DE3774826D1 - Abbildungsgeraet durch fluoreszenz mit mehreren abfallzeiten und verfahren zur ortsaufloesung damit. - Google Patents

Abbildungsgeraet durch fluoreszenz mit mehreren abfallzeiten und verfahren zur ortsaufloesung damit.

Info

Publication number
DE3774826D1
DE3774826D1 DE8787420132T DE3774826T DE3774826D1 DE 3774826 D1 DE3774826 D1 DE 3774826D1 DE 8787420132 T DE8787420132 T DE 8787420132T DE 3774826 T DE3774826 T DE 3774826T DE 3774826 D1 DE3774826 D1 DE 3774826D1
Authority
DE
Germany
Prior art keywords
fluorescence
locating
image device
several waste
waste times
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8787420132T
Other languages
English (en)
Inventor
Arnold Honig
James Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syracuse University
Original Assignee
Syracuse University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syracuse University filed Critical Syracuse University
Application granted granted Critical
Publication of DE3774826D1 publication Critical patent/DE3774826D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/178—Methods for obtaining spatial resolution of the property being measured
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G01N2021/641—Phosphorimetry, gated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G01N2021/6413—Distinction short and delayed fluorescence or phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/064—Stray light conditioning
    • G01N2201/0648—Shutters

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
DE8787420132T 1986-10-02 1987-05-19 Abbildungsgeraet durch fluoreszenz mit mehreren abfallzeiten und verfahren zur ortsaufloesung damit. Expired - Fee Related DE3774826D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/914,367 US4791310A (en) 1986-10-02 1986-10-02 Fluorescence microscopy

Publications (1)

Publication Number Publication Date
DE3774826D1 true DE3774826D1 (de) 1992-01-09

Family

ID=25434262

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8787420132T Expired - Fee Related DE3774826D1 (de) 1986-10-02 1987-05-19 Abbildungsgeraet durch fluoreszenz mit mehreren abfallzeiten und verfahren zur ortsaufloesung damit.

Country Status (4)

Country Link
US (1) US4791310A (de)
EP (1) EP0263037B1 (de)
JP (1) JPS63204138A (de)
DE (1) DE3774826D1 (de)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4877965A (en) * 1985-07-01 1989-10-31 Diatron Corporation Fluorometer
USRE34782E (en) * 1985-07-01 1994-11-08 Diatron Corporation Fluorometer
JP2749069B2 (ja) * 1988-04-26 1998-05-13 オリンパス光学工業株式会社 蛍光顕微鏡装置
US4948975A (en) * 1988-09-08 1990-08-14 The United States Of America As Represented By The Secretary Of The Air Force Quantitative luminescence imaging system
US5061076A (en) * 1989-01-31 1991-10-29 Enzo Diagnostics, Inc. Time-resolved fluorometer
JPH083481B2 (ja) * 1989-06-07 1996-01-17 日立ソフトウェアエンジニアリング株式会社 蛍光式電気泳動パターン読み取り装置
US5034613A (en) * 1989-11-14 1991-07-23 Cornell Research Foundation, Inc. Two-photon laser microscopy
US5091652A (en) * 1990-01-12 1992-02-25 The Regents Of The University Of California Laser excited confocal microscope fluorescence scanner and method
US5274240A (en) * 1990-01-12 1993-12-28 The Regents Of The University Of California Capillary array confocal fluorescence scanner and method
US5149972A (en) * 1990-01-18 1992-09-22 University Of Massachusetts Medical Center Two excitation wavelength video imaging microscope
US5487112A (en) * 1990-02-20 1996-01-23 Board Of Regents, The University Of Texas System Method and apparatus for time-resolved measurements of lymphocyte function and aggregate structure using computer-automated microscopy
US5127730A (en) * 1990-08-10 1992-07-07 Regents Of The University Of Minnesota Multi-color laser scanning confocal imaging system
US5252834A (en) * 1990-11-13 1993-10-12 Union Oil Company Of California Pulsed and gated multi-mode microspectrophotometry device and method
WO1992013265A1 (en) * 1991-01-24 1992-08-06 The University Of Maryland Method and apparatus for multi-dimensional phase fluorescence lifetime imaging
DE4210970C2 (de) * 1992-04-02 1996-10-17 Markus Dipl Chem Sauer Verfahren zur simultanen optischen qualitativen und quantitativen Erfassung von verschiedenen mit Fluorochromen oder Fluorogenen markierten Molekülen eines Gemisches mittels Laserspektroskopie
FI96452C (fi) * 1994-01-26 1996-06-25 Pekka Haenninen Menetelmä väriaineiden virittämiseksi
JP3448090B2 (ja) * 1994-02-16 2003-09-16 浜松ホトニクス株式会社 エネルギー移動検出法およびその装置
US6074870A (en) * 1994-08-15 2000-06-13 Becton, Dickinson And Company Optical blood culture sensor
US6166385A (en) * 1995-09-19 2000-12-26 Cornell Research Foundation, Inc. Multi-photon laser microscopy
US5861618A (en) * 1995-10-23 1999-01-19 Pitney Bowes, Inc. System and method of improving the signal to noise ratio of bar code and indicia scanners that utilize fluorescent inks
US5784157A (en) * 1995-11-21 1998-07-21 The Research Foundation Of State University Of New York Method and apparatus for identifying fluorophores
ATE366418T1 (de) 1996-04-25 2007-07-15 Bioarray Solutions Ltd Licht-regulierte, elektrokinetische zusammensetzung von partikeln an oberflächen
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US6650357B1 (en) * 1997-04-09 2003-11-18 Richardson Technologies, Inc. Color translating UV microscope
DE19718016A1 (de) * 1997-04-29 1998-11-05 Drexhage Karl Heinz Verfahren zur optischen Detektion von Analytmolekülen in einem natürlichen biologischen Medium
US6264107B1 (en) 1997-09-26 2001-07-24 Iomega Corporation Latent illuminance discrimination marker system for authenticating articles
US6091563A (en) * 1997-09-26 2000-07-18 Iomega Corporation Latent illuminance discrimination marker system for data storage cartridges
US6181662B1 (en) 1997-09-26 2001-01-30 Iomega Corporation Latent irradiance discrimination method and marker system for cartridgeless data storage disks
US6201662B1 (en) 1998-09-25 2001-03-13 Iomega Corporation Latent illuminance discrimination marker with reflective layer for data storage cartridges
US6359745B1 (en) 1997-09-26 2002-03-19 Iomega Corporation Latent illuminance discrimination marker system for data storage cartridges
GB2350005B (en) * 1999-03-17 2003-01-08 Canon Kk Image processing apparatus
DE19915137C2 (de) * 1999-03-26 2001-10-18 Michael Schaefer Verfahren zur Quantifizierung mehrerer Fluorochrome in einer mehrfach gefärbten Probe bei der Fluoreszenzmikroskopie und Verwendungen des Verfahrens
US6215586B1 (en) * 1999-05-25 2001-04-10 R.K.C. Technologies Inc. Active optical image enhancer for a microscope
ES2642930T3 (es) * 2000-04-11 2017-11-20 Chemometec A/S Procedimiento y aparato para detectar fluorescencia de una muestra
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
DE60117556T2 (de) 2000-06-21 2006-11-02 Bioarray Solutions Ltd. Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays
WO2002019594A2 (en) * 2000-08-02 2002-03-07 Arizona Board Of Regents, Acting On Behalf Of Arizona State University Scanning fluorescence lifetime microscope: directed evolution
GB0023619D0 (en) * 2000-09-27 2000-11-08 Amersham Pharm Biotech Uk Ltd Measurement of fluorescence decay times
US20030045005A1 (en) 2000-10-17 2003-03-06 Michael Seul Light-controlled electrokinetic assembly of particles near surfaces
DE10054426B4 (de) * 2000-10-27 2006-03-09 Iom Innovative Optische Messtechnik Gmbh Verfahren zur Multi-Fluoreszenz-Detektion
US6694158B2 (en) 2001-04-11 2004-02-17 Motorola, Inc. System using a portable detection device for detection of an analyte through body tissue
US6454710B1 (en) 2001-04-11 2002-09-24 Motorola, Inc. Devices and methods for monitoring an analyte
US6379622B1 (en) 2001-04-11 2002-04-30 Motorola, Inc. Sensor incorporating a quantum dot as a reference
US7521019B2 (en) * 2001-04-11 2009-04-21 Lifescan, Inc. Sensor device and methods for manufacture
US7015484B2 (en) * 2001-04-16 2006-03-21 Dakota Technologies, Inc. Multi-dimensional fluorescence apparatus and method for rapid and highly sensitive quantitative analysis of mixtures
US20020158211A1 (en) * 2001-04-16 2002-10-31 Dakota Technologies, Inc. Multi-dimensional fluorescence apparatus and method for rapid and highly sensitive quantitative analysis of mixtures
JP3741051B2 (ja) * 2001-05-10 2006-02-01 横河電機株式会社 バイオチップ読取装置
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US20040002073A1 (en) 2001-10-15 2004-01-01 Li Alice Xiang Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
US7285424B2 (en) 2002-08-27 2007-10-23 Kimberly-Clark Worldwide, Inc. Membrane-based assay devices
AU2003298655A1 (en) 2002-11-15 2004-06-15 Bioarray Solutions, Ltd. Analysis, secure access to, and transmission of array images
GB2404013B (en) * 2003-07-17 2006-05-31 Isis Innovation Apparatus for and method of measuring fluorescence lifetime
US7927796B2 (en) 2003-09-18 2011-04-19 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
PT1664722E (pt) 2003-09-22 2011-12-28 Bioarray Solutions Ltd Polielectrólito imobilizado à superfície com grupos funcionais múltiplos capazes de se ligarem covalentemente às biomoléculas
US7563569B2 (en) 2003-10-28 2009-07-21 Michael Seul Optimization of gene expression analysis using immobilized capture probes
ES2533876T3 (es) 2003-10-29 2015-04-15 Bioarray Solutions Ltd Análisis multiplexado de ácidos nucleicos mediante fragmentación de ADN bicatenario
US20050112703A1 (en) 2003-11-21 2005-05-26 Kimberly-Clark Worldwide, Inc. Membrane-based lateral flow assay devices that utilize phosphorescent detection
US7943395B2 (en) 2003-11-21 2011-05-17 Kimberly-Clark Worldwide, Inc. Extension of the dynamic detection range of assay devices
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
US20070191675A1 (en) * 2006-02-13 2007-08-16 Joel Gerardo Diaz Sanchez Actinic light colposcope and method to detect lesions in the lower female genital tract produced by human papilloma virus using an actinic light colposcope
WO2008072365A1 (ja) * 2006-12-13 2008-06-19 Nikon Corporation 蛍光検出装置及び蛍光観察システム
DE102009029831A1 (de) 2009-06-17 2011-01-13 W.O.M. World Of Medicine Ag Vorrichtung und Verfahren für die Mehr-Photonen-Fluoreszenzmikroskopie zur Gewinnung von Informationen aus biologischem Gewebe
CH701812A1 (de) 2009-09-10 2011-03-15 Alstom Technology Ltd Verwendung von Laser-optischen Vorrichtungen.
US9091151B2 (en) * 2009-11-19 2015-07-28 Halliburton Energy Services, Inc. Downhole optical radiometry tool
US20130087719A1 (en) * 2010-08-13 2013-04-11 Lumos Technology Co., Ltd. Light source device for time-delayed detection of fluorescence, and image pick-up system and method
AU2013225655A1 (en) 2012-03-02 2014-09-18 The Regents Of The University Of California System and method for time-resolved fluorescence imaging and pulse shaping
US20130258343A1 (en) * 2012-03-30 2013-10-03 Agilent Technologies, Inc. Method and apparatus to improve signal-to-noise ratio of ft-ir spectrometers using pulsed light source
US8977331B2 (en) 2012-12-13 2015-03-10 General Electric Company Systems and methods for nerve imaging
DE102013226777B4 (de) * 2013-12-19 2023-01-12 Sirona Dental Systems Gmbh Beobachtungseinheit zur Erkennung von Fluoreszenz und eine Vorrichtung zur Erkennung von Karies
US9645291B1 (en) 2016-04-18 2017-05-09 Ii-Vi Incorporated Voltage-tunable optical filters for instrumentation applications
JOP20190168A1 (ar) * 2017-06-14 2019-07-02 Grow Solutions Tech Llc أنظمة وطرق لالتقاط صورة بحجيرة نمو خط تجميع
DE102017121483B3 (de) * 2017-09-15 2019-03-07 Leica Microsystems Cms Gmbh Verfahren zur Untersuchung einer multispektralen Probe, Steuereinheit hierfür und Mikroskop-Anordnung
JP7064315B2 (ja) * 2017-11-07 2022-05-10 オリンパス株式会社 光検出装置およびレーザ走査型顕微鏡
CN110434463B (zh) * 2019-08-23 2021-04-27 上海第一机床厂有限公司 辐照样品架的激光焊接方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4006360A (en) * 1974-08-21 1977-02-01 Block Engineering, Inc. Method of discriminating between dyed particles and background fluorescence of the dye
US4058732A (en) * 1975-06-30 1977-11-15 Analytical Radiation Corporation Method and apparatus for improved analytical fluorescent spectroscopy
US4236071A (en) * 1979-05-21 1980-11-25 Exxon Research & Engineering Co. Detection of uranium by light induced luminescence
US4626693A (en) * 1980-10-06 1986-12-02 The Regents Of The University Of California Remote multi-position information gathering system and method
GB2095822B (en) * 1981-03-30 1984-10-10 Ramley Engineering Co Ltd Identifying objects by detecting decaying phosphorescence from phosphor coating thereon
JPS5841337A (ja) * 1981-09-04 1983-03-10 Hamamatsu Tv Kk 発光現象の測定装置
FR2532756A1 (fr) * 1982-09-03 1984-03-09 France Henri De Systeme pour l'observation et la quantification automatiques de phenomenes susceptibles d'etre detectes par fluorescence
US4573796A (en) * 1984-01-06 1986-03-04 The United States Of America As Represented By The United States Department Of Energy Apparatus for eliminating background interference in fluorescence measurements

Also Published As

Publication number Publication date
US4791310A (en) 1988-12-13
EP0263037B1 (de) 1991-11-27
JPS63204138A (ja) 1988-08-23
EP0263037A2 (de) 1988-04-06
EP0263037A3 (en) 1989-07-26

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