DE3526829A1 - Verfahren und reagenzeinheit zum bestimmen von alpha-amylase - Google Patents
Verfahren und reagenzeinheit zum bestimmen von alpha-amylaseInfo
- Publication number
- DE3526829A1 DE3526829A1 DE19853526829 DE3526829A DE3526829A1 DE 3526829 A1 DE3526829 A1 DE 3526829A1 DE 19853526829 DE19853526829 DE 19853526829 DE 3526829 A DE3526829 A DE 3526829A DE 3526829 A1 DE3526829 A1 DE 3526829A1
- Authority
- DE
- Germany
- Prior art keywords
- alpha
- amylase
- bond
- glucose
- glucosidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 102000004139 alpha-Amylases Human genes 0.000 title claims abstract description 38
- 108090000637 alpha-Amylases Proteins 0.000 title claims abstract description 38
- 229940024171 alpha-amylase Drugs 0.000 title claims abstract description 37
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims description 37
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims abstract description 46
- 239000000758 substrate Substances 0.000 claims abstract description 35
- 102100024295 Maltase-glucoamylase Human genes 0.000 claims abstract description 22
- 230000000903 blocking effect Effects 0.000 claims abstract description 22
- 229940088598 enzyme Drugs 0.000 claims abstract description 22
- 108010028144 alpha-Glucosidases Proteins 0.000 claims abstract description 21
- 102000006995 beta-Glucosidase Human genes 0.000 claims abstract description 15
- 108010047754 beta-Glucosidase Proteins 0.000 claims abstract description 15
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 13
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 13
- 125000001424 substituent group Chemical group 0.000 claims abstract description 13
- 230000008859 change Effects 0.000 claims abstract description 11
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 9
- 230000007017 scission Effects 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 3
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 17
- 102100022624 Glucoamylase Human genes 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 10
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical group OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 claims description 8
- HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 claims description 6
- 238000002372 labelling Methods 0.000 claims description 4
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 claims description 3
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims description 2
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims description 2
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims description 2
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims description 2
- 239000003550 marker Substances 0.000 claims 3
- CFNMUZCFSDMZPQ-GHXNOFRVSA-N 7-[(z)-3-methyl-4-(4-methyl-5-oxo-2h-furan-2-yl)but-2-enoxy]chromen-2-one Chemical compound C=1C=C2C=CC(=O)OC2=CC=1OC/C=C(/C)CC1OC(=O)C(C)=C1 CFNMUZCFSDMZPQ-GHXNOFRVSA-N 0.000 claims 2
- 101710084890 Beta-glucosidase A Proteins 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 description 21
- 239000008103 glucose Substances 0.000 description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- -1 hydroxyL group Chemical group 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 7
- 230000003287 optical effect Effects 0.000 description 7
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000005259 measurement Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 125000000217 alkyl group Chemical group 0.000 description 3
- 125000003118 aryl group Chemical group 0.000 description 3
- 125000000649 benzylidene group Chemical group [H]C(=[*])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 230000008020 evaporation Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 102000013142 Amylases Human genes 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 150000001241 acetals Chemical class 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 125000003710 aryl alkyl group Chemical group 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- 125000000636 p-nitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000001665 trituration Methods 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- FEWDXGMBVQULLN-UHFFFAOYSA-N 1-hydroxy-2-phenyl-1,5,6,7-tetrahydro-4H-benzimidazol-4-one Chemical compound ON1C=2CCCC(=O)C=2N=C1C1=CC=CC=C1 FEWDXGMBVQULLN-UHFFFAOYSA-N 0.000 description 1
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 102000005548 Hexokinase Human genes 0.000 description 1
- 108700040460 Hexokinases Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 239000007990 PIPES buffer Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 208000016222 Pancreatic disease Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000010933 acylation Effects 0.000 description 1
- 238000005917 acylation reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- BNABBHGYYMZMOA-AHIHXIOASA-N alpha-maltoheptaose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@H](O[C@H](O[C@@H]3[C@H](O[C@H](O[C@@H]4[C@H](O[C@H](O[C@@H]5[C@H](O[C@H](O[C@@H]6[C@H](O[C@H](O)[C@H](O)[C@H]6O)CO)[C@H](O)[C@H]5O)CO)[C@H](O)[C@H]4O)CO)[C@H](O)[C@H]3O)CO)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O BNABBHGYYMZMOA-AHIHXIOASA-N 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- ULKGULQGPBMIJU-UHFFFAOYSA-N benzene;hydron;bromide Chemical compound Br.C1=CC=CC=C1 ULKGULQGPBMIJU-UHFFFAOYSA-N 0.000 description 1
- 125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 description 1
- 229960004217 benzyl alcohol Drugs 0.000 description 1
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- 239000013060 biological fluid Substances 0.000 description 1
- 150000001733 carboxylic acid esters Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 229940097362 cyclodextrins Drugs 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 125000004170 methylsulfonyl group Chemical group [H]C([H])([H])S(*)(=O)=O 0.000 description 1
- 125000001736 nosyl group Chemical group S(=O)(=O)(C1=CC=C([N+](=O)[O-])C=C1)* 0.000 description 1
- 239000012044 organic layer Substances 0.000 description 1
- 125000001181 organosilyl group Chemical group [SiH3]* 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 238000012746 preparative thin layer chromatography Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 150000003871 sulfonates Chemical class 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 125000005147 toluenesulfonyl group Chemical group C=1(C(=CC=CC1)S(=O)(=O)*)C 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/634,873 US4649108A (en) | 1984-07-26 | 1984-07-26 | Alpha amylase assay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3526829A1 true DE3526829A1 (de) | 1986-02-06 |
Family
ID=24545509
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19853526829 Ceased DE3526829A1 (de) | 1984-07-26 | 1985-07-26 | Verfahren und reagenzeinheit zum bestimmen von alpha-amylase |
| DE8585305355T Expired DE3569572D1 (en) | 1984-07-26 | 1985-07-26 | An alpha-amylase assay method and reagent kit |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE8585305355T Expired DE3569572D1 (en) | 1984-07-26 | 1985-07-26 | An alpha-amylase assay method and reagent kit |
Country Status (8)
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3328616A1 (de) * | 1983-08-08 | 1985-02-28 | Boehringer Mannheim Gmbh, 6800 Mannheim | Oligoglucosidderivate |
| US4794078A (en) * | 1984-07-26 | 1988-12-27 | Genzyme Corporation | Alpha amylase assay |
| DE3508384A1 (de) * | 1985-03-08 | 1986-11-06 | Boehringer Mannheim Gmbh, 6800 Mannheim | Hemmung humaner speichel- und pankreasamylase durch monoklonale antikoerper |
| DE3537877A1 (de) * | 1985-10-24 | 1987-04-30 | Geiger Reinhard | Luciferin-derivate und immunoassays unter einsatz derartiger luciferin-derivate |
| DE3621271A1 (de) * | 1986-06-25 | 1988-01-07 | Boehringer Mannheim Gmbh | Verfahren und reagenz zur bestimmung von alpha-amylase |
| ES2060584T3 (es) * | 1986-07-11 | 1994-12-01 | Wako Pure Chem Ind Ltd | Ensayo en cuanto a alfa-amilasa que utiliza un oligosacarido modificado y procedimiento para producir tal oligosacarido modificado. |
| US5192666A (en) * | 1986-07-11 | 1993-03-09 | Wako Pure Chemical Industries, Ltd. | α-amylase assay using modified oligosaccharide and process for producing said modified oligosaccharide |
| JPH07112440B2 (ja) * | 1986-08-01 | 1995-12-06 | 和光純薬工業株式会社 | α−アミラ−ゼアイソザイムの分別測定法 |
| EP0259521A1 (en) * | 1986-09-10 | 1988-03-16 | Akzo N.V. | Test reagent for amylase determination |
| US4810636A (en) * | 1986-12-09 | 1989-03-07 | Miles Inc. | Chromogenic acridinone enzyme substrates |
| EP0371994B1 (en) * | 1987-07-17 | 1995-07-05 | Modrovich, Ivan Endre | Stable, single-liquid alpha-amylase reagent |
| JP2592260B2 (ja) * | 1987-07-30 | 1997-03-19 | オリエンタル酵母工業株式会社 | α−アミラーゼアイソザイムの分別定量法 |
| GB8722278D0 (en) * | 1987-09-22 | 1987-10-28 | Genetics Int Inc | Determination of amylase |
| JPH0631293B2 (ja) * | 1987-12-11 | 1994-04-27 | 東洋紡績株式会社 | マルトオリゴ糖誘導体およびアミラーゼ活性測定用試薬 |
| DE3743908A1 (de) * | 1987-12-23 | 1989-07-06 | Boehringer Mannheim Gmbh | Neue oligoglucoside |
| DE3929355A1 (de) * | 1989-09-04 | 1991-03-07 | Boehringer Mannheim Gmbh | Verfahren zur spezifischen bestimmung von pankreas-(alpha)-amylase |
| CA2039564A1 (en) * | 1990-04-10 | 1991-10-11 | James R. Rasmussen | Oligosaccharide compounds and preparation thereof |
| US5300634A (en) * | 1991-05-07 | 1994-04-05 | Wako Pure Chemical Industries, Ltd. | Process for producing maltooligosaccharide derivative |
| GB9112989D0 (en) * | 1991-06-17 | 1991-08-07 | Genzyme Uk Ltd | Preparation of oligosaccharide derivatives |
| JP3075377B2 (ja) * | 1991-11-06 | 2000-08-14 | 東洋紡績株式会社 | α−アミラーゼ活性の測定法およびα−アミラーゼ活性測定用試薬 |
| CA2089228A1 (en) * | 1992-02-14 | 1993-08-15 | Akira Hasegawa | Oligosaccharide derivatives suitable for .alpha.-amylase determination |
| JPH05294984A (ja) * | 1992-04-15 | 1993-11-09 | Wako Pure Chem Ind Ltd | マルトオリゴ糖誘導体の新規な製造方法 |
| US5384245A (en) * | 1992-05-04 | 1995-01-24 | Ivan E. Modrovich | Stable, single-liquid alpha-amylase reagent |
| GB9525403D0 (en) * | 1995-12-13 | 1996-02-14 | Univ Sunderland | Method for monitoring enzymes |
| US7299805B2 (en) | 2002-06-07 | 2007-11-27 | Marctec, Llc | Scaffold and method for implanting cells |
| US20080050451A1 (en) * | 2006-06-16 | 2008-02-28 | Mabry Helen C | Methods for assessing dehydration and shock, assays and kits for the methods |
| DE602008003242D1 (de) * | 2007-01-17 | 2010-12-16 | Fujifilm Corp | Verfahren zur Messung tierischer Alpha-Amylase |
| CA2787021A1 (en) * | 2009-02-05 | 2010-08-12 | Hydradx, Inc. | Diagnostic device and method |
| EP2719758A1 (en) * | 2012-10-09 | 2014-04-16 | Megazyme IP Limited | Cellulase assay |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4102747A (en) * | 1977-07-28 | 1978-07-25 | American Hospital Supply Corporation | Amylase determination |
| EP0104047A2 (en) * | 1982-09-16 | 1984-03-28 | Wako Pure Chemical Industries, Ltd. | Modified oligosaccharides used as substrate for measuring alpha-amylase activity |
| DE3328616A1 (de) * | 1983-08-08 | 1985-02-28 | Boehringer Mannheim Gmbh, 6800 Mannheim | Oligoglucosidderivate |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3876375A (en) * | 1971-11-22 | 1975-04-08 | Jonas Maurukas | Biological composition for use as a reference control in diagnostic analysis |
| GB1381380A (en) * | 1972-09-06 | 1975-01-22 | Warner Lambert Co | Water-soluble dyed substrate for amylase assay |
| US4310625A (en) * | 1976-03-17 | 1982-01-12 | Modrovich Ivan Endre | Stabilized liquid enzyme compositions for diagnostic determinations |
| US4153511A (en) * | 1976-03-17 | 1979-05-08 | Modrovich Ivan Endre | Stabilized liquid coenzyme compositions |
| CA1068586A (en) * | 1976-07-01 | 1979-12-25 | Abbott Laboratories | .alpha.-AMYLASE ASSAY UTILIZING MODIFIED STARCH AS THE SUBSTRATE |
| US4145527A (en) * | 1976-07-13 | 1979-03-20 | E. I. Du Pont De Nemours And Company | Nitro aromatic glycosides |
| NL179399C (nl) * | 1976-07-13 | 1986-09-01 | Du Pont | Werkwijze voor de bepaling van het amylase-gehalte van een monster en reagens-proefpakket daarvoor. |
| US4147860A (en) * | 1976-07-13 | 1979-04-03 | E. I. Du Pont De Nemours And Company | Process for preparing nitroaromatic glycosides |
| US4271264A (en) * | 1976-09-13 | 1981-06-02 | Modrovich Ivan Endre | Stabilized liquid enzyme and coenzyme compositions |
| US4277562A (en) * | 1976-09-13 | 1981-07-07 | Modrovich Ivan Endre | Stabilized liquid enzyme and coenzyme compositions |
| AT362526B (de) * | 1977-09-13 | 1981-05-25 | Boehringer Mannheim Gmbh | Verfahren und reagens zur bestimmung von alpha- -amylase |
| US4233403A (en) * | 1978-02-27 | 1980-11-11 | E. I. Du Pont De Nemours And Company | Amylase assay |
| US4250254A (en) * | 1978-09-11 | 1981-02-10 | Modrovich Ivan Endre | Stabilized liquid enzyme and coenzyme compositions |
| US4282316A (en) * | 1979-09-11 | 1981-08-04 | Modrovich Ivan Endre | Stabilized enzymic solutions for determining urea |
| US4550077A (en) * | 1980-05-07 | 1985-10-29 | Electro-Nucleonics, Inc. | β-Amylase determination |
| JPS5768798A (en) * | 1980-10-14 | 1982-04-27 | Toyo Jozo Co Ltd | Novel measurement of amylase activity |
| JPS5931699A (ja) * | 1982-08-17 | 1984-02-20 | Wako Pure Chem Ind Ltd | α−アミラ−ゼ活性の測定法 |
| JPS5951800A (ja) * | 1982-09-16 | 1984-03-26 | Wako Pure Chem Ind Ltd | α−アミラ−ゼの活性測定方法 |
| DE3323245A1 (de) * | 1983-06-28 | 1985-01-10 | Merck Patent Gmbh, 6100 Darmstadt | Verfahren und reagenz zur bestimmung von (alpha)-amylase |
| JPS60237998A (ja) * | 1983-12-22 | 1985-11-26 | Toyobo Co Ltd | α−アミラ−ゼ活性測定法 |
-
1984
- 1984-07-26 US US06/634,873 patent/US4649108A/en not_active Expired - Lifetime
-
1985
- 1985-07-24 IL IL75912A patent/IL75912A/xx not_active IP Right Cessation
- 1985-07-24 CA CA000487404A patent/CA1260369A/en not_active Expired
- 1985-07-26 GB GB08518894A patent/GB2166545B/en not_active Expired
- 1985-07-26 JP JP60165625A patent/JPS6163299A/ja active Granted
- 1985-07-26 DE DE19853526829 patent/DE3526829A1/de not_active Ceased
- 1985-07-26 AT AT85305355T patent/ATE42342T1/de not_active IP Right Cessation
- 1985-07-26 DE DE8585305355T patent/DE3569572D1/de not_active Expired
- 1985-07-26 EP EP85305355A patent/EP0171960B1/en not_active Expired
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4102747A (en) * | 1977-07-28 | 1978-07-25 | American Hospital Supply Corporation | Amylase determination |
| GB1571265A (en) * | 1977-07-28 | 1980-07-09 | American Hospital Supply Corp | Amylase determination |
| EP0104047A2 (en) * | 1982-09-16 | 1984-03-28 | Wako Pure Chemical Industries, Ltd. | Modified oligosaccharides used as substrate for measuring alpha-amylase activity |
| DE3328616A1 (de) * | 1983-08-08 | 1985-02-28 | Boehringer Mannheim Gmbh, 6800 Mannheim | Oligoglucosidderivate |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE42342T1 (de) | 1989-05-15 |
| DE3569572D1 (en) | 1989-05-24 |
| IL75912A0 (en) | 1985-12-31 |
| US4649108A (en) | 1987-03-10 |
| EP0171960A1 (en) | 1986-02-19 |
| CA1260369A (en) | 1989-09-26 |
| IL75912A (en) | 1989-07-31 |
| GB8518894D0 (en) | 1985-09-04 |
| EP0171960B1 (en) | 1989-04-19 |
| GB2166545A (en) | 1986-05-08 |
| JPS6365314B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1988-12-15 |
| JPS6163299A (ja) | 1986-04-01 |
| GB2166545B (en) | 1988-05-05 |
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