated by using the relevant streptococcal strains grows on a meat broth to which smaller amounts of blood are added appropriately and kill the bacteria after around 7 days of growth or removed. According to information in the literature, others cannot suffer from scarlet fever either strains of streptococcus derived in a similar manner for the production of streptococcal toxin to be used.

Es wurde nun gefunden, daß die Verimpfung von Pneumokokken auf diese Nährböden ebenfalls zur Darstellung von Toxinen führt. Infolgedessen ist man in der Lage durch Behandlung von Pferden mit solchen Pneumokokkentoxinen Antitoxine Hervorzurufen, deren Wirkung darin besteht, daß sie die Behandlungstoxine neutralisieren. Es ist auf diese Weise gelungen, ein wertvolles antitoxisches Serum gegen Infektionen mit toxinbildenden Pneumokokken herzustellen.It has now been found that the inoculation of pneumococci on this Culture media also leads to the depiction of toxins. As a result, you're in able to antitoxins by treating horses with such pneumococcal toxins To evoke the effect of which is to neutralize treatment toxins. In this way it has been possible to create a valuable anti-toxic serum against infections with toxin-producing pneumococci.

Beispiel. 5oo ccm einer Rindi3eisclibrühe werden mit 5 ccm frischem defebriniertem Hammelblut versetzt und mit einer 24stündigen Pneumokokkenkulturbeimpft. Nach einem Wachstum von 6 bis 8 Tagen wird die Kultur mit Toluol überschüttet und mehrfach durchgeschüttelt, bis die sämtlichen Pneumokokken abgetötet sind. An Stelle dieser üblichen Methode kann man auch die bewachsene Fleischbrühe durch ein Bakterienfilter keimfrei filtrieren.Example. 500 cc of beef ice cream broth are mixed with 5 cc of fresh defebrinated mutton blood and inoculated with a 24-hour pneumococcal culture. After growth of 6 to 8 days, the culture is showered with toluene and Shaken several times until all of the pneumococci have been killed. Instead of This usual method can also filter the overgrown meat broth through a bacterial filter filter germ-free.

Die Behandlung der Pferde mit so hergestelltem Toxin erfolgt in der für die Gewinnung von antitoxischen Seren üblichen Weise.The treatment of the horses with toxin produced in this way takes place in the the usual way to obtain antitoxic serums.

Claims (1)

PATENTANSPRUCH: Verfahren zur Herstellung von antitoxischem Pneumokokkenserum, dadurch gekennzeichnet, daß man Pneumokokken auf eiweißhaltigen flüssigen Nährböden züchtet, nach Ablauf der Vermehrungsperiode die Bakterien mechanisch entfernt oder abtötet und die so gewonnenen Toxine Tieren injiziert, worauf das Antitoxin in der üblichen Weise gewonnen wird.PATENT CLAIM: Process for the production of antitoxic pneumococcal serum, characterized in that one pneumococci on protein-containing liquid nutrient media cultivates, removes the bacteria mechanically after the end of the multiplication period or kills and injects the toxins thus obtained animals, whereupon the antitoxin in the usual way is obtained.
DEI28347D 1926-06-17 1926-06-17 Process for the preparation of antitoxic pneumococcal serum Expired DE499580C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEI28347D DE499580C (en) 1926-06-17 1926-06-17 Process for the preparation of antitoxic pneumococcal serum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEI28347D DE499580C (en) 1926-06-17 1926-06-17 Process for the preparation of antitoxic pneumococcal serum

Publications (1)

Publication Number Publication Date
DE499580C true DE499580C (en) 1930-06-10

Family

ID=7187003

Family Applications (1)

Application Number Title Priority Date Filing Date
DEI28347D Expired DE499580C (en) 1926-06-17 1926-06-17 Process for the preparation of antitoxic pneumococcal serum

Country Status (1)

Country Link
DE (1) DE499580C (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1003917B (en) * 1955-12-23 1957-03-07 Dr Miguel Liberio Gratacos Process for the preparation of a complex concentrate produced by bacterial decomposition for the treatment of allergic diseases in general, asthma and rheumatism

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1003917B (en) * 1955-12-23 1957-03-07 Dr Miguel Liberio Gratacos Process for the preparation of a complex concentrate produced by bacterial decomposition for the treatment of allergic diseases in general, asthma and rheumatism

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