DE4021458C1 - - Google Patents
Info
- Publication number
- DE4021458C1 DE4021458C1 DE4021458A DE4021458A DE4021458C1 DE 4021458 C1 DE4021458 C1 DE 4021458C1 DE 4021458 A DE4021458 A DE 4021458A DE 4021458 A DE4021458 A DE 4021458A DE 4021458 C1 DE4021458 C1 DE 4021458C1
- Authority
- DE
- Germany
- Prior art keywords
- vector
- exon
- cloning
- host cells
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Diabetes (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4021458A DE4021458C1 (US06566495-20030520-M00010.png) | 1990-07-05 | 1990-07-05 | |
US07/725,608 US5252475A (en) | 1990-07-05 | 1991-07-03 | Methods and vectors for selectively cloning exons |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4021458A DE4021458C1 (US06566495-20030520-M00010.png) | 1990-07-05 | 1990-07-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE4021458C1 true DE4021458C1 (US06566495-20030520-M00010.png) | 1991-08-29 |
Family
ID=6409725
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE4021458A Expired - Lifetime DE4021458C1 (US06566495-20030520-M00010.png) | 1990-07-05 | 1990-07-05 |
Country Status (2)
Country | Link |
---|---|
US (1) | US5252475A (US06566495-20030520-M00010.png) |
DE (1) | DE4021458C1 (US06566495-20030520-M00010.png) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0656954A1 (en) * | 1993-01-05 | 1995-06-14 | JARVIK, Jonathan Wallace | Method for producing tagged genes, transcripts, and proteins |
WO1997002346A2 (en) * | 1995-06-30 | 1997-01-23 | Genzyme Corporation | Novel human chromosome 16 genes, compositions, methods of making and using same |
WO1997048797A1 (en) * | 1996-06-17 | 1997-12-24 | Genzyme Corporation | Novel human chromosome 16 genes, compositions, methods of making and using same |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4965199A (en) * | 1984-04-20 | 1990-10-23 | Genentech, Inc. | Preparation of functional human factor VIII in mammalian cells using methotrexate based selection |
EP0742285A1 (en) * | 1995-05-10 | 1996-11-13 | Rijksuniversiteit Leiden | Method of isolating exonic gene segments of eukaryotic genes, exon trapping vectors for use therein |
US6030806A (en) * | 1995-06-30 | 2000-02-29 | Landes; Gregory M. | Human chromosome 16 genes, compositions, methods of making and using same |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
-
1990
- 1990-07-05 DE DE4021458A patent/DE4021458C1/de not_active Expired - Lifetime
-
1991
- 1991-07-03 US US07/725,608 patent/US5252475A/en not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
---|
Cell 46, S. 973-982, 1986 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0656954A1 (en) * | 1993-01-05 | 1995-06-14 | JARVIK, Jonathan Wallace | Method for producing tagged genes, transcripts, and proteins |
EP0656954A4 (en) * | 1993-01-05 | 1997-04-09 | Jonathan Wallace Jarvik | PRODUCTION METHOD OF MARKED GENES; TRANSCRIPTS AND PROTEINS. |
WO1997002346A2 (en) * | 1995-06-30 | 1997-01-23 | Genzyme Corporation | Novel human chromosome 16 genes, compositions, methods of making and using same |
WO1997002346A3 (en) * | 1995-06-30 | 1997-04-17 | Genzyme Corp | Novel human chromosome 16 genes, compositions, methods of making and using same |
WO1997048797A1 (en) * | 1996-06-17 | 1997-12-24 | Genzyme Corporation | Novel human chromosome 16 genes, compositions, methods of making and using same |
Also Published As
Publication number | Publication date |
---|---|
US5252475A (en) | 1993-10-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8100 | Publication of the examined application without publication of unexamined application | ||
D1 | Grant (no unexamined application published) patent law 81 | ||
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |