DE3738982A1 - Verfahren zur bestimmung von proteinen, proteinstrukturen und nukleinsaeuren aus koerperfluessigkeiten - Google Patents
Verfahren zur bestimmung von proteinen, proteinstrukturen und nukleinsaeuren aus koerperfluessigkeitenInfo
- Publication number
- DE3738982A1 DE3738982A1 DE19873738982 DE3738982A DE3738982A1 DE 3738982 A1 DE3738982 A1 DE 3738982A1 DE 19873738982 DE19873738982 DE 19873738982 DE 3738982 A DE3738982 A DE 3738982A DE 3738982 A1 DE3738982 A1 DE 3738982A1
- Authority
- DE
- Germany
- Prior art keywords
- proteins
- hydrophobic
- nucleic acids
- recesses
- film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 42
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 36
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 36
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 20
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 20
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 20
- 210000001124 body fluid Anatomy 0.000 title claims abstract description 12
- 239000010839 body fluid Substances 0.000 title claims abstract description 12
- 239000002250 absorbent Substances 0.000 claims abstract description 32
- 230000002745 absorbent Effects 0.000 claims abstract description 32
- 239000000654 additive Substances 0.000 claims abstract description 19
- 230000000996 additive effect Effects 0.000 claims abstract description 17
- 238000002405 diagnostic procedure Methods 0.000 claims abstract description 4
- 239000002855 microbicide agent Substances 0.000 claims abstract description 3
- 210000004369 blood Anatomy 0.000 claims description 41
- 230000002209 hydrophobic effect Effects 0.000 claims description 23
- 239000000463 material Substances 0.000 claims description 23
- 230000000087 stabilizing effect Effects 0.000 claims description 15
- 239000010408 film Substances 0.000 claims description 13
- 229930006000 Sucrose Natural products 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 239000004033 plastic Substances 0.000 claims description 7
- 239000005720 sucrose Substances 0.000 claims description 7
- 235000000346 sugar Nutrition 0.000 claims description 7
- 230000001413 cellular effect Effects 0.000 claims description 6
- 239000011888 foil Substances 0.000 claims description 6
- 238000002965 ELISA Methods 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 239000013039 cover film Substances 0.000 claims description 4
- 150000008163 sugars Chemical class 0.000 claims description 4
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims description 3
- 230000028327 secretion Effects 0.000 claims description 3
- 210000002700 urine Anatomy 0.000 claims description 3
- 101710172711 Structural protein Proteins 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 238000003018 immunoassay Methods 0.000 claims description 2
- 238000010820 immunofluorescence microscopy Methods 0.000 claims description 2
- 229920001542 oligosaccharide Polymers 0.000 claims description 2
- 150000002482 oligosaccharides Chemical class 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000002798 spectrophotometry method Methods 0.000 claims description 2
- 239000008280 blood Substances 0.000 description 40
- 238000012360 testing method Methods 0.000 description 21
- 239000000243 solution Substances 0.000 description 18
- 239000000523 sample Substances 0.000 description 17
- 210000002966 serum Anatomy 0.000 description 10
- 241000725303 Human immunodeficiency virus Species 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 9
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 8
- 238000010828 elution Methods 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 238000010166 immunofluorescence Methods 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 239000002313 adhesive film Substances 0.000 description 5
- 230000036436 anti-hiv Effects 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 239000000969 carrier Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 208000030507 AIDS Diseases 0.000 description 3
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000013024 dilution buffer Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 208000031886 HIV Infections Diseases 0.000 description 1
- 208000037357 HIV infectious disease Diseases 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 235000019797 dipotassium phosphate Nutrition 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010206 sensitivity analysis Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000005353 urine analysis Methods 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/521—Single-layer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
- G01N33/567—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds utilising isolate of tissue or organ as binding agent
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19873738982 DE3738982A1 (de) | 1987-04-30 | 1987-11-17 | Verfahren zur bestimmung von proteinen, proteinstrukturen und nukleinsaeuren aus koerperfluessigkeiten |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3714445 | 1987-04-30 | ||
| DE19873738982 DE3738982A1 (de) | 1987-04-30 | 1987-11-17 | Verfahren zur bestimmung von proteinen, proteinstrukturen und nukleinsaeuren aus koerperfluessigkeiten |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3738982A1 true DE3738982A1 (de) | 1988-11-17 |
| DE3738982C2 DE3738982C2 (enExample) | 1989-11-23 |
Family
ID=25855109
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19873738982 Granted DE3738982A1 (de) | 1987-04-30 | 1987-11-17 | Verfahren zur bestimmung von proteinen, proteinstrukturen und nukleinsaeuren aus koerperfluessigkeiten |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3738982A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1102102A3 (en) * | 1999-11-22 | 2002-09-25 | Genpak Limited | Adhesive label with grid for microscope slide |
| DE102006009534A1 (de) * | 2006-02-28 | 2007-08-30 | Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH | Verfahren zum Aufbewahren des humanen Wachstumshormons Somatotropin und Verfahren zum quantitativen Nachweis des humanen Wachstumshormons Somatotropin |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19952139C1 (de) * | 1999-10-28 | 2000-12-21 | Sibyll Weiner | Transparenter Objektträger für die optische Mikroskopie |
| DE102010005622A1 (de) * | 2010-01-25 | 2011-12-15 | Umweltmykologie Dr. Dill & Trautmann Gbr (Vertreten Durch Den Gesellschafter: Dr.Trautmann, 10961 Berlin) | Folien - Test |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2015271A1 (de) * | 1969-04-01 | 1970-10-15 | Miles Laboratories Inc., Elkhart, Ind. (V.St.A.) | Prüfmittel zum Nachweis geringer Konzentrationen von Substanzen in Flüssigkeiten |
| DE1773841B1 (de) * | 1968-07-15 | 1971-12-09 | Boehringer Mannheim Gmbh | Verfahren zur Vorbereitung,Konservierung und zum Transport von Blut- und Serumproben |
| DE2555704A1 (de) * | 1974-12-18 | 1976-06-24 | Becton Dickinson Co | Indikator zur quantitativen feststellung von in biologischen fluessigkeiten geloesten stoffen |
| DE2424426B2 (de) * | 1973-06-12 | 1977-02-10 | Gasbarro, Luciano, Dr., Florenz (Italien) | Palette zur aufnahme einer vielzahl von biologischen fluessigkeitsproben |
-
1987
- 1987-11-17 DE DE19873738982 patent/DE3738982A1/de active Granted
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1773841B1 (de) * | 1968-07-15 | 1971-12-09 | Boehringer Mannheim Gmbh | Verfahren zur Vorbereitung,Konservierung und zum Transport von Blut- und Serumproben |
| DE2015271A1 (de) * | 1969-04-01 | 1970-10-15 | Miles Laboratories Inc., Elkhart, Ind. (V.St.A.) | Prüfmittel zum Nachweis geringer Konzentrationen von Substanzen in Flüssigkeiten |
| DE2424426B2 (de) * | 1973-06-12 | 1977-02-10 | Gasbarro, Luciano, Dr., Florenz (Italien) | Palette zur aufnahme einer vielzahl von biologischen fluessigkeitsproben |
| DE2555704A1 (de) * | 1974-12-18 | 1976-06-24 | Becton Dickinson Co | Indikator zur quantitativen feststellung von in biologischen fluessigkeiten geloesten stoffen |
Non-Patent Citations (2)
| Title |
|---|
| Hunnius, Pharmazeutisches W�rterbuch, 6. Aufl., Berlin 1986, S. 913 u. 914 * |
| Lexikon der Pharmazie, herausgeg. von S. Ebel u. H.J. Roth, Stuttgart 1987, S. 574 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1102102A3 (en) * | 1999-11-22 | 2002-09-25 | Genpak Limited | Adhesive label with grid for microscope slide |
| US6597500B1 (en) | 1999-11-22 | 2003-07-22 | Genetix Limited | Adhesive label with grid for microscope slide |
| DE102006009534A1 (de) * | 2006-02-28 | 2007-08-30 | Mediagnost Gesellschaft für Forschung und Herstellung von Diagnostika GmbH | Verfahren zum Aufbewahren des humanen Wachstumshormons Somatotropin und Verfahren zum quantitativen Nachweis des humanen Wachstumshormons Somatotropin |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3738982C2 (enExample) | 1989-11-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8181 | Inventor (new situation) |
Free format text: IMMELMANN, ANDREAS, DR. HENCO, KARSTEN, DR., 4006 ERKRATH, DE |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |