DE3889918D1 - Nachweis einzelner moleküle. - Google Patents

Nachweis einzelner moleküle.

Info

Publication number
DE3889918D1
DE3889918D1 DE3889918T DE3889918T DE3889918D1 DE 3889918 D1 DE3889918 D1 DE 3889918D1 DE 3889918 T DE3889918 T DE 3889918T DE 3889918 T DE3889918 T DE 3889918T DE 3889918 D1 DE3889918 D1 DE 3889918D1
Authority
DE
Germany
Prior art keywords
single molecules
detecting single
detecting
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3889918T
Other languages
English (en)
Other versions
DE3889918T2 (de
Inventor
Edgar Shera
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Energy
Original Assignee
US Department of Energy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Energy filed Critical US Department of Energy
Application granted granted Critical
Publication of DE3889918D1 publication Critical patent/DE3889918D1/de
Publication of DE3889918T2 publication Critical patent/DE3889918T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
    • G01N15/0211—Investigating a scatter or diffraction pattern
    • G01N2015/0222—Investigating a scatter or diffraction pattern from dynamic light scattering, e.g. photon correlation spectroscopy
    • G01N2015/1027—
DE3889918T 1987-10-07 1988-09-16 Nachweis einzelner moleküle. Expired - Fee Related DE3889918T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/105,376 US4793705A (en) 1987-10-07 1987-10-07 Single molecule tracking
PCT/US1988/003193 WO1989003525A1 (en) 1987-10-07 1988-09-16 Single molecule tracking

Publications (2)

Publication Number Publication Date
DE3889918D1 true DE3889918D1 (de) 1994-07-07
DE3889918T2 DE3889918T2 (de) 1995-01-19

Family

ID=22305471

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3889918T Expired - Fee Related DE3889918T2 (de) 1987-10-07 1988-09-16 Nachweis einzelner moleküle.

Country Status (6)

Country Link
US (1) US4793705A (de)
EP (1) EP0381694B1 (de)
JP (1) JPH03500449A (de)
CA (1) CA1326556C (de)
DE (1) DE3889918T2 (de)
WO (1) WO1989003525A1 (de)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US4979824A (en) * 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US5209834A (en) * 1992-03-09 1993-05-11 The United States Of America As Represented By The United States Department Of Energy Ordered transport and identification of particles
US5422712A (en) * 1992-04-01 1995-06-06 Toa Medical Electronics Co., Ltd. Apparatus for measuring fluorescent spectra of particles in a flow
CA2155186A1 (en) * 1993-02-01 1994-08-18 Kevin M. Ulmer Methods and apparatus for dna sequencing
US5827663A (en) * 1995-02-03 1998-10-27 The Regents Of The University Of California Method and apparatus for reducing solvent luminescence background emissions
GB2316081B (en) * 1996-08-12 2001-05-09 Bio Dot Ltd Dispensing of particles
US6221654B1 (en) * 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
EP1017987B1 (de) 1997-01-31 2005-06-15 The Horticulture And Food Research Institute Of New Zealand Limited Optische vorrichtung und methode
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
JP3935509B2 (ja) 1997-02-12 2007-06-27 ワイ． チャン，ユージーン ポリマー分析のための方法および製品
US6049380A (en) * 1997-11-12 2000-04-11 Regents Of The University Of California Single molecule identification using selected fluorescence characteristics
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
SE9800360D0 (sv) 1998-02-06 1998-02-06 Goeteborg University Science I Method, apparatus and flow cell for high sensitivity detection of fluorescent molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
AT410718B (de) * 1998-10-28 2003-07-25 Schindler Hansgeorg Dr Vorrichtung zur visualisierung von molekülen
DE69930310T3 (de) 1998-12-14 2009-12-17 Pacific Biosciences of California, Inc. (n. d. Ges. d. Staates Delaware), Menlo Park Kit und methode zur nukleinsäuresequenzierung einzelner moleküle durch polymerase synthese
WO2000070080A1 (en) * 1999-05-17 2000-11-23 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
CA2412567A1 (en) 2000-06-07 2001-12-13 Li-Cor, Inc. Charge-switch nucleotides
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
AU2002237689B2 (en) 2000-11-29 2008-01-10 Xy, Llc. System to separate frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations
EP1354064A2 (de) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US20020102596A1 (en) * 2001-01-31 2002-08-01 Davis Lloyd Mervyn Methods for detecting interaction of molecules with surface-bound reagents
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
AU2002258997A1 (en) * 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
US7118907B2 (en) * 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
EP1436424A4 (de) * 2001-09-18 2005-11-16 Us Genomics Inc Differentielles tagging von polymeren zur hochaufgelösten linearen analyse
AU2003238796A1 (en) * 2002-05-28 2003-12-12 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US7282330B2 (en) * 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
EP1545203B1 (de) 2002-08-01 2016-10-19 Xy, Llc Mit niederdruck arbeitendes spermazellentrennsystem
BRPI0313476B1 (pt) 2002-08-15 2015-06-23 Xy Llc Citômetro de fluxo de alta resolução
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
US20040166514A1 (en) * 2002-11-19 2004-08-26 Singulex, Inc. Detection of target molecules through interaction with probes
EP2306174B1 (de) 2003-03-28 2016-05-11 Inguran, LLC Durchflusszytometriedüse zur Orientierung von Partikeln und entsprechendes Verfahren
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
WO2004091795A2 (en) * 2003-04-10 2004-10-28 U.S. Genomics, Inc. Manipulation of polymers in a microchannel
NZ544103A (en) 2003-05-15 2010-10-29 Xy Llc Efficient haploid cell sorting for flow cytometer systems
WO2005019419A2 (en) * 2003-07-31 2005-03-03 Singulex, Inc. Co-detection of single polypeptide and polynucleotide molecules
US20080021674A1 (en) * 2003-09-30 2008-01-24 Robert Puskas Methods for Enhancing the Analysis of Particle Detection
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005067692A2 (en) 2004-01-13 2005-07-28 U.S. Genomics, Inc. Detection and quantification of analytes in solution using polymers
EP1716254B1 (de) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Verfahren zur analyse von polynukleotidsequenzen
US20050221408A1 (en) * 2004-03-19 2005-10-06 U.S. Genomics, Inc. Compositions and methods for detection of single molecules
WO2005095590A2 (en) 2004-03-29 2005-10-13 Monsanto Technology Llc Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
JP2008512084A (ja) 2004-05-25 2008-04-24 ヘリコス バイオサイエンシーズ コーポレイション 核酸の配列決定のための方法およびデバイス
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7833147B2 (en) 2004-07-22 2010-11-16 Inguran, LLC. Process for enriching a population of sperm cells
US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
EP1805500A4 (de) * 2004-09-28 2008-05-07 Singulex Inc System und verfahren zur spektroskopischen analyse einzelner partikel
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20070128083A1 (en) * 2005-07-18 2007-06-07 U.S. Genomics, Inc. Microfluidic methods and apparatuses for sample preparation and analysis
US20070042406A1 (en) * 2005-07-18 2007-02-22 U.S. Genomics, Inc. Diffusion mediated clean-up of a target carrier fluid
EP1904653A4 (de) * 2005-07-18 2010-04-14 Us Genomics Inc Mikrofluidische verfahren und vorrichtungen zur probenvorbereitung und -analyse
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US7871777B2 (en) * 2005-12-12 2011-01-18 The United States Of America As Represented By The Department Of Health And Human Services Probe for nucleic acid sequencing and methods of use
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
EP3156799B1 (de) 2006-04-04 2024-01-24 Novilux, LLC Analysator und verfahren zur hochempfindlichen detektion von analyten
JP5308328B2 (ja) 2006-04-04 2013-10-09 シングレックス，インコーポレイテッド トロポニンの分析のための高感度のシステムおよび方法
US8821799B2 (en) * 2007-01-26 2014-09-02 Palo Alto Research Center Incorporated Method and system implementing spatially modulated excitation or emission for particle characterization with enhanced sensitivity
US9164037B2 (en) 2007-01-26 2015-10-20 Palo Alto Research Center Incorporated Method and system for evaluation of signals received from spatially modulated excitation and emission to accurately determine particle positions and distances
CN103543094B (zh) 2007-12-19 2017-06-09 神谷来克斯公司 单分子检测用扫描分析器和使用方法
US8629981B2 (en) * 2008-02-01 2014-01-14 Palo Alto Research Center Incorporated Analyzers with time variation based on color-coded spatial modulation
US8373860B2 (en) 2008-02-01 2013-02-12 Palo Alto Research Center Incorporated Transmitting/reflecting emanating light with time variation
US20090234202A1 (en) * 2008-03-05 2009-09-17 Goix Philippe J Method and compositions for highly sensitive detection of molecules
GB2464183A (en) * 2008-09-19 2010-04-14 Singulex Inc Sandwich assay
AU2010259022B2 (en) * 2009-06-08 2016-05-12 Singulex, Inc. Highly sensitive biomarker panels
JP2013525820A (ja) 2010-05-06 2013-06-20 シングレクス、インコーポレイテッド 関節リウマチの発症に関するリスクを診断し、病期分類し、予測し、治療レスポンダーを同定するための方法
US9029800B2 (en) 2011-08-09 2015-05-12 Palo Alto Research Center Incorporated Compact analyzer with spatial modulation and multiple intensity modulated excitation sources
US8723140B2 (en) 2011-08-09 2014-05-13 Palo Alto Research Center Incorporated Particle analyzer with spatial modulation and long lifetime bioprobes
WO2014062719A2 (en) 2012-10-15 2014-04-24 Nanocellect Biomedical, Inc. Systems, apparatus, and methods for sorting particles
CN103091294B (zh) * 2013-01-21 2015-08-05 中南民族大学 应用于骨髓干细胞运动轨迹追踪的细胞标记检测方法
CN107561053B (zh) * 2016-07-01 2020-04-28 清华大学 一种单分子检测方法
CN108195801B (zh) * 2017-11-17 2020-11-24 北京林业大学 单分子水平观测气孔保卫细胞膜蛋白分布和动态的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61186854A (ja) * 1985-02-14 1986-08-20 Fuji Photo Film Co Ltd 超純水中のバクテリア数測定装置

Also Published As

Publication number Publication date
EP0381694A1 (de) 1990-08-16
CA1326556C (en) 1994-01-25
JPH03500449A (ja) 1991-01-31
US4793705A (en) 1988-12-27
EP0381694A4 (en) 1991-08-28
EP0381694B1 (de) 1994-06-01
DE3889918T2 (de) 1995-01-19
WO1989003525A1 (en) 1989-04-20

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Legal Events

Date Code Title Description
8380 Miscellaneous part iii

Free format text: DER INHABER IST ZU AENDERN IN: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, OAKLAND, CALIF., US

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee