DE3786306D1 - Chimaere enzyme. - Google Patents

Chimaere enzyme.

Info

Publication number
DE3786306D1
DE3786306D1 DE8787305781T DE3786306T DE3786306D1 DE 3786306 D1 DE3786306 D1 DE 3786306D1 DE 8787305781 T DE8787305781 T DE 8787305781T DE 3786306 T DE3786306 T DE 3786306T DE 3786306 D1 DE3786306 D1 DE 3786306D1
Authority
DE
Germany
Prior art keywords
amino acid
acid residues
terminal
alpha
amylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8787305781T
Other languages
English (en)
Other versions
DE3786306T2 (de
Inventor
Boerge Krag Diderichsen
Helle Outtrup
Martin Schuelein
Barrie Edmund Norman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Industri AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Industri AS filed Critical Novo Industri AS
Application granted granted Critical
Publication of DE3786306D1 publication Critical patent/DE3786306D1/de
Publication of DE3786306T2 publication Critical patent/DE3786306T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DE87305781T 1986-06-30 1987-06-30 Chimäre Enzyme. Expired - Lifetime DE3786306T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK311186A DK311186D0 (da) 1986-06-30 1986-06-30 Enzymer

Publications (2)

Publication Number Publication Date
DE3786306D1 true DE3786306D1 (de) 1993-07-29
DE3786306T2 DE3786306T2 (de) 1994-01-13

Family

ID=8119209

Family Applications (1)

Application Number Title Priority Date Filing Date
DE87305781T Expired - Lifetime DE3786306T2 (de) 1986-06-30 1987-06-30 Chimäre Enzyme.

Country Status (6)

Country Link
EP (1) EP0252666B1 (de)
JP (1) JP2582787B2 (de)
AT (1) ATE90965T1 (de)
DE (1) DE3786306T2 (de)
DK (1) DK311186D0 (de)
ES (1) ES2056821T3 (de)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD282028A5 (de) * 1989-02-16 1990-08-29 Akad Wissenschaften Ddr Verfahren zur herstellung einer thermostabilen, hybriden bacillus-beta-1,3-1,4-glukanase
ES2092504T3 (es) * 1989-02-16 1996-12-01 Carlsberg As (1,3-1,4)-beta-glucanasa termoestable.
DE69032360T2 (de) * 1989-06-29 1998-12-03 Genencor International, Inc., Rochester, N.Y. Mutierte mikrobielle alpha-Amylasen mit erhöhter thermischer, saurer und/oder alkylischer Stabilität
US5830837A (en) * 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
WO1995010603A1 (en) * 1993-10-08 1995-04-20 Novo Nordisk A/S Amylase variants
EP2199386A1 (de) 1993-10-08 2010-06-23 Novozymes A/S Amylasevarianten
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
ES2432519T3 (es) 1996-04-30 2013-12-04 Novozymes A/S Mutantes de alfa-amilasa
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
FR2762616B1 (fr) * 1997-04-28 1999-07-16 Roquette Freres Procede de fabrication d'un hydrolysat d'amidon a haute teneur en dextrose
ATE423192T1 (de) 1997-10-13 2009-03-15 Novozymes As Mutanten der alpha-amylase
CA2308119C (en) 1997-10-30 2014-06-03 Novo Nordisk A/S .alpha.-amylase mutants
FR2770854B1 (fr) * 1997-11-07 2001-11-30 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un tel gene, tolerantes aux herbicides
JP3712255B2 (ja) 1997-12-08 2005-11-02 カリフォルニア・インスティチュート・オブ・テクノロジー ポリヌクレオチドおよびポリペプチド配列を生成するための方法
EP1062359A1 (de) * 1998-03-09 2000-12-27 Novo Nordisk A/S Enzymatische aufarbeitung von glucosesirup aus stärke
CA2331199C (en) 1998-06-10 2012-10-23 Markus Sakari Kauppinen Isolated mannanases for use in treating cellulosic or synthetic fibers
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
CN1252256C (zh) 1999-03-30 2006-04-19 诺维信公司 α－淀粉酶变体
CN101532000A (zh) 2000-03-08 2009-09-16 诺维信公司 具有改变的特性的变体
US6768044B1 (en) 2000-05-10 2004-07-27 Bayer Cropscience Sa Chimeric hydroxyl-phenyl pyruvate dioxygenase, DNA sequence and method for obtaining plants containing such a gene, with herbicide tolerance
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
CN101857858A (zh) 2000-08-01 2010-10-13 诺维信公司 具有改变特性的α-淀粉酶突变体
US20040091994A1 (en) 2000-10-13 2004-05-13 Carsten Andersen Alpha-amylase variant with altered properties
US7659102B2 (en) 2001-02-21 2010-02-09 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
US7273740B2 (en) 2001-02-21 2007-09-25 Verenium Corporation Enzymes having alpha amylase activity and methods of use thereof
US7498158B2 (en) 2001-05-15 2009-03-03 Novozymes A/S Alpha-amylase variant with altered properties
US7741092B2 (en) 2002-10-31 2010-06-22 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
WO2006002643A2 (en) 2004-07-05 2006-01-12 Novozymes A/S Alpha-amylase variants with altered properties
CA2704745C (en) 2007-11-05 2019-01-15 Danisco Us Inc. Alpha-amylase variants with altered properties
NZ584434A (en) 2007-11-05 2011-12-22 Danisco Us Inc VARIANTS OF BACILLUS sp. TS-23 ALPHA-AMYLASE WITH ALTERED PROPERTIES
US8236545B2 (en) 2008-02-04 2012-08-07 Danisco Us Inc., Genencor Division TS23 alpha-amylase variants with altered properties
US8084240B2 (en) 2008-06-06 2011-12-27 Danisco Us Inc. Geobacillus stearothermophilus α-amylase (AmyS) variants with improved properties
BRPI0920891B1 (pt) 2008-09-25 2023-01-10 Danisco Us Inc Mistura de alfa-amilase e método para produção de um açúcar fermentável
WO2011076897A1 (en) 2009-12-22 2011-06-30 Novozymes A/S Use of amylase variants at low temperature
US8435577B2 (en) 2010-01-04 2013-05-07 Novozymes A/S Alpha-amylases
EP2357220A1 (de) 2010-02-10 2011-08-17 The Procter & Gamble Company Reinigungszusammensetzungen mit Amylasevarianten mit hoher Stabilität in Gegenwart eines Chelatwirkstoffs
CN113186178A (zh) 2010-02-10 2021-07-30 诺维信公司 在螯合剂存在下具有高稳定性的变体和包含变体的组合物
EP2726607B1 (de) 2011-06-30 2018-08-08 Novozymes A/S Alpha-amylase-varianten
US20240110206A1 (en) * 2020-10-13 2024-04-04 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0134048B2 (de) * 1983-07-06 1996-08-14 Gist-Brocades N.V. Molekulare Klonierung und Expression in industriellen Mikroorganismen
JPS61115496A (ja) * 1984-11-09 1986-06-03 Hitachi Ltd 殿粉の液化方法
EP0208491B1 (de) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybride Polypeptide und Verfahren zu deren Herstellung

Also Published As

Publication number Publication date
JP2582787B2 (ja) 1997-02-19
ATE90965T1 (de) 1993-07-15
DE3786306T2 (de) 1994-01-13
EP0252666A2 (de) 1988-01-13
EP0252666A3 (en) 1989-03-15
ES2056821T3 (es) 1994-10-16
DK311186D0 (da) 1986-06-30
JPS6368084A (ja) 1988-03-26
EP0252666B1 (de) 1993-06-23

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