DE3686409D1 - Substratenansatz von mischungen in einem 2-amino-2-methyl-1-propanil enthaltenden puffer fuer alkalin-phosphatase-probe. - Google Patents
Substratenansatz von mischungen in einem 2-amino-2-methyl-1-propanil enthaltenden puffer fuer alkalin-phosphatase-probe.Info
- Publication number
- DE3686409D1 DE3686409D1 DE8686117629T DE3686409T DE3686409D1 DE 3686409 D1 DE3686409 D1 DE 3686409D1 DE 8686117629 T DE8686117629 T DE 8686117629T DE 3686409 T DE3686409 T DE 3686409T DE 3686409 D1 DE3686409 D1 DE 3686409D1
- Authority
- DE
- Germany
- Prior art keywords
- amino
- methyl
- alkaline phosphatase
- propanil
- mixtures
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000203 mixture Substances 0.000 title abstract 3
- 239000000758 substrate Substances 0.000 title abstract 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 title 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 title 1
- 238000009472 formulation Methods 0.000 abstract 2
- QRXMUCSWCMTJGU-UHFFFAOYSA-L (5-bromo-4-chloro-1h-indol-3-yl) phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP([O-])(=O)[O-])=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-L 0.000 abstract 1
- 229940058020 2-amino-2-methyl-1-propanol Drugs 0.000 abstract 1
- 238000008940 Alkaline Phosphatase assay kit Methods 0.000 abstract 1
- CBTVGIZVANVGBH-UHFFFAOYSA-N aminomethyl propanol Chemical compound CC(C)(N)CO CBTVGIZVANVGBH-UHFFFAOYSA-N 0.000 abstract 1
- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/90—Developer
- C12Q2326/92—Nitro blue tetrazolium chloride, i.e. NBT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/50—Indoles
- C12Q2334/52—5-Bromo-4-chloro-3-indolyl, i.e. BCI
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/967—Standards, controls, materials, e.g. validation studies, buffer systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/817,090 US4748115A (en) | 1986-01-08 | 1986-01-08 | Substrate formulation in 2-amino-2-methyl-1-propanol buffer for alkaline phosphatase assays |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3686409D1 true DE3686409D1 (de) | 1992-09-17 |
| DE3686409T2 DE3686409T2 (de) | 1993-02-18 |
Family
ID=25222331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE8686117629T Expired - Fee Related DE3686409T2 (de) | 1986-01-08 | 1986-12-18 | Substratenansatz von mischungen in einem 2-amino-2-methyl-1-propanil enthaltenden puffer fuer alkalin-phosphatase-probe. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4748115A (de) |
| EP (1) | EP0228663B1 (de) |
| JP (1) | JPH0695958B2 (de) |
| AT (1) | ATE79411T1 (de) |
| AU (1) | AU595570B2 (de) |
| CA (1) | CA1293677C (de) |
| DE (1) | DE3686409T2 (de) |
| ES (1) | ES2005545A6 (de) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5443958A (en) * | 1987-08-14 | 1995-08-22 | Director-General, National Institute Of Animal Industry | 2-acrylamine-2-methyl-1-propanesulfonic acid enhancement of alkaline phosphatase label detection |
| US4956302A (en) * | 1987-09-11 | 1990-09-11 | Abbott Laboratories | Lateral flow chromatographic binding assay device |
| US4892817A (en) * | 1987-09-21 | 1990-01-09 | Biogenex Laboratories | Stable phosphatase substrate composition |
| IL85018A0 (en) * | 1988-01-03 | 1988-06-30 | Orgenics Ltd | Stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt,method of making and using same in biological and diagnostic assays |
| US5374524A (en) * | 1988-05-10 | 1994-12-20 | E. I. Du Pont De Nemours And Company | Solution sandwich hybridization, capture and detection of amplified nucleic acids |
| US5137804A (en) * | 1988-05-10 | 1992-08-11 | E. I. Du Pont De Nemours And Company | Assay device and immunoassay |
| US5208143A (en) * | 1988-11-17 | 1993-05-04 | Becton, Dickinson And Company | Immunoassay on a preblocked solid surface |
| US5135847A (en) * | 1988-11-18 | 1992-08-04 | Becton, Dickinson And Company | Substrate composition for alkaline phosphatase and method for assay using same |
| CA2023850A1 (en) * | 1989-09-28 | 1991-03-29 | Randal A. Hoke | Stabilizing reagent for membrane immunoassay |
| KR910014706A (ko) * | 1990-01-10 | 1991-08-31 | 원본미기재 | 세척이 필요없는 개량된 이뮤노어세이(immunoassay)장치 |
| EP0648278B1 (de) * | 1992-07-02 | 1999-12-08 | Roche Diagnostics Corporation | Stabilisierung von tetrazoliumsalzen in einem reagenz |
| ES2145054T3 (es) * | 1992-07-02 | 2000-07-01 | Roche Diagnostics Corp | Un reactivo estabilizado por sales de metal bivalente. |
| DE19639538A1 (de) * | 1996-09-26 | 1998-04-09 | Boehringer Mannheim Gmbh | Enzymatisches Verfahren zum Nachweis der Erhitzungsbehandlung von Milch |
| ES2143934B1 (es) * | 1998-01-23 | 2000-12-16 | Univ Oviedo | Cuantificacion amperometrica y voltamperometrica de azul de indigo para la determinacion de hidrolasas y desarrollo de biosensores. |
| DE19830478A1 (de) * | 1998-07-08 | 2000-01-13 | Friedrich Schiller Uni Jena Bu | Verfahren zum Nachweis von alkalischer Phosphatase und Phosphatasekonjugaten |
| US6099892A (en) * | 1998-10-01 | 2000-08-08 | Pumpkin Ltd. | Protective coating for decorative vegetable material |
| JP4336886B2 (ja) | 2001-09-28 | 2009-09-30 | アークレイ株式会社 | テトラゾリウム化合物の保存方法、それに用いる安定化剤、および前記方法を用いたテトラゾリウム化合物試薬溶液 |
| ATE391793T1 (de) | 2001-10-11 | 2008-04-15 | Arkray Inc | Natriumazid verwendendes messverfahren |
| US11531032B2 (en) * | 2018-01-09 | 2022-12-20 | Idexx Laboratories, Inc. | Methods for measuring analyte and/or protein in biological samples |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4030995A (en) * | 1976-07-13 | 1977-06-21 | Sigma Chemical Company | Alkaline phosphatase isoenzyme determination |
| US4563417A (en) * | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
-
1986
- 1986-01-08 US US06/817,090 patent/US4748115A/en not_active Expired - Lifetime
- 1986-12-18 AT AT86117629T patent/ATE79411T1/de not_active IP Right Cessation
- 1986-12-18 EP EP86117629A patent/EP0228663B1/de not_active Expired
- 1986-12-18 DE DE8686117629T patent/DE3686409T2/de not_active Expired - Fee Related
- 1986-12-24 JP JP61306696A patent/JPH0695958B2/ja not_active Expired - Lifetime
-
1987
- 1987-01-05 AU AU67133/87A patent/AU595570B2/en not_active Ceased
- 1987-01-05 ES ES8700017A patent/ES2005545A6/es not_active Expired
- 1987-01-07 CA CA000526888A patent/CA1293677C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0695958B2 (ja) | 1994-11-30 |
| AU595570B2 (en) | 1990-04-05 |
| ATE79411T1 (de) | 1992-08-15 |
| CA1293677C (en) | 1991-12-31 |
| EP0228663A2 (de) | 1987-07-15 |
| EP0228663B1 (de) | 1992-08-12 |
| ES2005545A6 (es) | 1989-03-16 |
| EP0228663A3 (en) | 1988-06-01 |
| DE3686409T2 (de) | 1993-02-18 |
| AU6713387A (en) | 1987-07-09 |
| JPS62166899A (ja) | 1987-07-23 |
| US4748115A (en) | 1988-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |