DE4021458C1 - - Google Patents

Info

Publication number
DE4021458C1
DE4021458C1 DE4021458A DE4021458A DE4021458C1 DE 4021458 C1 DE4021458 C1 DE 4021458C1 DE 4021458 A DE4021458 A DE 4021458A DE 4021458 A DE4021458 A DE 4021458A DE 4021458 C1 DE4021458 C1 DE 4021458C1
Authority
DE
Germany
Prior art keywords
vector
exon
cloning
host cells
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE4021458A
Other languages
German (de)
English (en)
Inventor
Michael Dr. 7800 Freiburg De Reth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority to DE4021458A priority Critical patent/DE4021458C1/de
Priority to US07/725,608 priority patent/US5252475A/en
Application granted granted Critical
Publication of DE4021458C1 publication Critical patent/DE4021458C1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE4021458A 1990-07-05 1990-07-05 Expired - Lifetime DE4021458C1 (US06566495-20030520-M00010.png)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE4021458A DE4021458C1 (US06566495-20030520-M00010.png) 1990-07-05 1990-07-05
US07/725,608 US5252475A (en) 1990-07-05 1991-07-03 Methods and vectors for selectively cloning exons

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4021458A DE4021458C1 (US06566495-20030520-M00010.png) 1990-07-05 1990-07-05

Publications (1)

Publication Number Publication Date
DE4021458C1 true DE4021458C1 (US06566495-20030520-M00010.png) 1991-08-29

Family

ID=6409725

Family Applications (1)

Application Number Title Priority Date Filing Date
DE4021458A Expired - Lifetime DE4021458C1 (US06566495-20030520-M00010.png) 1990-07-05 1990-07-05

Country Status (2)

Country Link
US (1) US5252475A (US06566495-20030520-M00010.png)
DE (1) DE4021458C1 (US06566495-20030520-M00010.png)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0656954A1 (en) * 1993-01-05 1995-06-14 JARVIK, Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
WO1997002346A2 (en) * 1995-06-30 1997-01-23 Genzyme Corporation Novel human chromosome 16 genes, compositions, methods of making and using same
WO1997048797A1 (en) * 1996-06-17 1997-12-24 Genzyme Corporation Novel human chromosome 16 genes, compositions, methods of making and using same

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965199A (en) * 1984-04-20 1990-10-23 Genentech, Inc. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
EP0742285A1 (en) * 1995-05-10 1996-11-13 Rijksuniversiteit Leiden Method of isolating exonic gene segments of eukaryotic genes, exon trapping vectors for use therein
US6030806A (en) * 1995-06-30 2000-02-29 Landes; Gregory M. Human chromosome 16 genes, compositions, methods of making and using same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Cell 46, S. 973-982, 1986 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0656954A1 (en) * 1993-01-05 1995-06-14 JARVIK, Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
EP0656954A4 (en) * 1993-01-05 1997-04-09 Jonathan Wallace Jarvik PRODUCTION METHOD OF MARKED GENES; TRANSCRIPTS AND PROTEINS.
WO1997002346A2 (en) * 1995-06-30 1997-01-23 Genzyme Corporation Novel human chromosome 16 genes, compositions, methods of making and using same
WO1997002346A3 (en) * 1995-06-30 1997-04-17 Genzyme Corp Novel human chromosome 16 genes, compositions, methods of making and using same
WO1997048797A1 (en) * 1996-06-17 1997-12-24 Genzyme Corporation Novel human chromosome 16 genes, compositions, methods of making and using same

Also Published As

Publication number Publication date
US5252475A (en) 1993-10-12

Similar Documents

Publication Publication Date Title
DE69431285T2 (de) Herstellungsmethode von markierten genen; transcripten und proteinen
DE69034135T2 (de) DNS-Konstrukten zur Aktivierung und Veränderung der Expression von endogenen Genen
DE69604775T2 (de) Genexpressionuntersuchung durch aufzeigen von cdna 3'-restriktionsfragmenten
DE69621507T2 (de) Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen
DE3752284T2 (de) Hormon-rezeptor-verbindungen und methoden
DE3884610T2 (de) Durch sterole regulierbare promoterelemente.
DE69024440T2 (de) Verfahren zum spezifischen ersetzen einer genkopie, die sich in einem rezeptorengenom befindet, durch genintegration
DE69011101T2 (de) Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.
DE69233106T2 (de) Verfahren zur Herstellung von Einzelsträndigen "stem-loop" DNS
DE3889021T2 (de) Verfahren zum Transformieren von Zellen.
DE69323131T2 (de) Rekombinierte alkalische phosphatase aus kälberdarm
DE3834636A1 (de) Verfahren zur analyse von laengenpolymorphismen in dna-bereichen
DE69706307T2 (de) Mais promoter-sequenz zur bevorzugten genexpression im blatt und stengel
EP0776363B1 (de) Ribozym-bibliothek, ihre herstellung und ihre verwendung
DE69029113T2 (de) Verfahren zur Bestimmung von Allelen für zystische Fibrose
DE4021458C1 (US06566495-20030520-M00010.png)
DE69424209T2 (de) Tumorsuppressor-Gen Merlin und dessen Verwendungen
DE69936867T2 (de) Dns sequenz, verfahren für dessen nachweis und herstellung und benutzung
DE69224327T2 (de) Nucleotidsequenzen
DE69937444T2 (de) Isolierte und aufgereinigte nukleinsäuren, welche ein gen enthalten, welches in hopfendrüsen exprimiert wird
EP1588172A2 (de) Verfahren zur identifizierung bhs-spezifischer proteine und fragmente davon
DE60011187T2 (de) Ligation von doppelsträngigen DNAs in Gegenwart von RecA
DE69122246T2 (de) Identifikation von neuen medikamenten und reagenzien
DE60116500T2 (de) Dna/rna-einbettungsmedium und verfahren zur verwendung
DE60013410T2 (de) Verfahren zur analyse der genexpressions-frequenz

Legal Events

Date Code Title Description
8100 Publication of the examined application without publication of unexamined application
D1 Grant (no unexamined application published) patent law 81
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee