DE3539977C2 - - Google Patents
Info
- Publication number
- DE3539977C2 DE3539977C2 DE3539977A DE3539977A DE3539977C2 DE 3539977 C2 DE3539977 C2 DE 3539977C2 DE 3539977 A DE3539977 A DE 3539977A DE 3539977 A DE3539977 A DE 3539977A DE 3539977 C2 DE3539977 C2 DE 3539977C2
- Authority
- DE
- Germany
- Prior art keywords
- light
- spectroscope
- sample
- fluorescence
- excitation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000005284 excitation Effects 0.000 claims description 21
- 238000012544 monitoring process Methods 0.000 claims description 19
- 230000003595 spectral effect Effects 0.000 claims description 4
- 238000000354 decomposition reaction Methods 0.000 claims 2
- 238000012937 correction Methods 0.000 description 11
- 238000001228 spectrum Methods 0.000 description 11
- 238000005259 measurement Methods 0.000 description 9
- 238000001514 detection method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000005855 radiation Effects 0.000 description 4
- 229910052724 xenon Inorganic materials 0.000 description 4
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 4
- 238000001917 fluorescence detection Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- 229940043267 rhodamine b Drugs 0.000 description 2
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4406—Fluorescence spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/10—Photometry, e.g. photographic exposure meter by comparison with reference light or electric value provisionally void
- G01J1/16—Photometry, e.g. photographic exposure meter by comparison with reference light or electric value provisionally void using electric radiation detectors
- G01J1/1626—Arrangements with two photodetectors, the signals of which are compared
- G01J2001/1636—Arrangements with two photodetectors, the signals of which are compared one detector directly monitoring the source, e.g. also impulse time controlling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/04—Slit arrangements slit adjustment
- G01J2003/042—Slit wheel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J2003/2866—Markers; Calibrating of scan
- G01J2003/2873—Storing reference spectrum
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Physics & Mathematics (AREA)
- Spectrometry And Color Measurement (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP59238987A JPS61116627A (ja) | 1984-11-12 | 1984-11-12 | 分光蛍光光度計 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3539977A1 DE3539977A1 (de) | 1986-05-22 |
DE3539977C2 true DE3539977C2 (en, 2012) | 1989-10-05 |
Family
ID=17038237
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE19853539977 Granted DE3539977A1 (de) | 1984-11-12 | 1985-11-11 | Spektrofluorophotometer |
Country Status (3)
Country | Link |
---|---|
US (1) | US4779982A (en, 2012) |
JP (1) | JPS61116627A (en, 2012) |
DE (1) | DE3539977A1 (en, 2012) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6317423U (en, 2012) * | 1986-07-17 | 1988-02-05 | ||
DE3915421C2 (de) * | 1989-05-11 | 1995-03-02 | Bayer Ag | Vorrichtung zur Messung der Fluoreszenzanregung biologischer Zellen bei zwei verschiedenen Wellenlängen |
US6332573B1 (en) | 1998-11-10 | 2001-12-25 | Ncr Corporation | Produce data collector and produce recognition system |
US6155489A (en) * | 1998-11-10 | 2000-12-05 | Ncr Corporation | Item checkout device including a bar code data collector and a produce data collector |
US6431446B1 (en) | 1999-07-28 | 2002-08-13 | Ncr Corporation | Produce recognition system and method |
DE10317881A1 (de) | 2003-04-17 | 2004-11-11 | Consortium für elektrochemische Industrie GmbH | Isocyanatfreie schäumbare Mischungen mit verbessertem Brandverhalten |
US7151270B2 (en) * | 2003-05-02 | 2006-12-19 | Leica Microsystems Cms Gmbh | Method for classifying object image regions of an object to be detected using a scanning microscope |
US8760645B2 (en) | 2011-05-24 | 2014-06-24 | Idexx Laboratories Inc. | Method of normalizing a fluorescence analyzer |
CA3221112A1 (en) | 2021-06-04 | 2022-12-08 | Garland Christian Misener | Method for callibrating sensitivity of a photometer |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS4831989A (en, 2012) * | 1971-08-27 | 1973-04-26 | ||
US3832555A (en) * | 1973-04-10 | 1974-08-27 | Hitachi Ltd | Fluorescence spectrophotometer |
US3967113A (en) * | 1974-12-05 | 1976-06-29 | Baxter Laboratories, Inc. | Wavelength-corrected spectrofluorometer |
DE2535398A1 (de) * | 1975-08-08 | 1977-02-24 | Applied Photophysics Ltd | Spektralfluoreszenzmesser |
US4022529A (en) * | 1975-12-11 | 1977-05-10 | White John U | Feature extraction system for extracting a predetermined feature from a signal |
US4203670A (en) * | 1977-04-21 | 1980-05-20 | Bromberg Nathan S | System and method of fluorescence polarimetry |
US4198567A (en) * | 1977-10-25 | 1980-04-15 | Peter Eneroth | Method and apparatus for discrimination between scattered excitation radiation and low level fast decaying fluorescent radiation |
JPS5979819A (ja) * | 1982-10-29 | 1984-05-09 | Shimadzu Corp | 分光器におけるスリツト機構 |
JPS5981520A (ja) * | 1982-10-30 | 1984-05-11 | Shimadzu Corp | 分光器スリツト機構 |
US4647199A (en) * | 1984-09-14 | 1987-03-03 | The Perkin-Elmer Corporation | Automatic shutter system |
US4622468A (en) * | 1985-07-15 | 1986-11-11 | Sequoia-Turner Corporation | Fluorescence intensity compensation method and device |
-
1984
- 1984-11-12 JP JP59238987A patent/JPS61116627A/ja active Pending
-
1985
- 1985-11-11 DE DE19853539977 patent/DE3539977A1/de active Granted
- 1985-11-12 US US06/796,808 patent/US4779982A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US4779982A (en) | 1988-10-25 |
JPS61116627A (ja) | 1986-06-04 |
DE3539977A1 (de) | 1986-05-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP0098423B1 (de) | Gitterspektrometer | |
DE3833602C2 (en, 2012) | ||
DE2642170C2 (de) | Spektrophotometer | |
DE2902776C2 (en, 2012) | ||
DE2935716C2 (en, 2012) | ||
DE2727976C3 (de) | Vorrichtung zur Messung der Konzentration mindestens einer Komponente eines Gasgemisches und Verfahren zum Eichen derselben | |
DE2364069C3 (de) | Spektralphotometer | |
DE3429541C2 (de) | Verfahren zur gleichzeitigen Bestimmung der Wellenlänge und der Strahlungsleistung einer monochromatischen Lichtquelle | |
DE19912500A1 (de) | Verfahren und Vorrichtung zum Bestimmen von Eigenschaften einer laufenden Materialbahn | |
DE2147142C3 (de) | Photometer für die quantitative Analyse von in einer Lösung enthaltenen Elementen | |
DE3724852C2 (de) | Absorptionsphotometer | |
DE3937141C2 (en, 2012) | ||
DE2436110B2 (de) | Vorrichtung zur Feststellung von Herstellungsfehlern in einer bewegten Materialbahn | |
EP0098429A2 (de) | Monochromator | |
DE2747387A1 (de) | Spektralphotometer | |
DE19601873C2 (de) | Gasanalysator | |
DE3643764A1 (de) | Verfahren zur selektiven fuellstoffmessung an laufenden materialbahnen, insbesondere papierbahnen | |
EP0096247B1 (de) | Vorrichtung zum betrieblichen Erfassen eines Masses für die Feuchtmittelmenge auf der rotierenden Druckplatte in Offset-Druckmaschinen | |
DE69204975T2 (de) | Verfahren zur Ermittlung der Information über den Aufbau einer mehrschichtigen Bahn. | |
DE3539977C2 (en, 2012) | ||
DE3038908C2 (en, 2012) | ||
CH382463A (de) | Nach dem Kompensationsprinzip arbeitende Strahlenvergleichsvorrichtung | |
DE3914135C2 (en, 2012) | ||
DE3541165A1 (de) | Vorrichtung zur kontinuierlichen bestimmung von konzentrationsaenderungen in stoffgemischen | |
DE2207298B2 (de) | Verfahren und vorrichtung fuer die atomabsorptionsspektralanalyse |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
OP8 | Request for examination as to paragraph 44 patent law | ||
D2 | Grant after examination | ||
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |