DE3641830A1 - Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich - Google Patents

Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich

Info

Publication number
DE3641830A1
DE3641830A1 DE19863641830 DE3641830A DE3641830A1 DE 3641830 A1 DE3641830 A1 DE 3641830A1 DE 19863641830 DE19863641830 DE 19863641830 DE 3641830 A DE3641830 A DE 3641830A DE 3641830 A1 DE3641830 A1 DE 3641830A1
Authority
DE
Germany
Prior art keywords
concentration
antibody
detection
test
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
DE19863641830
Other languages
German (de)
English (en)
Other versions
DE3641830C2 (enExample
Inventor
Dieter Dipl Biol Bryniok
Iris Dr Trick
Walter Dr Troesch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer-Gesellschaft zur Förderung der Angewandten Forschung eV
Original Assignee
Fraunhofer-Gesellschaft zur Förderung der Angewandten Forschung eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer-Gesellschaft zur Förderung der Angewandten Forschung eV filed Critical Fraunhofer-Gesellschaft zur Förderung der Angewandten Forschung eV
Priority to DE19863641830 priority Critical patent/DE3641830A1/de
Priority to AT87907935T priority patent/ATE89034T1/de
Priority to EP87907935A priority patent/EP0335884B1/de
Priority to PCT/DE1987/000583 priority patent/WO1988004328A1/de
Publication of DE3641830A1 publication Critical patent/DE3641830A1/de
Application granted granted Critical
Publication of DE3641830C2 publication Critical patent/DE3641830C2/de
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/10—Benzidines
    • C12Q2326/12—3,3',5,5'-Tetramethylbenzidine, i.e. TMB

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE19863641830 1986-12-08 1986-12-08 Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich Granted DE3641830A1 (de)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE19863641830 DE3641830A1 (de) 1986-12-08 1986-12-08 Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich
AT87907935T ATE89034T1 (de) 1986-12-08 1987-12-08 Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich.
EP87907935A EP0335884B1 (de) 1986-12-08 1987-12-08 Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich
PCT/DE1987/000583 WO1988004328A1 (fr) 1986-12-08 1987-12-08 Test immunologique a enzymes de depistage par changement de couleurs dans le spectre visible

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19863641830 DE3641830A1 (de) 1986-12-08 1986-12-08 Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich

Publications (2)

Publication Number Publication Date
DE3641830A1 true DE3641830A1 (de) 1988-06-09
DE3641830C2 DE3641830C2 (enExample) 1989-02-16

Family

ID=6315672

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19863641830 Granted DE3641830A1 (de) 1986-12-08 1986-12-08 Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich

Country Status (3)

Country Link
EP (1) EP0335884B1 (enExample)
DE (1) DE3641830A1 (enExample)
WO (1) WO1988004328A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4013004C2 (de) * 1990-04-24 1993-10-21 Elvira Schecklies Verfahren zur quantitativen Bestimmung von niedermolekularen organischen Substanzen
US6958039B2 (en) 2003-05-02 2005-10-25 Oculir, Inc. Method and instruments for non-invasive analyte measurement
US6975892B2 (en) 2003-10-21 2005-12-13 Oculir, Inc. Methods for non-invasive analyte measurement from the conjunctiva
US6968222B2 (en) 2003-05-02 2005-11-22 Oculir, Inc. Methods and device for non-invasive analyte measurement
CN116735511B (zh) * 2023-06-29 2024-05-31 徐詹程 一种酶促反应分析酶浓度拟合标准曲线的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4503143A (en) * 1982-08-20 1985-03-05 Btc Diagnostics Limited Partnership Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2906732C2 (de) * 1979-02-21 1981-10-01 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und Reagenz zur Bestimmung der Peroxidase
US4596770A (en) * 1984-06-01 1986-06-24 Travenol-Genetech Diagnostics Assay of peroxidase enzyme activity
GB8505899D0 (en) * 1985-03-07 1985-04-11 Boots Celltech Diagnostics Assay reagents
DE3541978A1 (de) * 1985-11-28 1987-06-04 Behringwerke Ag Mittel zur bestimmung von peroxidase-aktivitaet, verfahren zur herstellung und seine verwendung

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4503143A (en) * 1982-08-20 1985-03-05 Btc Diagnostics Limited Partnership Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
G. Müller, Klin.Bichochem. u. Laboratoriumsdiagn.,Stuttgart u. New York 1977, S. 59/60 *

Also Published As

Publication number Publication date
EP0335884A1 (de) 1989-10-11
EP0335884B1 (de) 1993-05-05
DE3641830C2 (enExample) 1989-02-16
WO1988004328A1 (fr) 1988-06-16

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Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
D2 Grant after examination
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee