DE301590C - - Google Patents
Info
- Publication number
- DE301590C DE301590C DENDAT301590D DE301590DA DE301590C DE 301590 C DE301590 C DE 301590C DE NDAT301590 D DENDAT301590 D DE NDAT301590D DE 301590D A DE301590D A DE 301590DA DE 301590 C DE301590 C DE 301590C
- Authority
- DE
- Germany
- Prior art keywords
- acid
- phosphoric acid
- fermentation
- parts
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 239000001263 FEMA 3042 Substances 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 229940033123 Tannic Acid Drugs 0.000 claims description 3
- LRBQNJMCXXYXIU-NRMVVENXSA-N Tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 claims description 3
- -1 carbohydrate phosphoric acid esters Chemical class 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 229910052816 inorganic phosphate Inorganic materials 0.000 claims description 3
- 235000015523 tannic acid Nutrition 0.000 claims description 3
- 229920002258 tannic acid Polymers 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K [O-]P([O-])([O-])=O Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000011707 mineral Substances 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 235000021317 phosphate Nutrition 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 150000007513 acids Chemical class 0.000 claims 1
- 239000000243 solution Substances 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N HCl Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- YCPXWRQRBFJBPZ-UHFFFAOYSA-N 5-Sulfosalicylic acid Chemical compound OC(=O)C1=CC(S(O)(=O)=O)=CC=C1O YCPXWRQRBFJBPZ-UHFFFAOYSA-N 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 159000000007 calcium salts Chemical class 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N D-sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229960004793 Sucrose Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L cacl2 Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- SLAMLWHELXOEJZ-UHFFFAOYSA-N o-Nitrobenzoic acid Chemical compound OC(=O)C1=CC=CC=C1[N+]([O-])=O SLAMLWHELXOEJZ-UHFFFAOYSA-N 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H11/00—Compounds containing saccharide radicals esterified by inorganic acids; Metal salts thereof
- C07H11/04—Phosphates; Phosphites; Polyphosphates
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
KAISERLICHESIMPERIAL
PATENTAMT.PATENT OFFICE.
Durch das Hauptpatent ist ein Verfahren zur Darstellung von Kohlehydratphosphorsäureestern aus gärungsfähigem Material und anorganischen Phosphaten unter dem Einfluß der Hefefermente geschützt, das dadurch ge-• kennzeichnet ist, daß man nach vollendeter Phosphatbindung die Unterbrechung der Fermenttätigkeit gleichzeitig mit der Fällung der in Lösung befindlichen Eiweißkörper statt durch" Erwärmen, wie man dies früher bereits getan hatte, durch Zusatz von Gerbsäure bewirkt. The main patent is a process for the preparation of carbohydrate phosphoric acid esters of fermentable material and inorganic phosphates under the influence of the yeast fermentation, which is characterized by the fact that one after finished Phosphate binding the interruption of fermentation activity simultaneously with the precipitation of the protein bodies in solution instead of "heating", as was done earlier done by adding tannic acid.
Es wurde nun weiter gefunden, daß man an Stelle von Gerbsäure auch, die anderen übliehen chemischen Eiweißfällungsmittel zur Unterbrechung- der Fermenttätigkeit verwenden kann, was mit Rücksicht auf die in dem Hauptpatent angeführte Literatur nicht vorausgesehen werden konnte. ·It has now been found that, instead of tannic acid, one also disapproves of the others Use chemical protein precipitants to interrupt the fermentation activity can, which was not foreseen with regard to the literature cited in the main patent could be. ·
Ein besonderer Vorteil ergibt sich aus der Benutzung der anorganischen Säuren, deren Verwendbarkeit im vorliegenden Fall umso überraschender ist, als man in mineralsaurer Lösung eine teilweise Hydrolyse der Kohlehydratphosphorsäureester und damit eine starke Verunreinigung der zu isolierenden Salze mit anorganischen Phosphaten befürchten mußte. A particular advantage results from the use of the inorganic acids, their Usability in the present case is all the more surprising as one in mineral acid Solution a partial hydrolysis of the carbohydrate phosphoric acid ester and thus a had to fear strong contamination of the salts to be isolated with inorganic phosphates.
Beispiel ι.Example ι.
Zu einer gemäß dem Beispiel des Hauptpatents gewonnenen Reaktionslösung aus insgesamt 125 Teilen Rohrzucker, 50 Teilen Trockenhefe, 100 Teilen Dinatriumphosphat und 750 Teilen Wasser fügt man unter Umrühren etwa 50 Teile einer ungefähr 3oprozentigen wässerigen Salzsäure. Nach einigem Stehen wird die geklärte Lösung abgenutscht, das Filtrat neutralisiert und mit Chlorcalciumlösung versetzt. Das Calciumsalz der Esterphosphorsäure fällt teilweise unmittelbar aus. Diese Fällung kann durch Ausrühren der Lösung bei mäßig erhöhter Temperatur zu einer fast quantitativen gestaltet werden.To a reaction solution obtained according to the example of the main patent from a total of 125 parts cane sugar, 50 parts dry yeast, 100 parts disodium phosphate and 750 parts of water are added with stirring about 50 parts of an approximately 3% strength aqueous hydrochloric acid. After standing for a while, the clarified solution is filtered off with suction, the filtrate is neutralized and treated with calcium chloride solution offset. The calcium salt of the ester phosphoric acid partially precipitates directly. This precipitation can be increased by stirring the solution at a moderately elevated temperature an almost quantitative one.
■ο ·■ · 1■ ο · ■ · 1
Ersetzt man im Beispiel 1 die wässerige Salzsäure durch 80 Teile Sulfosalicylsäure in 5prozentiger wässeriger Lösung, und arbeitet man im übrigen wie dort angegeben, so erhält man gleichfalls das Calciumsalz der ■Esterphosphorsäure in guter Ausbeute und reiner Form.In Example 1, if the aqueous hydrochloric acid is replaced by 80 parts of sulfosalicylic acid in 5 percent aqueous solution, and if you work otherwise as indicated there, then you get the calcium salt of the ester phosphoric acid is also obtained in good yield and purer Shape.
Statt der Sulfosalicylsäure können andere Eiweißfällungsmittel, wie die 2-Nitrobenzoesäure, benutzt werden.Instead of sulfosalicylic acid, other protein precipitating agents such as 2-nitrobenzoic acid, to be used.
Claims (1)
292817 geschützten Verfahrens zur Darstellung .·; von Kohlehydratphosphorsäureestern .-aus gärungsfähigem Material und
anorganischen Phosphaten unter dem EinEmbodiment of the patent
292817 protected method for representation. ·; of carbohydrate phosphoric acid esters.-from fermentable material and
inorganic phosphates under the one
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE301590C true DE301590C (en) |
Family
ID=555455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DENDAT301590D Active DE301590C (en) |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE301590C (en) |
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0
- DE DENDAT301590D patent/DE301590C/de active Active
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