DE3375159D1 - Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations - Google Patents

Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations

Info

Publication number
DE3375159D1
DE3375159D1 DE8383108453T DE3375159T DE3375159D1 DE 3375159 D1 DE3375159 D1 DE 3375159D1 DE 8383108453 T DE8383108453 T DE 8383108453T DE 3375159 T DE3375159 T DE 3375159T DE 3375159 D1 DE3375159 D1 DE 3375159D1
Authority
DE
Germany
Prior art keywords
accelerator
substrates
substrate
percent
determinations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE8383108453T
Other languages
English (en)
Inventor
Robert L Stout
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ENZYME TECH CO
Original Assignee
ENZYME TECH CO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ENZYME TECH CO filed Critical ENZYME TECH CO
Application granted granted Critical
Publication of DE3375159D1 publication Critical patent/DE3375159D1/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/30—2,2'-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid), i.e. ABTS
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/805—Test papers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/826—Additives, e.g. buffers, diluents, preservatives
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/904—Oxidation - reduction indicators
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y10T436/206664—Ozone or peroxide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DE8383108453T 1982-09-22 1983-08-27 Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations Expired DE3375159D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/421,263 US4521511A (en) 1982-09-22 1982-09-22 Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations

Publications (1)

Publication Number Publication Date
DE3375159D1 true DE3375159D1 (en) 1988-02-11

Family

ID=23669837

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8383108453T Expired DE3375159D1 (en) 1982-09-22 1983-08-27 Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations

Country Status (7)

Country Link
US (1) US4521511A (de)
EP (1) EP0103784B1 (de)
JP (1) JPS5978700A (de)
AT (1) ATE31734T1 (de)
CA (1) CA1201369A (de)
DE (1) DE3375159D1 (de)
FI (1) FI833311A (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0116454B1 (de) * 1983-02-11 1987-04-29 National Research Development Corporation Erhöhte lumineszierende oder luminometrische Prüfung
GB8303820D0 (en) * 1983-02-11 1983-03-16 Secr Social Service Brit Enhanced luminescent/luminometric immunoassay
JPS59230161A (ja) * 1983-06-13 1984-12-24 Wako Pure Chem Ind Ltd 還元性物質の分解方法及び分解用試薬
DE3413693A1 (de) * 1984-04-11 1985-10-17 Boehringer Mannheim Gmbh, 6800 Mannheim Fluorierte anilinderivate und ihre verwendung
JPS6178385A (ja) * 1984-09-25 1986-04-21 Toyobo Co Ltd 安定なペルオキシダ−ゼ組成物
EP0196743A3 (de) * 1985-01-31 1988-10-19 Savyon Diagnostics Ltd. Chromogenstoffe und Superoxide enthaltende stabile chemische Zusammensetzungen und Verfahren zu deren Gewinnung
IL74205A (en) * 1985-01-31 1990-01-18 Savyon Diagnostics Ltd Method for carrying out enzyme assays utilizing stable chemical compositions containing hydrogen peroxide and a chromogen
DE3509238A1 (de) * 1985-03-14 1986-09-18 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisierung der aktivitaet von peroxidase in loesung
CA1307480C (en) 1985-07-10 1992-09-15 Nanibhushan Dattagupta Prolonged chemiluminescence
US4794073A (en) * 1985-07-10 1988-12-27 Molecular Diagnostics, Inc. Detection of nucleic acid hybrids by prolonged chemiluminescence
US4853327A (en) * 1985-07-10 1989-08-01 Molecular Diagnostics, Inc. Enhanced phthalazinedione chemiluminescence
US4828983A (en) * 1986-07-10 1989-05-09 Eastman Kodak Company Use of phenols and anilines to increase the rate of peroxidase catalyzed oxidation of leuco dyes
US4962024A (en) * 1988-08-11 1990-10-09 Becton, Dickinson And Company Signal enhancement in assay for an enzyme
US5017471A (en) * 1988-08-26 1991-05-21 Epitope, Inc. Reagent for peroxidase detection
US5232858A (en) * 1989-04-25 1993-08-03 Avl Medical Instruments Ag Method for quantitative determination of at least one chemical parameter of a sample medium
WO1991005063A1 (en) * 1989-10-05 1991-04-18 Exoxemis, Inc. Haloperoxidase acid optimum chemiluminescence assay system
US5108899A (en) * 1989-10-31 1992-04-28 Exoxemis, Inc. Chemiluminescence assay of in vivo inflammation
DE4037764A1 (de) * 1990-11-28 1992-06-04 Behringwerke Ag Waschloesung fuer festphasen-immunometrische verfahren, die stabilisatoren fuer das markierungssystem enthaelt und ihre verwendung
US5482832A (en) * 1992-07-08 1996-01-09 Akzo Nobel N.V. Hybridization assays using enzyme-linked probes
US5279940A (en) * 1992-08-03 1994-01-18 Eastman Kodak Company Chemiluminescent composition containing cationic surfactants or polymers and 4'-hydroxyacetanilide, test kits and their use in analytical methods
WO1999001768A1 (en) * 1997-07-04 1999-01-14 Nycomed Amersham Plc Peroxidase-catalysed fluorescence
AU9438498A (en) * 1997-08-28 1999-03-22 Societe Des Produits Nestle S.A. Test method for peroxidase
US6372937B1 (en) 1998-11-09 2002-04-16 Mark Norman Bobrow Enhanced catalyzed reporter deposition
US6617125B2 (en) 2001-06-29 2003-09-09 Perkinelmer Life Sciences, Inc. Compositions for enhanced catalyzed reporter deposition
US6897036B2 (en) * 2002-06-06 2005-05-24 Lumigen, Inc. Fluorescent detection of peroxidase enzymes
US20090029401A1 (en) * 2007-07-25 2009-01-29 Richmond Chemical Corporation Method for detecting biofuel producing microbes
US9017950B2 (en) * 2011-03-18 2015-04-28 Luminescent MD, LLC Method for enhancing enzyme assays
US11237170B2 (en) 2018-12-04 2022-02-01 Aat Bioquest, Inc. Styryl phenols, derivatives and their use in methods of analyte detection

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US29498A (en) * 1860-08-07 Bedstead
US3290228A (en) * 1964-07-29 1966-12-06 Miles Lab Test composition and device for detecting glucose
DE1598133B2 (de) * 1966-09-01 1974-05-22 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren und diagnostisches Mittel zur Bestimmung von Hydroperoxiden bzw. peroxidatisch wirksamen Substanzen
USRE29498E (en) 1972-05-12 1977-12-20 Istituto Sieroterapico e Vaccinogeno Toscano "SCLAVO", S.p.A. Process for the enzymatic determination of glucose with a glucose-oxydazed/peroxidazed enzyme system
NL7801413A (nl) * 1977-03-09 1978-09-12 Hoffmann La Roche Peroxydasebepaling.
JPS561896A (en) * 1979-06-19 1981-01-10 Eiken Kagaku Kk Determination of hydrogen peroxide and reagent for it
US4340392A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340393A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4290773A (en) * 1979-11-13 1981-09-22 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4340394A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
US4278439A (en) * 1979-12-17 1981-07-14 Miles Laboratories, Inc. Sensitizers for peroxidative activity tests
US4251222A (en) * 1979-12-17 1981-02-17 Miles Laboratories, Inc. Sensitizers for peroxidative activity tests
JPS5754358A (ja) * 1980-09-18 1982-03-31 Nec Corp Shusekikairosochi
JPS5783287A (en) * 1980-11-14 1982-05-25 Kyowa Hakko Kogyo Co Ltd Elimination of hydrogen peroxide
JPS57174099A (en) * 1981-04-17 1982-10-26 Fuji Photo Film Co Ltd Color indicator composition for detecting hydrogen peroxide and quantitative analytical film having reagent layer containing the same

Also Published As

Publication number Publication date
US4521511A (en) 1985-06-04
EP0103784A3 (en) 1984-06-06
FI833311A0 (fi) 1983-09-16
EP0103784B1 (de) 1988-01-07
CA1201369A (en) 1986-03-04
EP0103784A2 (de) 1984-03-28
FI833311A (fi) 1984-03-23
ATE31734T1 (de) 1988-01-15
JPS5978700A (ja) 1984-05-07

Similar Documents

Publication Publication Date Title
DE3375159D1 (en) Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations
MX9702041A (es) Intensificadores tales como la acetosiringona.
JPS55500889A (de)
AU3922289A (en) Process and agent for the colorimetric determination of an analyte by means of enzymatic oxidation
ES2108322T3 (es) Procedimiento para la determinacion colorimetrica de un analito mediante (alcohol bencilico)-deshidrogenasa y un indicador de redox cromogeno.
JPS5783287A (en) Elimination of hydrogen peroxide
ATE160590T1 (de) Einstufentest für aspartat-aminotransferase
ATE280241T1 (de) Chemilumineszierende zusammensetzungen und entsprechende verfahren
WO1998023716A3 (en) Enzymatic oxidation process
DK0784674T3 (da) Hidtil ukendt lipolytisk enzym
AU1069483A (en) P-nitrophenol modified substrate and temperature independent buffer
Marklund Mechanisms of the irreversible inactivation of horseradish peroxidase caused by hydroxymethylhydroperoxide
Akio et al. Comparative studies on cholesterol oxidases from different sources
EP0078703A3 (de) Markierte Pyroglu-L-Phe-L-Arg-Derivate, Substrate und Methoden zur Bestimmung von Kallikrein
ATE63334T1 (de) Pyruvatoxidase.
DE69433003D1 (de) Assayverfahren für biologische Komponenten
DE3466708D1 (en) Process for the enzymatic determination of sulphite
Roy et al. An indirect free radical-based assay for the enzyme cellobiose: quinone oxidoreductase
Luzikov et al. Studies on stabilization of the oxidative phosphorylation system. II. Electron transfer-dependent resistance of succinate oxidase and NADH oxidase systems of submitochondrial particles to proteinases and cobra venom phospholipase
CA2251803A1 (en) Assay of peroxidase activity
JPS561896A (en) Determination of hydrogen peroxide and reagent for it
Visser et al. The protein composition of the cytoplasmic membrane of aerobically and anaerobically grown Escherichia coli
JPS57163497A (en) Determination of substrate or enzyme activity
EP0411604A2 (de) Verfahren und Satz zur Bestimmung von Bestandteilen
AU625540B2 (en) High sensitivity detection of peroxidase activity

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee