DE3689572D1 - Verfahren zur Isolierung von Genen zur Biosynthese von Antibiotika. - Google Patents

Verfahren zur Isolierung von Genen zur Biosynthese von Antibiotika.

Info

Publication number
DE3689572D1
DE3689572D1 DE86304239T DE3689572T DE3689572D1 DE 3689572 D1 DE3689572 D1 DE 3689572D1 DE 86304239 T DE86304239 T DE 86304239T DE 3689572 T DE3689572 T DE 3689572T DE 3689572 D1 DE3689572 D1 DE 3689572D1
Authority
DE
Germany
Prior art keywords
biosynthesis
antibiotics
genes
isolation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE86304239T
Other languages
English (en)
Other versions
DE3689572T2 (de
Inventor
Ramachandra Nagaraja Rao
Richard Henry Baltz
Richard Kale Stanzak
Patti Jean Treadway
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of DE3689572D1 publication Critical patent/DE3689572D1/de
Publication of DE3689572T2 publication Critical patent/DE3689572T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
DE3689572T 1985-06-07 1986-06-04 Verfahren zur Isolierung von Genen zur Biosynthese von Antibiotika. Expired - Lifetime DE3689572T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/742,349 US4935340A (en) 1985-06-07 1985-06-07 Method of isolating antibiotic biosynthetic genes

Publications (2)

Publication Number Publication Date
DE3689572D1 true DE3689572D1 (de) 1994-03-10
DE3689572T2 DE3689572T2 (de) 1994-05-19

Family

ID=24984475

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3689572T Expired - Lifetime DE3689572T2 (de) 1985-06-07 1986-06-04 Verfahren zur Isolierung von Genen zur Biosynthese von Antibiotika.

Country Status (8)

Country Link
US (1) US4935340A (de)
EP (1) EP0204549B1 (de)
JP (1) JPS61285991A (de)
AU (2) AU5836786A (de)
DE (1) DE3689572T2 (de)
DK (1) DK259986A (de)
HU (1) HU202924B (de)
IL (1) IL79013A0 (de)

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US5024948A (en) * 1985-10-17 1991-06-18 American Cyanamid Company Genetic system for micromonospora
EP0233715B1 (de) * 1986-01-31 1994-05-25 BEECHAM GROUP plc Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen
US6709859B1 (en) 1986-01-31 2004-03-23 Beecham Group P.L.C. Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
US4889809A (en) * 1986-07-25 1989-12-26 Eli Lilly And Company Tylosin resistance-conferring gene, designated tlrC, for use in streptomyces fradiae
US4886757A (en) * 1987-04-15 1989-12-12 Eli Lilly And Company Spiramycin resistance-conferring cloning vectors
US5068189A (en) * 1988-05-13 1991-11-26 Eli Lilly And Company Recombinant dna vectors encoding a 4"-o-isovaleryl acylase derived from a carbomycin biosynthetic gene, designated care, for use in streptomyces and other organisms
GB8812697D0 (en) * 1988-05-27 1988-06-29 Erba Carlo Spa Isolation & characterisation of genes resistant to anthracycline antibiotics
US5665564A (en) * 1988-05-27 1997-09-09 Pharmacia & Upjohn S.P.A. Isolation and characterisation of genes resistant to anthracycline antibiotics
US5098837A (en) * 1988-06-07 1992-03-24 Eli Lilly And Company Macrolide biosynthetic genes for use in streptomyces and other organisms
JP2787458B2 (ja) * 1989-01-20 1998-08-20 旭化成工業株式会社 抗生物質ｌ５３―１８ａおよびその製造法
US5252474A (en) * 1989-03-31 1993-10-12 Merck & Co., Inc. Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
DK0399328T3 (da) * 1989-05-23 1995-12-11 Lepetit Spa Teico-resistens-tildelende sekvens
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
US6946587B1 (en) * 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
WO1991010725A1 (en) * 1990-01-22 1991-07-25 Dekalb Plant Genetics Fertile transgenic corn plants
US5484956A (en) * 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5589385A (en) * 1990-07-26 1996-12-31 American Cyanamid Company Cloning of the biosynthetic pathway for chlortetracycline and tetracycline formation and cosmids useful therein
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
US5824513A (en) * 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
US6060234A (en) * 1991-01-17 2000-05-09 Abbott Laboratories Polyketide derivatives and recombinant methods for making same
US6063561A (en) * 1991-01-17 2000-05-16 Abbott Laboratories Polyketide derivatives and recombinant methods for making same
WO1993013663A1 (en) * 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides
US5514544A (en) * 1991-07-26 1996-05-07 Eli Lilly And Company Activator gene for macrolide biosynthesis
DK0658201T3 (da) * 1992-08-31 2003-09-29 Syngenta Participations Ag DNA-sekvenser involveret i soraphen-biosyntese af myxobakterier
US6118047A (en) * 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
US5672491A (en) * 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
US6500960B1 (en) 1995-07-06 2002-12-31 Stanford University (Board Of Trustees Of The Leland Stanford Junior University) Method to produce novel polyketides
US6066721A (en) * 1995-07-06 2000-05-23 Stanford University Method to produce novel polyketides
US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
DK0725778T3 (da) * 1993-09-20 2001-12-17 Univ Leland Stanford Junior Rekombinant produktion af hidtil ukendte polyketider
US6558942B1 (en) 1994-05-06 2003-05-06 The Leland Stanford Junior University Combinatorial polyketide libraries produced using a modular PKS gene cluster as scaffold
US6524812B1 (en) 1993-10-07 2003-02-25 Regents Of The University Of Minnesota Genes encoding resistance to DNA alkylating agents
US6495348B1 (en) * 1993-10-07 2002-12-17 Regents Of The University Of Minnesota Mitomycin biosynthetic gene cluster
US6268140B1 (en) * 1994-02-03 2001-07-31 Neugenesis Combinatorial metabolic libraries
AU706445B2 (en) * 1995-07-06 1999-06-17 Brown University Research Foundation Cell-free synthesis of polyketides
WO1997022711A1 (en) 1995-12-19 1997-06-26 Regents Of The University Of Minnesota Metabolic engineering of polyhydroxyalkanoate monomer synthases
US6902913B2 (en) * 1997-04-30 2005-06-07 Kosan Biosciences, Inc. Recombinant narbonolide polyketide synthase
US6297007B1 (en) * 1997-05-22 2001-10-02 Terragen Diversity Inc. Method for isolation of biosynthesis genes for bioactive molecules
US6143526A (en) 1998-03-09 2000-11-07 Baltz; Richard H. Biosynthetic genes for spinosyn insecticide production
US6265202B1 (en) 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin
US6753173B1 (en) 1999-02-09 2004-06-22 Board Of Trustees Of The Leland Stanford Junior University Methods to mediate polyketide synthase module effectiveness
US7001748B2 (en) * 1999-02-09 2006-02-21 The Board Of Trustees Of The Leland Stanford Junior University Methods of making polyketides using hybrid polyketide synthases
WO2000062783A2 (en) 1999-04-16 2000-10-26 Ortho-Mcneil Pharmaceutical, Inc. Ketolide antibacterials
US6590083B1 (en) 1999-04-16 2003-07-08 Ortho-Mcneil Pharmaceutical, Inc. Ketolide antibacterials
AU2001290744A1 (en) * 2000-09-11 2002-03-26 University Of Rochester Method of identifying putative antibiotic resistance genes
US20030194784A1 (en) * 2001-04-17 2003-10-16 Sherman David H. DNA encoding methymycin and pikromycin
AU2003213757A1 (en) * 2002-03-04 2003-09-22 Brown University Research Foundation Methods to mediate polyketide synthase module effectiveness
WO2005021586A2 (en) * 2003-08-21 2005-03-10 Wisconsin Alumni Research Foundation Metabolic engineering of viomycin biosynthesis
DE102004017141A1 (de) * 2004-04-01 2005-12-01 Bergische Universität Wuppertal Neue Gene aus Biosynthese Genclustern zur Synthese von Aminoglycosid-Antibiotika sowie damit herstellbare neue Aminoglycosid-Antibiotika
WO2007107810A1 (en) * 2006-03-20 2007-09-27 Council Of Scientific And Industrial Research A method for detecting the presence of water born pathogens and indicator microorganism
AU2007304776A1 (en) * 2006-10-06 2008-04-10 Dna Genotek Inc. Stabilizing compositions and methods for extraction of ribonucleic acid
KR101445903B1 (ko) 2007-05-02 2014-09-29 메리얼 리미티드 향상된 발현 및 안정성을 갖는 ｄｎａ 플라스미드
EP2173880A2 (de) * 2007-07-27 2010-04-14 Wyeth a Corporation of the State of Delaware Vektoren und verfahren zum klonen von gen-clustern oder teilen davon

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4343906A (en) 1979-08-21 1982-08-10 The Upjohn Company Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same
US4340674A (en) 1980-05-05 1982-07-20 The Upjohn Company Cointegrate plasmids and their construction from plasmids of Escherichia and Streptomyces
US4362816A (en) 1980-11-13 1982-12-07 The Upjohn Company Hybrid plasmid and process of making same
US4477571A (en) 1981-02-09 1984-10-16 Cetus Corporation Chimeric plasmids that replicate in bacteria and yeast and microorganisms transformed therewith
US4416994A (en) 1981-10-19 1983-11-22 Eli Lilly And Company Plasmid pEL7 and related cloning vectors for use in streptomyces and related organisms
JPS6158589A (ja) * 1984-08-30 1986-03-25 Meiji Seika Kaisha Ltd ビアラホス生産遺伝子及び耐性遺伝子
CA1238591A (en) 1984-09-27 1988-06-28 Ramachandra N. Rao Recombinant dna cosmid shuttle vectors
EP0463707A1 (de) * 1985-12-17 1992-01-02 Lubrizol Genetics Inc. Herstellung von Polyketid-Antibiotika

Also Published As

Publication number Publication date
HUT40818A (en) 1987-02-27
EP0204549B1 (de) 1994-01-26
US4935340A (en) 1990-06-19
DK259986A (da) 1986-12-08
DE3689572T2 (de) 1994-05-19
EP0204549A2 (de) 1986-12-10
JPS61285991A (ja) 1986-12-16
AU5141690A (en) 1990-08-23
DK259986D0 (da) 1986-06-03
AU5836786A (en) 1986-12-11
AU630930B2 (en) 1992-11-12
HU202924B (en) 1991-04-29
EP0204549A3 (en) 1987-12-02
IL79013A0 (en) 1986-09-30

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