DE3028569A1 - Biophysikochemische anordnung, verfahren zu ihrer herstellung sowie fuehler mit einer solchen anordnung - Google Patents
Biophysikochemische anordnung, verfahren zu ihrer herstellung sowie fuehler mit einer solchen anordnungInfo
- Publication number
- DE3028569A1 DE3028569A1 DE19803028569 DE3028569A DE3028569A1 DE 3028569 A1 DE3028569 A1 DE 3028569A1 DE 19803028569 DE19803028569 DE 19803028569 DE 3028569 A DE3028569 A DE 3028569A DE 3028569 A1 DE3028569 A1 DE 3028569A1
- Authority
- DE
- Germany
- Prior art keywords
- biophysicochemical
- membrane
- arrangement
- enzyme
- molecules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012528 membrane Substances 0.000 title claims abstract description 59
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 52
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 52
- 230000008859 change Effects 0.000 title claims abstract description 5
- 239000000126 substance Substances 0.000 title description 2
- 239000000758 substrate Substances 0.000 claims abstract description 49
- 230000004044 response Effects 0.000 claims abstract description 3
- 229940088598 enzyme Drugs 0.000 claims description 48
- 239000007788 liquid Substances 0.000 claims description 31
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical group CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 claims description 20
- 229960004373 acetylcholine Drugs 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 102000012440 Acetylcholinesterase Human genes 0.000 claims description 12
- 108010022752 Acetylcholinesterase Proteins 0.000 claims description 12
- 150000002632 lipids Chemical class 0.000 claims description 12
- 238000005192 partition Methods 0.000 claims description 12
- 229940022698 acetylcholinesterase Drugs 0.000 claims description 11
- 238000003860 storage Methods 0.000 claims description 7
- 230000005684 electric field Effects 0.000 claims description 5
- 150000002500 ions Chemical class 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 239000012736 aqueous medium Substances 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 239000007795 chemical reaction product Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 238000001042 affinity chromatography Methods 0.000 claims description 2
- 230000003197 catalytic effect Effects 0.000 claims description 2
- -1 hydrogen ions Chemical class 0.000 claims description 2
- 238000013508 migration Methods 0.000 claims description 2
- 230000005012 migration Effects 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 230000007480 spreading Effects 0.000 claims description 2
- 238000003892 spreading Methods 0.000 claims description 2
- KILNVBDSWZSGLL-KXQOOQHDSA-N 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCC KILNVBDSWZSGLL-KXQOOQHDSA-N 0.000 claims 1
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims 1
- 108091006112 ATPases Proteins 0.000 claims 1
- 102000057290 Adenosine Triphosphatases Human genes 0.000 claims 1
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims 1
- 238000006555 catalytic reaction Methods 0.000 claims 1
- 239000003989 dielectric material Substances 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 210000000056 organ Anatomy 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- 238000009792 diffusion process Methods 0.000 abstract description 8
- 238000006911 enzymatic reaction Methods 0.000 abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 239000010410 layer Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 9
- 239000011888 foil Substances 0.000 description 6
- 238000011161 development Methods 0.000 description 5
- 238000011835 investigation Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 210000005036 nerve Anatomy 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000003421 catalytic decomposition reaction Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 241000252073 Anguilliformes Species 0.000 description 1
- 241000277305 Electrophorus electricus Species 0.000 description 1
- 239000000232 Lipid Bilayer Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 230000003376 axonal effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 125000003473 lipid group Chemical group 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000002831 pharmacologic agent Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000000946 synaptic effect Effects 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Electromagnetism (AREA)
- Cell Biology (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19803028569 DE3028569A1 (de) | 1980-07-28 | 1980-07-28 | Biophysikochemische anordnung, verfahren zu ihrer herstellung sowie fuehler mit einer solchen anordnung |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19803028569 DE3028569A1 (de) | 1980-07-28 | 1980-07-28 | Biophysikochemische anordnung, verfahren zu ihrer herstellung sowie fuehler mit einer solchen anordnung |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3028569A1 true DE3028569A1 (de) | 1982-02-25 |
| DE3028569C2 DE3028569C2 (enrdf_load_stackoverflow) | 1990-03-22 |
Family
ID=6108283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19803028569 Granted DE3028569A1 (de) | 1980-07-28 | 1980-07-28 | Biophysikochemische anordnung, verfahren zu ihrer herstellung sowie fuehler mit einer solchen anordnung |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3028569A1 (enrdf_load_stackoverflow) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994023295A1 (en) * | 1993-04-07 | 1994-10-13 | Ecossensors Limited | Biological species detection method and biosensor therefor |
| EP0815438A4 (en) * | 1995-03-17 | 2001-04-18 | Harvard College | CHARACTERIZATION OF PARTICULAR POLYMER MOLECULES ON THE BASIS OF MONOMER-INTERFACE INTERACTIONS |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6627067B1 (en) | 1999-06-22 | 2003-09-30 | President And Fellows Of Harvard College | Molecular and atomic scale evaluation of biopolymers |
| US7846738B2 (en) | 2003-08-15 | 2010-12-07 | President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US7947454B2 (en) | 2004-03-23 | 2011-05-24 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US8828211B2 (en) | 2010-06-08 | 2014-09-09 | President And Fellows Of Harvard College | Nanopore device with graphene supported artificial lipid membrane |
| US8986528B2 (en) | 1995-03-17 | 2015-03-24 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
-
1980
- 1980-07-28 DE DE19803028569 patent/DE3028569A1/de active Granted
Non-Patent Citations (1)
| Title |
|---|
| Zeitschr. Experientia, Bd. 28, 1972, S. 256, 257 * |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994023295A1 (en) * | 1993-04-07 | 1994-10-13 | Ecossensors Limited | Biological species detection method and biosensor therefor |
| GB2291714A (en) * | 1993-04-07 | 1996-01-31 | Ecossensors Ltd | Biological species detection method and biosensor therefor |
| GB2291714B (en) * | 1993-04-07 | 1997-03-05 | Ecossensors Ltd | Biological species detection method and biosensor therefor |
| EP1956367A1 (en) * | 1995-03-17 | 2008-08-13 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6673615B2 (en) | 1995-03-17 | 2004-01-06 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US7189503B2 (en) | 1995-03-17 | 2007-03-13 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| EP0815438A4 (en) * | 1995-03-17 | 2001-04-18 | Harvard College | CHARACTERIZATION OF PARTICULAR POLYMER MOLECULES ON THE BASIS OF MONOMER-INTERFACE INTERACTIONS |
| US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US9046483B2 (en) | 1995-03-17 | 2015-06-02 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US8986528B2 (en) | 1995-03-17 | 2015-03-24 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
| US6627067B1 (en) | 1999-06-22 | 2003-09-30 | President And Fellows Of Harvard College | Molecular and atomic scale evaluation of biopolymers |
| US8969091B2 (en) | 2003-08-15 | 2015-03-03 | President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US7846738B2 (en) | 2003-08-15 | 2010-12-07 | President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US8394640B2 (en) | 2003-08-15 | 2013-03-12 | President And Fellows Of Harvard College | Study of polymer molecules and conformations with a nanopore |
| US7947454B2 (en) | 2004-03-23 | 2011-05-24 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US8673556B2 (en) | 2004-03-23 | 2014-03-18 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| US8828211B2 (en) | 2010-06-08 | 2014-09-09 | President And Fellows Of Harvard College | Nanopore device with graphene supported artificial lipid membrane |
| US9797863B2 (en) | 2010-06-08 | 2017-10-24 | President And Fellows Of Harvard College | Graphene supported artificial membranes and uses thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3028569C2 (enrdf_load_stackoverflow) | 1990-03-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8125 | Change of the main classification |
Ipc: G01N 27/40 |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |