DE3805150A1 - Gentechnologisches verfahren zur herstellung von polypeptiden - Google Patents
Gentechnologisches verfahren zur herstellung von polypeptidenInfo
- Publication number
- DE3805150A1 DE3805150A1 DE3805150A DE3805150A DE3805150A1 DE 3805150 A1 DE3805150 A1 DE 3805150A1 DE 3805150 A DE3805150 A DE 3805150A DE 3805150 A DE3805150 A DE 3805150A DE 3805150 A1 DE3805150 A1 DE 3805150A1
- Authority
- DE
- Germany
- Prior art keywords
- galactosidase
- gene
- fragment
- polypeptide
- codons
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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- 229960000723 ampicillin Drugs 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 229960003773 calcitonin (salmon synthetic) Drugs 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000004026 insulin derivative Substances 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 229920001308 poly(aminoacid) Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 108010068072 salmon calcitonin Proteins 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Diabetes (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Priority Applications (14)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3805150A DE3805150A1 (de) | 1987-04-11 | 1988-02-19 | Gentechnologisches verfahren zur herstellung von polypeptiden |
| DE8888105438T DE3877035D1 (de) | 1987-04-11 | 1988-04-06 | Gentechnologisches verfahren zur herstellung von polypeptiden. |
| EP88105438A EP0286956B1 (de) | 1987-04-11 | 1988-04-06 | Gentechnologisches Verfahren zur Herstellung von Polypeptiden |
| AT88105438T ATE84076T1 (de) | 1987-04-11 | 1988-04-06 | Gentechnologisches verfahren zur herstellung von polypeptiden. |
| FI881617A FI881617A7 (fi) | 1987-04-11 | 1988-04-07 | Genteknologiskt foerfarande foer framstaellning av polypeptider. |
| NZ224184A NZ224184A (en) | 1987-04-11 | 1988-04-08 | Modification of beta-galactosidase regulatory gene; use in genetic engineering |
| NO881548A NO881548L (no) | 1987-04-11 | 1988-04-08 | Genteknologisk fremgangsmaate for fremstilling av polypeptider. |
| IL86023A IL86023A (en) | 1987-04-11 | 1988-04-10 | Genetic engineering process for the preparation of polypeptides containing -galactosidase or a' -galactosidase fragment' |
| DK196188A DK196188A (da) | 1987-04-11 | 1988-04-11 | Fremgangsmaade til fremstilling af et polypeptid, der kan kodes for genetisk, og anvendelse heraf |
| JP63088888A JPS63263087A (ja) | 1987-04-11 | 1988-04-11 | ポリペプチドの遺伝子工学的製造法 |
| KR1019880004072A KR880012758A (ko) | 1987-04-11 | 1988-04-11 | 폴리펩타이드의 유전공학적 제조방법 |
| PT87211A PT87211B (pt) | 1987-04-11 | 1988-04-11 | Processo para a preparacao de polipeptideos por manipulacao genetica |
| AU14469/88A AU603883B2 (en) | 1987-04-11 | 1988-04-11 | Genetic engineering process for the preparation of polypeptides |
| GR930400200T GR3007417T3 (cg-RX-API-DMAC7.html) | 1987-04-11 | 1993-03-19 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3712361 | 1987-04-11 | ||
| DE3805150A DE3805150A1 (de) | 1987-04-11 | 1988-02-19 | Gentechnologisches verfahren zur herstellung von polypeptiden |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3805150A1 true DE3805150A1 (de) | 1988-10-20 |
Family
ID=25854588
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE3805150A Withdrawn DE3805150A1 (de) | 1987-04-11 | 1988-02-19 | Gentechnologisches verfahren zur herstellung von polypeptiden |
| DE8888105438T Expired - Fee Related DE3877035D1 (de) | 1987-04-11 | 1988-04-06 | Gentechnologisches verfahren zur herstellung von polypeptiden. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE8888105438T Expired - Fee Related DE3877035D1 (de) | 1987-04-11 | 1988-04-06 | Gentechnologisches verfahren zur herstellung von polypeptiden. |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP0286956B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JPS63263087A (cg-RX-API-DMAC7.html) |
| KR (1) | KR880012758A (cg-RX-API-DMAC7.html) |
| AU (1) | AU603883B2 (cg-RX-API-DMAC7.html) |
| DE (2) | DE3805150A1 (cg-RX-API-DMAC7.html) |
| DK (1) | DK196188A (cg-RX-API-DMAC7.html) |
| FI (1) | FI881617A7 (cg-RX-API-DMAC7.html) |
| GR (1) | GR3007417T3 (cg-RX-API-DMAC7.html) |
| IL (1) | IL86023A (cg-RX-API-DMAC7.html) |
| NO (1) | NO881548L (cg-RX-API-DMAC7.html) |
| NZ (1) | NZ224184A (cg-RX-API-DMAC7.html) |
| PT (1) | PT87211B (cg-RX-API-DMAC7.html) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK175535B1 (da) * | 1987-03-04 | 2004-11-29 | Daiichi Suntory Pharma Co Ltd | Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid |
| DE3716722A1 (de) * | 1987-05-19 | 1988-12-01 | Hoechst Ag | Gentechnologisches verfahren zur herstellung von angiogeninen |
| DE3844211A1 (de) * | 1988-12-29 | 1990-07-05 | Hoechst Ag | Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung |
| US5179196A (en) * | 1989-05-04 | 1993-01-12 | Sri International | Purification of proteins employing ctap-iii fusions |
| US5527687A (en) * | 1993-05-25 | 1996-06-18 | American Cyanamid Company | Enzyme induction screen for ergosterol biosynthesis inhibitors |
| CA2198966C (en) * | 1996-03-04 | 2011-06-21 | Yuji Suzuki | Method for cleaving chimeric protein using processing enzyme |
| PT821006E (pt) | 1996-07-26 | 2004-09-30 | Aventis Pharma Gmbh | Derivados de insulina com ligacao a zinco aumentada |
| DE19825447A1 (de) | 1998-06-06 | 1999-12-09 | Hoechst Marion Roussel De Gmbh | Neue Insulinanaloga mit erhöhter Zinkbildung |
| CN105189788B (zh) * | 2013-03-12 | 2018-07-13 | 纳幕尔杜邦公司 | 用于鉴定罕见切割工程化双链断裂诱导剂的变体识别位点的方法及其组合物和用途 |
| KR20200080748A (ko) | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법 |
| KR20200080747A (ko) | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2008123B (en) * | 1977-11-08 | 1982-09-08 | Genentech Inc | Method of means for microbial polypeptide expression |
| JPS6028994A (ja) * | 1983-07-08 | 1985-02-14 | Wakunaga Seiyaku Kk | 〔２１―ロイシン〕ヒトウロガストロン |
| WO1985000831A1 (en) * | 1983-08-10 | 1985-02-28 | Amgen | Microbial expression of insulin-like growth factor |
| DK175535B1 (da) * | 1987-03-04 | 2004-11-29 | Daiichi Suntory Pharma Co Ltd | Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid |
| DE3715033A1 (de) * | 1987-05-06 | 1988-11-17 | Hoechst Ag | Verfahren zur isolierung von fusionsproteinen |
-
1988
- 1988-02-19 DE DE3805150A patent/DE3805150A1/de not_active Withdrawn
- 1988-04-06 DE DE8888105438T patent/DE3877035D1/de not_active Expired - Fee Related
- 1988-04-06 EP EP88105438A patent/EP0286956B1/de not_active Expired - Lifetime
- 1988-04-07 FI FI881617A patent/FI881617A7/fi not_active IP Right Cessation
- 1988-04-08 NZ NZ224184A patent/NZ224184A/xx unknown
- 1988-04-08 NO NO881548A patent/NO881548L/no unknown
- 1988-04-10 IL IL86023A patent/IL86023A/xx unknown
- 1988-04-11 AU AU14469/88A patent/AU603883B2/en not_active Ceased
- 1988-04-11 JP JP63088888A patent/JPS63263087A/ja active Pending
- 1988-04-11 DK DK196188A patent/DK196188A/da not_active Application Discontinuation
- 1988-04-11 KR KR1019880004072A patent/KR880012758A/ko not_active Ceased
- 1988-04-11 PT PT87211A patent/PT87211B/pt not_active IP Right Cessation
-
1993
- 1993-03-19 GR GR930400200T patent/GR3007417T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PT87211B (pt) | 1992-07-31 |
| FI881617A7 (fi) | 1988-10-12 |
| FI881617A0 (fi) | 1988-04-07 |
| DK196188A (da) | 1988-10-12 |
| KR880012758A (ko) | 1988-11-29 |
| EP0286956B1 (de) | 1992-12-30 |
| NO881548L (no) | 1988-10-12 |
| EP0286956A3 (en) | 1989-04-05 |
| GR3007417T3 (cg-RX-API-DMAC7.html) | 1993-07-30 |
| AU1446988A (en) | 1988-10-13 |
| EP0286956A2 (de) | 1988-10-19 |
| PT87211A (pt) | 1988-05-01 |
| DE3877035D1 (de) | 1993-02-11 |
| NZ224184A (en) | 1989-10-27 |
| NO881548D0 (no) | 1988-04-08 |
| IL86023A0 (en) | 1988-09-30 |
| IL86023A (en) | 1993-01-31 |
| JPS63263087A (ja) | 1988-10-31 |
| DK196188D0 (da) | 1988-04-11 |
| AU603883B2 (en) | 1990-11-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8130 | Withdrawal |