DE4290124T1 - Verfahren und Zusammensetzungen zur Expression von biologisch aktiven, eukaryontischen Cytochromen P450 in Bakterien - Google Patents

Verfahren und Zusammensetzungen zur Expression von biologisch aktiven, eukaryontischen Cytochromen P450 in Bakterien

Info

Publication number
DE4290124T1
DE4290124T1 DE4290124T DE4290124T DE4290124T1 DE 4290124 T1 DE4290124 T1 DE 4290124T1 DE 4290124 T DE4290124 T DE 4290124T DE 4290124 T DE4290124 T DE 4290124T DE 4290124 T1 DE4290124 T1 DE 4290124T1
Authority
DE
Germany
Prior art keywords
bacteria
expression
compositions
methods
biologically active
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE4290124T
Other languages
English (en)
Other versions
DE4290124B4 (de
Inventor
Charles W Fisher
Henry J Barnes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Texas System
Original Assignee
University of Texas System
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Texas System filed Critical University of Texas System
Publication of DE4290124T1 publication Critical patent/DE4290124T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C12N9/0085—Steroid 17 alpha-monooxygenase (1.14.99.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C12Y114/99009—Steroid 17-alpha-monooxygenase (1.14.99.9), i.e. cytochrome-P450-steroid-17-alpha-hydroxylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE4290124T 1991-01-10 1992-01-10 Verfahren und Zusammensetzungen zur Expression von biologisch aktiven, eukaryontischen Cytochromen P450 in Bakterien Pending DE4290124T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/640,473 US5240831A (en) 1991-01-10 1991-01-10 Methods and compositions for the expression of biologically active eukaryotic cytochrome p45os in bacteria
PCT/US1992/000168 WO1992013076A1 (en) 1991-01-10 1992-01-10 Methods and compositions for the expression of biologically active eukaryotic cytochrome p450s in bacteria

Publications (1)

Publication Number Publication Date
DE4290124T1 true DE4290124T1 (de) 1994-04-28

Family

ID=24568401

Family Applications (2)

Application Number Title Priority Date Filing Date
DE4290124A Expired - Lifetime DE4290124B4 (de) 1991-01-10 1992-01-10 Verfahren und Zusammensetzungen zur Expression von biologisch aktiven, eukaryontischen Cytochromen P450 in Bakterien
DE4290124T Pending DE4290124T1 (de) 1991-01-10 1992-01-10 Verfahren und Zusammensetzungen zur Expression von biologisch aktiven, eukaryontischen Cytochromen P450 in Bakterien

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE4290124A Expired - Lifetime DE4290124B4 (de) 1991-01-10 1992-01-10 Verfahren und Zusammensetzungen zur Expression von biologisch aktiven, eukaryontischen Cytochromen P450 in Bakterien

Country Status (6)

Country Link
US (1) US5240831A (de)
AU (1) AU1226092A (de)
CA (1) CA2100245C (de)
DE (2) DE4290124B4 (de)
GB (1) GB2266719B (de)
WO (1) WO1992013076A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5460961A (en) * 1988-06-14 1995-10-24 La Region Wallonne Human myeloperoxidase and its therapeutic application
US5846799A (en) * 1988-06-14 1998-12-08 La Region Wallone Human myeloperoxidase and its therapeutic application
US5420027A (en) * 1991-01-10 1995-05-30 Board Of Regents, The University Of Texas System Methods and compositions for the expression of biologically active fusion proteins comprising a eukaryotic cytochrome P450 fused to a reductase in bacteria
US5547868A (en) * 1993-06-09 1996-08-20 Regents Of The University Of California Cholesterol disposal fusion enzymes
US5516674A (en) * 1993-08-31 1996-05-14 North Carolina State University Insecticide resistance associated cytochrome 450
US5886157A (en) * 1994-02-10 1999-03-23 Vanderbilt University Expression and purification of human cytochrome P450
JP2938352B2 (ja) * 1994-09-30 1999-08-23 オリエンタル酵母工業株式会社 組換え人マトリライシンの製造方法
JP3315827B2 (ja) * 1994-10-07 2002-08-19 オリエンタル酵母工業株式会社 活性型ヒトａｌｔの製造方法
US5919682A (en) * 1995-08-24 1999-07-06 Board Of Regents, University Of Texas System Overproduction of neuronal nitric oxide synthase
US9255256B2 (en) * 1996-07-17 2016-02-09 Btg International Limited Expression of functional cytochorome P450 monooxygenase system in enterobacteria
GB9615032D0 (en) * 1996-07-17 1996-09-04 Univ Dundee Enzyme system
AU4149597A (en) * 1996-08-30 1998-03-19 Somatogen, Inc. Production of heme and recombinant hemoproteins
EP2088188B1 (de) 1997-04-16 2011-08-31 Unigene Laboratories, Inc. Direkte Expression von Peptiden in Nährmedien
JP3176342B2 (ja) * 1997-08-13 2001-06-18 ファイザー製薬株式会社 薬剤代謝の評価方法及び前記方法用の試薬組成物
WO1999008812A1 (en) * 1997-08-20 1999-02-25 The University Of Rochester Functional bacterial/mammalian cytochrome p450 chimera
US7157418B1 (en) 1998-07-22 2007-01-02 Osprey Pharmaceuticals, Ltd. Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders
US20030215421A1 (en) * 1999-07-21 2003-11-20 Mcdonald John R. Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders
US7642233B2 (en) * 2004-09-15 2010-01-05 William Marsh Rice University Enhancing recombinant hemoglobin production by co-expression with alpha hemoglobin stabilizing protein
US20070166792A1 (en) * 2004-09-15 2007-07-19 Olson John S Increasing hemoglobin and other heme protein production in bacteria by co-expression of heme transport genes
EP1799234A4 (de) * 2004-09-15 2009-02-18 William Marsh Rice Univeristy Verbesserung der rekombinanten hämoglobin-produktion durch koexpression mit alpha-hämoglobin stabilisierendem protein
CA2589937C (en) * 2004-12-07 2014-04-22 Lonza Ag Rhamnose promoter expression system
US10261086B2 (en) 2013-01-10 2019-04-16 Amrita Vishwa Vidyapeetham Differential cerebrospinal fluid reactivity to PFDN5-alpha for detection of B-cell acute lymphoblastic leukemia
CN112359027B (zh) * 2021-01-13 2021-04-13 凯莱英生命科学技术(天津)有限公司 细胞色素p450酶突变体及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766068A (en) * 1984-06-16 1988-08-23 Agency Of Industrial Science And Technology Cytochrome P-450MC gene, expression plasmid carrying the said gene, yeasts transformed with the said plasmid and a process for producing cytochrome P-450MC by culturing the said transformant yeasts
JPS615783A (ja) * 1984-06-16 1986-01-11 Agency Of Ind Science & Technol ラット肝チトクロムＰ―４５０ｃ遺伝子の大腸菌内発現を目的とした発現用プラスミドｐＭＧ１の構築
US5045471A (en) * 1986-12-02 1991-09-03 The Regents Of The University Of California Cloned DNA for P450scc and expression thereof
IL90207A (en) * 1988-05-06 1994-07-31 Roussel Uclaf Biochemical oxidation of steroids and genetically engineered cells to be used therefor
CN1042567A (zh) * 1988-09-23 1990-05-30 吉斯特-布罗卡迪斯公司 类固醇多步氧化方法和所用的遗传工程细胞
US5212296A (en) * 1989-09-11 1993-05-18 E. I. Du Pont De Nemours And Company Expression of herbicide metabolizing cytochromes

Also Published As

Publication number Publication date
DE4290124B4 (de) 2008-11-20
WO1992013076A1 (en) 1992-08-06
US5240831A (en) 1993-08-31
AU1226092A (en) 1992-08-27
GB2266719A (en) 1993-11-10
GB2266719B (en) 1995-02-15
CA2100245A1 (en) 1992-07-11
CA2100245C (en) 2003-07-08
GB9314201D0 (en) 1993-09-01

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